目的:探讨FoxO4是否通过调控自噬影响小鼠神经干细胞(NSCs)的分化能力,寻找调控NSCs分化潜能的有效途径.方法:利用新生BALB/c小鼠提取海马齿状回(DG)NSCs,通过real time RT-PCR和Western Blot实验检测NSCs在分化前后自噬相关基因以及转...目的:探讨FoxO4是否通过调控自噬影响小鼠神经干细胞(NSCs)的分化能力,寻找调控NSCs分化潜能的有效途径.方法:利用新生BALB/c小鼠提取海马齿状回(DG)NSCs,通过real time RT-PCR和Western Blot实验检测NSCs在分化前后自噬相关基因以及转录因子Forkhead Box O家族(FoxO3、FoxO4和FoxO6)的表达水平;运用基因沉默技术,将电转染后的NSCs分为NC-siRNA组和FoxO4-siRNA组,检测两组NSCs FoxO4 mRNA及蛋白的表达水平,从而验证已转入siRNA序列的目的基因沉默效率;通过real time RT-PCR和Western Blot实验检测NC-siRNA组和FoxO4-siRNA组NSCs自噬相关基因的表达;进一步将两组NSCs分化培养后通过real time RT-PCR实验以及免疫荧光染色检测Tuj1的表达.结果:体外分离培养P0小鼠海马DG区NSCs分化后自噬相关基因ATG3、ATG5、ATG7和Beclin-1 mRNA以及LC3-Ⅱ/Ⅰ蛋白表达水平被观察到均升高(P<0.01),同时还观察到NSCs分化后FoxO3、FoxO4、FoxO6 mRNA的表达水平均增高(P<0.01),其中转录因子FoxO4的mRNA和蛋白表达增高最为显著(P<0.01).通过运用靶向FoxO4的siRNA转染NSCs后,发现NSCs自噬相关基因ATG3、ATG5、ATG7和Beclin-1 mRNA以及LC3-Ⅱ/Ⅰ蛋白表达水平均降低(P<0.05),且NSCs中Tuj1表达受到显著抑制(P<0.05).结论:FoxO4基因沉默降低了小鼠海马NSCs中自噬水平,并抑制其向神经元分化.展开更多
We study the evolution of spectral intensity and degree of coherence of a new class of partially coherent beams,Hermite non-uniformly correlated array beams,in free space and in turbulence,based on the extended Huygen...We study the evolution of spectral intensity and degree of coherence of a new class of partially coherent beams,Hermite non-uniformly correlated array beams,in free space and in turbulence,based on the extended Huygens–Fresnel integral.Such beams possess controllable rectangular grid distributions due to multi-self-focusing propagation property.Furthermore,it is demonstrated that adjusting the initial beam parameters,mode order,shift parameters,array parameters and correlation width plays a role in resisting intensity and degree of coherence degradation effects of the turbulence.展开更多
基金the National Key Research and Development Program of China(Grant No.2019YFA0705000)the National Natural Science Foundation of China(Grant Nos.91750201,11525418,11947240,11974218,12004218,and 11904087)+1 种基金the Local Science and Technology Development Project of the Central Government(Grant No.YDZX20203700001766)Innovation Group of Jinan(Grant No.2018GXRC010)。
文摘We study the evolution of spectral intensity and degree of coherence of a new class of partially coherent beams,Hermite non-uniformly correlated array beams,in free space and in turbulence,based on the extended Huygens–Fresnel integral.Such beams possess controllable rectangular grid distributions due to multi-self-focusing propagation property.Furthermore,it is demonstrated that adjusting the initial beam parameters,mode order,shift parameters,array parameters and correlation width plays a role in resisting intensity and degree of coherence degradation effects of the turbulence.
