目的:比较4种不同的粘接系统对正常牙本质和龋损影响牙本质的粘接强度。方法:24颗近中面中龋的人离体磨牙,在龋损检知液监控下用慢速球钻去龋,在远中面健康牙本质上制备相同深度的窝洞。随机分为4组,分别用4种粘接系统(Prime&Bo...目的:比较4种不同的粘接系统对正常牙本质和龋损影响牙本质的粘接强度。方法:24颗近中面中龋的人离体磨牙,在龋损检知液监控下用慢速球钻去龋,在远中面健康牙本质上制备相同深度的窝洞。随机分为4组,分别用4种粘接系统(Prime&Bond NT,Adper Single Bond 2,Adper Prompt和XenoⅢ)按照使用说明进行粘接,用树脂(FiltekP60)修复后保存于37℃的水中。沿牙长轴方向将树脂和牙齿从颊舌方向片切,厚度约为1.0mm,并修整成粘接面(正常牙本质或龋损影响牙本质)面积约1mm2的试件,进行微拉伸实验,并用扫描电镜观察粘接面形态。结果:4种粘接系统在正常牙本质上获得的粘接强度均强于在龋损影响牙本质上获得的强度(P<0.05);2种全酸蚀粘接系统在2种牙本质上获得的粘接强度均强于2种自酸蚀粘接系统获得的强度(P<0.05)。扫描电镜观察,混合层在龋损影响牙本质上表现得厚但更疏松;与正常牙本质相比,龋损影响牙本质上形成的树脂突少。结论:2种全酸蚀粘接系统无论在正常牙本质还是龋损影响牙本质上均能获得比2种自酸蚀粘接系统更强的粘接力。展开更多
Eighteen gametophytes includingL. japonica, L. ochotensisandL. longissima, were verified with random amplified polymorphic DNA (RAPD) technique. Eighteen ten-base primers were chosen from 100 primers selected for fina...Eighteen gametophytes includingL. japonica, L. ochotensisandL. longissima, were verified with random amplified polymorphic DNA (RAPD) technique. Eighteen ten-base primers were chosen from 100 primers selected for final amplification test. Among the total of 205 bands amplified, 181 (88.3%) were polymorphic. The genetic distance among different strains ranged from 0.072 to 0.391. The dendrogram constructed by unweighted pair-group method with arithmetic (UPGMA) method showed that the female and male gametophytes of the same cell lines could be grouped in pairs respectively. It indicated that RAPD analysis could be used not only to distinguish different strains ofLaminaria, but also to distinguish male and female gametophyte within the same cell lines. There is ambiguous systematic relationship if judged merely by the present data. It seems that the use of RAPD marker is limited to elucidation of the phylogenetic relationship among the species ofLaminaria.展开更多
文摘目的:比较4种不同的粘接系统对正常牙本质和龋损影响牙本质的粘接强度。方法:24颗近中面中龋的人离体磨牙,在龋损检知液监控下用慢速球钻去龋,在远中面健康牙本质上制备相同深度的窝洞。随机分为4组,分别用4种粘接系统(Prime&Bond NT,Adper Single Bond 2,Adper Prompt和XenoⅢ)按照使用说明进行粘接,用树脂(FiltekP60)修复后保存于37℃的水中。沿牙长轴方向将树脂和牙齿从颊舌方向片切,厚度约为1.0mm,并修整成粘接面(正常牙本质或龋损影响牙本质)面积约1mm2的试件,进行微拉伸实验,并用扫描电镜观察粘接面形态。结果:4种粘接系统在正常牙本质上获得的粘接强度均强于在龋损影响牙本质上获得的强度(P<0.05);2种全酸蚀粘接系统在2种牙本质上获得的粘接强度均强于2种自酸蚀粘接系统获得的强度(P<0.05)。扫描电镜观察,混合层在龋损影响牙本质上表现得厚但更疏松;与正常牙本质相比,龋损影响牙本质上形成的树脂突少。结论:2种全酸蚀粘接系统无论在正常牙本质还是龋损影响牙本质上均能获得比2种自酸蚀粘接系统更强的粘接力。
文摘Eighteen gametophytes includingL. japonica, L. ochotensisandL. longissima, were verified with random amplified polymorphic DNA (RAPD) technique. Eighteen ten-base primers were chosen from 100 primers selected for final amplification test. Among the total of 205 bands amplified, 181 (88.3%) were polymorphic. The genetic distance among different strains ranged from 0.072 to 0.391. The dendrogram constructed by unweighted pair-group method with arithmetic (UPGMA) method showed that the female and male gametophytes of the same cell lines could be grouped in pairs respectively. It indicated that RAPD analysis could be used not only to distinguish different strains ofLaminaria, but also to distinguish male and female gametophyte within the same cell lines. There is ambiguous systematic relationship if judged merely by the present data. It seems that the use of RAPD marker is limited to elucidation of the phylogenetic relationship among the species ofLaminaria.
