目的研究洋葱总黄酮对糖尿病大鼠血清及视网膜的抗氧化损伤作用。方法用乙醇回流法提取洋葱总黄酮。建立链脲佐菌素诱导的糖尿病大鼠模型。50只雄性SPF级大鼠按随机数字表法分为正常组、模型组和3个剂量实验组,每组10只。实验组,每日1...目的研究洋葱总黄酮对糖尿病大鼠血清及视网膜的抗氧化损伤作用。方法用乙醇回流法提取洋葱总黄酮。建立链脲佐菌素诱导的糖尿病大鼠模型。50只雄性SPF级大鼠按随机数字表法分为正常组、模型组和3个剂量实验组,每组10只。实验组,每日1次灌胃3个剂量(200,100,50mg·kg^(-1))洋葱总黄酮;模型组和正常组,每日给予等体积的生理盐水。测定大鼠的血糖和体重。用硫代巴比妥酸法和黄瞟呤氧化酶法检测各组大鼠血清及视网膜组织的丙二醛(MDA)含量和超氧化物歧化酶(SOD)活性。结果与模型组比较,3个剂量实验组的血糖均明显降低、体重均明显增加(均P<0.05)。血清:与模型组相比,3个剂量实验组MDA含量(nmol·mL^(-1);18.75±2.02 vs 15.36±1.56,12.75±1.78,9.45±2.23)明显降低,而SOD活力(U·mL^(-1);223.78±25.69 vs298.43±19.72,348.16±23.74,382.43±18.46)明显增加(均P<0.01)。视网膜:与模型组相比,3个剂量实验组MDA明显降低,而SOD活力明显增加(均P<0.01)。结论洋葱总黄酮可显著降低糖尿病大鼠的氧化应激水平,增强糖尿病大鼠血清及视网膜的抗氧化能力。展开更多
To explore the biological effects of light on trabecular cells, cultured bovine trabecular cells were exposed to visible light of different wavelength with different energy. Cellular morphology, structure, proliferat...To explore the biological effects of light on trabecular cells, cultured bovine trabecular cells were exposed to visible light of different wavelength with different energy. Cellular morphology, structure, proliferation, and phagocytosis were observed. The cells showed no remarkable changes when the energy was low. When the exposure energy reached 1.12 mW/cm 2, the cytoplasm showed a rough appearance, and cell proliferation and phagocytosis decreased. This phototoxicity was strong with white light (compound chromatic light), moderate with violet light or yellow light, and mild with red light.展开更多
文摘目的研究洋葱总黄酮对糖尿病大鼠血清及视网膜的抗氧化损伤作用。方法用乙醇回流法提取洋葱总黄酮。建立链脲佐菌素诱导的糖尿病大鼠模型。50只雄性SPF级大鼠按随机数字表法分为正常组、模型组和3个剂量实验组,每组10只。实验组,每日1次灌胃3个剂量(200,100,50mg·kg^(-1))洋葱总黄酮;模型组和正常组,每日给予等体积的生理盐水。测定大鼠的血糖和体重。用硫代巴比妥酸法和黄瞟呤氧化酶法检测各组大鼠血清及视网膜组织的丙二醛(MDA)含量和超氧化物歧化酶(SOD)活性。结果与模型组比较,3个剂量实验组的血糖均明显降低、体重均明显增加(均P<0.05)。血清:与模型组相比,3个剂量实验组MDA含量(nmol·mL^(-1);18.75±2.02 vs 15.36±1.56,12.75±1.78,9.45±2.23)明显降低,而SOD活力(U·mL^(-1);223.78±25.69 vs298.43±19.72,348.16±23.74,382.43±18.46)明显增加(均P<0.01)。视网膜:与模型组相比,3个剂量实验组MDA明显降低,而SOD活力明显增加(均P<0.01)。结论洋葱总黄酮可显著降低糖尿病大鼠的氧化应激水平,增强糖尿病大鼠血清及视网膜的抗氧化能力。
文摘To explore the biological effects of light on trabecular cells, cultured bovine trabecular cells were exposed to visible light of different wavelength with different energy. Cellular morphology, structure, proliferation, and phagocytosis were observed. The cells showed no remarkable changes when the energy was low. When the exposure energy reached 1.12 mW/cm 2, the cytoplasm showed a rough appearance, and cell proliferation and phagocytosis decreased. This phototoxicity was strong with white light (compound chromatic light), moderate with violet light or yellow light, and mild with red light.
