[Objective] The aim was to separate chromium-resistant microorganism from soil contaminated by chromium.[Method] Separation and purification technique was used as follows：different concentrations of Cr^6＋ were added...[Objective] The aim was to separate chromium-resistant microorganism from soil contaminated by chromium.[Method] Separation and purification technique was used as follows：different concentrations of Cr^6＋ were added into medium,and chromium-resistant fungi were screened after separations and domestications.The selected fungi were under preliminary identification according to its morphological and colony characteristics.Then,related biological characteristics were studied,including measurement of growth curve,growing effects by temperature,pH value and osmotic pressure.[Result] The Cr（VI） with concentration of 1 000 mg/L was separated and selected from soils in ten different places contaminated seriously by heavy metal in adjacent region of Yulin City.Considering its morphological and colony characteristics,it was preliminarily identified as saccharomycetes,which can well grow within 15-37 ℃,and whose most suitable temperature was 28℃.Bacterial strain can grow well with pH of 4-10,and the optimum pH was 7.2;besides,it can grow well with NaCl concentration of 0.5%-5.0%.Through the experiment,the bacteria was found with resistance not only to chromium,but also to heavy metals such as Pb＋Cu,Cu＋Fe,Pb＋Fe,and Pb＋Cu＋Fe.[Conclusion] The fungi selected from the experiment were of good adaptability to natural environment,and it also had resistance to other heavy metals.展开更多
基金Supported by Chinese National Natural Science Foundation(30960008 )Educational Commission of Guangxi Province(200810LX393)+2 种基金Starting Project of Yulin Normal College,Guangxi ProvinceSpecialized Research Project of Yulin Normal College,Guangxi Province (2011YJZX01)Project Supported by the Science Foundation for Young Scientists of Guangxi Yulin Normal College (2010YJQN24)~~
文摘[Objective] The aim was to separate chromium-resistant microorganism from soil contaminated by chromium.[Method] Separation and purification technique was used as follows：different concentrations of Cr^6＋ were added into medium,and chromium-resistant fungi were screened after separations and domestications.The selected fungi were under preliminary identification according to its morphological and colony characteristics.Then,related biological characteristics were studied,including measurement of growth curve,growing effects by temperature,pH value and osmotic pressure.[Result] The Cr（VI） with concentration of 1 000 mg/L was separated and selected from soils in ten different places contaminated seriously by heavy metal in adjacent region of Yulin City.Considering its morphological and colony characteristics,it was preliminarily identified as saccharomycetes,which can well grow within 15-37 ℃,and whose most suitable temperature was 28℃.Bacterial strain can grow well with pH of 4-10,and the optimum pH was 7.2;besides,it can grow well with NaCl concentration of 0.5%-5.0%.Through the experiment,the bacteria was found with resistance not only to chromium,but also to heavy metals such as Pb＋Cu,Cu＋Fe,Pb＋Fe,and Pb＋Cu＋Fe.[Conclusion] The fungi selected from the experiment were of good adaptability to natural environment,and it also had resistance to other heavy metals.
