Molecularly imprinted polymers(MIPs),as important mimics of antibodies,are chemically synthesized by polymerization in the presence of a target compound.MIPs have found wide applications in important fileds.However,th...Molecularly imprinted polymers(MIPs),as important mimics of antibodies,are chemically synthesized by polymerization in the presence of a target compound.MIPs have found wide applications in important fileds.However,the current molecular imprinting technology suffers from a dilemma;there is often a compromise between the best affinity and the best specificity for MIPs prepared under optimized condi-tions.Herein,we proposed a new strategy called molecular imprinting and cladding(MIC)to solve this issue.The principle is straightforward;after molecular imprinting,a chemically inert cladding thinlayer is generated to precisely cover non-imprinted area.We further proposed a special MIC approach for con-trollably engineering protein binders.The prepared cladded MIPs(cMIPs)exhibited significantly improved affinity and specificity.The general applicability of the proposed strategy and method was ver-ified by engineering of cMIPs for the recognition of a variety of different proteins.The feasibility of cMIPs for real applications was demonstrated by fluorescence imaging of cancer cells against normal cells and immunoassay of C-peptide in human urine.This study opened up a new avenue for controllably engi-neering protein-specific antibody mimics with excellent recognition properties,holding great prospective in important applications such as disease diagnosis and nanomedicine.展开更多
基金the Key Program of the National Natural Science Foundation of China(21834003)the National Science Fund for Distinguished Young Scholars(21425520)the Excellent Research Program(ZYJH004)from Nanjing University to ZL。
文摘Molecularly imprinted polymers(MIPs),as important mimics of antibodies,are chemically synthesized by polymerization in the presence of a target compound.MIPs have found wide applications in important fileds.However,the current molecular imprinting technology suffers from a dilemma;there is often a compromise between the best affinity and the best specificity for MIPs prepared under optimized condi-tions.Herein,we proposed a new strategy called molecular imprinting and cladding(MIC)to solve this issue.The principle is straightforward;after molecular imprinting,a chemically inert cladding thinlayer is generated to precisely cover non-imprinted area.We further proposed a special MIC approach for con-trollably engineering protein binders.The prepared cladded MIPs(cMIPs)exhibited significantly improved affinity and specificity.The general applicability of the proposed strategy and method was ver-ified by engineering of cMIPs for the recognition of a variety of different proteins.The feasibility of cMIPs for real applications was demonstrated by fluorescence imaging of cancer cells against normal cells and immunoassay of C-peptide in human urine.This study opened up a new avenue for controllably engi-neering protein-specific antibody mimics with excellent recognition properties,holding great prospective in important applications such as disease diagnosis and nanomedicine.
基金supported by the Biological Resources Programme,Chinese Academy of Sciences (KFJ-BRP-008-005)Shanghai Science and Technology Development Foundation (21S21901900)+2 种基金the Lingang Laboratory Grant (LG-QS-202206-01)Ministry of Science and Technology of China (2021YFE0111300)National Natural Science Foundation of China (81973513,81573646).
