To determine the biochemical changes of affected hip in Perthes disease and control cartilage as a first step in investigating the role of cartilage alterations in the pathogenesis of the disease, pathological cartila...To determine the biochemical changes of affected hip in Perthes disease and control cartilage as a first step in investigating the role of cartilage alterations in the pathogenesis of the disease, pathological cartilage was collected from 20 patients suffered from Perthes disease with stagesⅡ ~ Ⅲ (classified according to Caterall). Using identical techniques, appereutly healthy cartilage ofthe same site was obtained from 10 cadavers whose age matched with the former groups. The threecomponents of cartilage proteoglycans (PG): hexosamine, hexuronic acid, sulfate radical, level ofhyaluronic acid (HA) in synovial fluid and serum were detected by radioimmunoassay. The correlation among HA level in synovial fluid, serum and PG content in cartilage were analyzed. The presentstudies showed that ①Compared with the normal autopsy specimens, Perthes disease specimens had asignificantly decreased PG content (P < 0. 01). ②Children sufrered from Perthes' disease had amuch higher level of HA in serum than the controls (P < 0. 01). HA level in serum lead a inversecorrelation with that in synovial fluid (r =- 0. 663,P< 0. 05).③There was a positive correlationbetween HA level in synovial rluid and PG toment (r = 0. 682,P < 0. 05). PG content was negativelycorrelated with HA level in serum of perthes disease (r=- 0. 632,P< 0. 05). 'The results suggestthat ① Children arfected with Perthes disase lead a markedly dysfunctional hip joint. ② To someextent serum level of HA reflect biocbemical changes in cartilage in Perthes disease. ③ Disturbanceof arftcted joint function accelerate the progress or ANFH, which might in turn cause further damage. Hence a cycle of deterioration would be perpetuated.展开更多
文摘To determine the biochemical changes of affected hip in Perthes disease and control cartilage as a first step in investigating the role of cartilage alterations in the pathogenesis of the disease, pathological cartilage was collected from 20 patients suffered from Perthes disease with stagesⅡ ~ Ⅲ (classified according to Caterall). Using identical techniques, appereutly healthy cartilage ofthe same site was obtained from 10 cadavers whose age matched with the former groups. The threecomponents of cartilage proteoglycans (PG): hexosamine, hexuronic acid, sulfate radical, level ofhyaluronic acid (HA) in synovial fluid and serum were detected by radioimmunoassay. The correlation among HA level in synovial fluid, serum and PG content in cartilage were analyzed. The presentstudies showed that ①Compared with the normal autopsy specimens, Perthes disease specimens had asignificantly decreased PG content (P < 0. 01). ②Children sufrered from Perthes' disease had amuch higher level of HA in serum than the controls (P < 0. 01). HA level in serum lead a inversecorrelation with that in synovial fluid (r =- 0. 663,P< 0. 05).③There was a positive correlationbetween HA level in synovial rluid and PG toment (r = 0. 682,P < 0. 05). PG content was negativelycorrelated with HA level in serum of perthes disease (r=- 0. 632,P< 0. 05). 'The results suggestthat ① Children arfected with Perthes disase lead a markedly dysfunctional hip joint. ② To someextent serum level of HA reflect biocbemical changes in cartilage in Perthes disease. ③ Disturbanceof arftcted joint function accelerate the progress or ANFH, which might in turn cause further damage. Hence a cycle of deterioration would be perpetuated.
