目的:观察姜黄素对坐骨神经慢性压迫性损伤(CCI)大鼠痛行为及脊髓背角和背根节趋化因子受体CX3CR1表达的影响。方法:雄性SD大鼠72只,随机分为假手术组(Sham组)、CCI组、姜黄素组(Cur组)、溶剂对照组(SC组)。各组分别于术前2 d和术后1,3,...目的:观察姜黄素对坐骨神经慢性压迫性损伤(CCI)大鼠痛行为及脊髓背角和背根节趋化因子受体CX3CR1表达的影响。方法:雄性SD大鼠72只,随机分为假手术组(Sham组)、CCI组、姜黄素组(Cur组)、溶剂对照组(SC组)。各组分别于术前2 d和术后1,3,5,7,10,14 d测定大鼠后足热缩足反射潜伏期(TWL)和机械缩足反射阈值(MWT);在术后3,7,14 d处死大鼠(n=6),分别制备大鼠L4-5节段脊髓和背根节的石蜡标本,用免疫组织化学法测定CX3CR1在脊髓背角和背根节的动态变化。结果:与Sham组比较,CCI组术后各时间点TWL,MWT明显降低(P<0.01),且在术后3 d都降至最低;与CCI组比较,Cur组术后7,10,14 d TWL明显增高(P<0.05),术后10,14 d MWT明显增高(P<0.05)。同时,CCI大鼠术侧脊髓背角浅层和背根节的CX3CR1阳性细胞数明显增多,而姜黄素能够明显减少CX3CR1阳性细胞的表达。结论:姜黄素能减轻神经病理性疼痛,其机制可能与减少脊髓背角和背根节CX3CR1的表达有关。展开更多
Objective：To investigate the effects of curcumin on pain threshold and the expressions of nuclear factorκB（NF-κB）and CX3C chemokine receptor 1（CX3CR1）in spinal cord and dorsal root ganglion（DRG）of the rats wi...Objective：To investigate the effects of curcumin on pain threshold and the expressions of nuclear factorκB（NF-κB）and CX3C chemokine receptor 1（CX3CR1）in spinal cord and dorsal root ganglion（DRG）of the rats with sciatic nerve chronic constrictive injury.Methods：One hundred and twenty male Sprague Dawley rats,weighing 220-250 g,were randomly divided into 4 groups.Sham surgery（sham）group：the sciatic nerves of rats were only made apart but not ligated;chronic constrictive injury（CCI）group：the sciatic nerves of rats were only ligated without any drug treatment;curcumin treated injury（Cur）model group：the rats were administrated with curcumin 100 mg/（kg·d）by intraperitoneal injection for 14 days after CCI;solvent control（SC）group：the rats were administrated with the solvent at the same dose for 14 days after CCI.Thermal withdrawal latency（TWL）and mechanical withdrawal threshold（MWT）of rats were respectively measured on pre-operative day 2 and postoperative day 1,3,5,7,10 and 14.The lumbar segment L4-5 of the spinal cord and the L4,L5 DRG was removed at post-operative day 3,7 and 14.The change of nuclear factorκB（NF-κB）p65 expression was detected by Western blotting while the expression of CX3CR1 was determined by immunohistochemical staining.Results：Compared with the sham group,the TWL and MWT of rats in the CCI group were significantly decreased on each post-operative day（P〈0.01）,which reached a nadir on the 3rd day after CCI,and the expressions of NF-κB p65and CX3CR1 were markedly increased in spinal cord dorsal horn and DRG.In the Cur group,the TWL of rats were significantly increased than those in the CCI group on post-operative day 7,10 and 14（P〈0.05）and MWT increased than those in the CCI group on post-operative day 10 and 14（P〈0.05）.In addition,the administration of curcumin significantly decreased the positive expressions of NF-κB p65 and CX3CR1 in spinal cord and DRG（P〈0.05）.Conclusion：Our study suggests that curcumin cou展开更多
文摘目的:观察姜黄素对坐骨神经慢性压迫性损伤(CCI)大鼠痛行为及脊髓背角和背根节趋化因子受体CX3CR1表达的影响。方法:雄性SD大鼠72只,随机分为假手术组(Sham组)、CCI组、姜黄素组(Cur组)、溶剂对照组(SC组)。各组分别于术前2 d和术后1,3,5,7,10,14 d测定大鼠后足热缩足反射潜伏期(TWL)和机械缩足反射阈值(MWT);在术后3,7,14 d处死大鼠(n=6),分别制备大鼠L4-5节段脊髓和背根节的石蜡标本,用免疫组织化学法测定CX3CR1在脊髓背角和背根节的动态变化。结果:与Sham组比较,CCI组术后各时间点TWL,MWT明显降低(P<0.01),且在术后3 d都降至最低;与CCI组比较,Cur组术后7,10,14 d TWL明显增高(P<0.05),术后10,14 d MWT明显增高(P<0.05)。同时,CCI大鼠术侧脊髓背角浅层和背根节的CX3CR1阳性细胞数明显增多,而姜黄素能够明显减少CX3CR1阳性细胞的表达。结论:姜黄素能减轻神经病理性疼痛,其机制可能与减少脊髓背角和背根节CX3CR1的表达有关。
基金Supported by National Natural Science Foundation of China(No.81073125)Natural Science Foundation of Zhejiang Province(No.Y2090252)International Cooperation Project of Wenzhou Science and Technology Bureau(No.H20070035)
文摘Objective：To investigate the effects of curcumin on pain threshold and the expressions of nuclear factorκB（NF-κB）and CX3C chemokine receptor 1（CX3CR1）in spinal cord and dorsal root ganglion（DRG）of the rats with sciatic nerve chronic constrictive injury.Methods：One hundred and twenty male Sprague Dawley rats,weighing 220-250 g,were randomly divided into 4 groups.Sham surgery（sham）group：the sciatic nerves of rats were only made apart but not ligated;chronic constrictive injury（CCI）group：the sciatic nerves of rats were only ligated without any drug treatment;curcumin treated injury（Cur）model group：the rats were administrated with curcumin 100 mg/（kg·d）by intraperitoneal injection for 14 days after CCI;solvent control（SC）group：the rats were administrated with the solvent at the same dose for 14 days after CCI.Thermal withdrawal latency（TWL）and mechanical withdrawal threshold（MWT）of rats were respectively measured on pre-operative day 2 and postoperative day 1,3,5,7,10 and 14.The lumbar segment L4-5 of the spinal cord and the L4,L5 DRG was removed at post-operative day 3,7 and 14.The change of nuclear factorκB（NF-κB）p65 expression was detected by Western blotting while the expression of CX3CR1 was determined by immunohistochemical staining.Results：Compared with the sham group,the TWL and MWT of rats in the CCI group were significantly decreased on each post-operative day（P〈0.01）,which reached a nadir on the 3rd day after CCI,and the expressions of NF-κB p65and CX3CR1 were markedly increased in spinal cord dorsal horn and DRG.In the Cur group,the TWL of rats were significantly increased than those in the CCI group on post-operative day 7,10 and 14（P〈0.05）and MWT increased than those in the CCI group on post-operative day 10 and 14（P〈0.05）.In addition,the administration of curcumin significantly decreased the positive expressions of NF-κB p65 and CX3CR1 in spinal cord and DRG（P〈0.05）.Conclusion：Our study suggests that curcumin cou
