Objective To study the effects of emodin on proliferation and differentiation of 3T3 L1 preadipocyte and the possible mechanism Methods Cell proliferation was determined by MTT spectrophotometry, cell differentiat...Objective To study the effects of emodin on proliferation and differentiation of 3T3 L1 preadipocyte and the possible mechanism Methods Cell proliferation was determined by MTT spectrophotometry, cell differentiation was determined by Oil Red O staining, and fatty acid synthase (FAS) activity was determined by spectrophotometry Results Emodin promoted proliferation of 3T3 L1 preadipocyte at low concentration and inhibited the proliferation at high concentration in a dose related manner In contrast, it inhibited cell differentiation into adipocyte at low concentration in a dose related manner In vitro emodin inhibited the activity of FAS in a dose related manner Conclusions The effects of emodin on 3T3 L1 cell's proliferation and differentiation are dose dependent Emodin inhibits the activity of FAS Our results suggest that emodin should have a potential to serve as a fat reducing drug展开更多
文摘Objective To study the effects of emodin on proliferation and differentiation of 3T3 L1 preadipocyte and the possible mechanism Methods Cell proliferation was determined by MTT spectrophotometry, cell differentiation was determined by Oil Red O staining, and fatty acid synthase (FAS) activity was determined by spectrophotometry Results Emodin promoted proliferation of 3T3 L1 preadipocyte at low concentration and inhibited the proliferation at high concentration in a dose related manner In contrast, it inhibited cell differentiation into adipocyte at low concentration in a dose related manner In vitro emodin inhibited the activity of FAS in a dose related manner Conclusions The effects of emodin on 3T3 L1 cell's proliferation and differentiation are dose dependent Emodin inhibits the activity of FAS Our results suggest that emodin should have a potential to serve as a fat reducing drug
