Objective： To investigate the effects of electroacupuncture （EA） at Neiguan （PC 6） and Baihui （GV 20） by observing the changes of CCAAT/enhancer-binding protein（C/EBP） homologous protein （CHOP） and caspase-...Objective： To investigate the effects of electroacupuncture （EA） at Neiguan （PC 6） and Baihui （GV 20） by observing the changes of CCAAT/enhancer-binding protein（C/EBP） homologous protein （CHOP） and caspase-12 gene expressions in rats after cerebral ischemia-reperfusion injury （IRI）, and explore whether the apoptosis pathway of endoplasmic reticulum stress （ERS） is involved in the protective mechanisms of EA. Methods： Sixty rats were randomly assigned to five groups （12 in each group）： a normal control group （group A）, a sham-operation group （group B）, an operation group （group C）, an Edaravone group （group D） and an EA group （group E）. The cerebral IRI rat model was induced by middle cerebral artery occlusion （MCAO） using intraluminal monofilament. 2,3,5-triphenyl tetrazolium chloride （TTC） staining was adopted in the measurement of cerebral infarction volume. Real-time polymerase chain reaction （RT-PCR） was used to determine the mRNA expressions of CHOP and caspase-12. Results： Compared with group A and group B, the volume of cerebral infarction and mRNA expressions of CHOP and caspase-12 in group C, group D and group E were increased, with statistical significances （P〈0.05 or P〈O.01）; compared with group C, the volume of cerebral infarction and mRNA expressions of CHOP and caspase-12 in group D and group E were decreased significantly （P〈0.05 or P〈0.01）; there were no significant differences between group D and group E in comparing the above items （P〉0.05）. Conclusion： EA at Neiguan （PC 6） and Baihui （GV 20） can effectively suppress the volume of cerebral infarction. Furthermore, the underlying mechanism of EA at Neiguan （PC 6） and Baihui （GV 20） is possibly related to the down-regulation of CHOP and caspase-12 mRNA expressions, so as to decrease cell apoptosis.展开更多
目的了解老年骨质疏松性椎体骨折患者衰弱及出院准备度现状,并探讨两者间的关系。方法采用便利抽样的方法,采用老年人衰弱评估量表(Chinese version of tiburg frailty indicator,CTFI)及出院准备度量表(readiness for hospital dischar...目的了解老年骨质疏松性椎体骨折患者衰弱及出院准备度现状,并探讨两者间的关系。方法采用便利抽样的方法,采用老年人衰弱评估量表(Chinese version of tiburg frailty indicator,CTFI)及出院准备度量表(readiness for hospital discharge scale,RHDS)对182例老年骨质疏松性椎体骨折患者进行问卷调查,并分析其相关性。结果老年OVCF患者衰弱得分为(15.74±5.37)分,54.2%的患者可诊断为衰弱;患者出院准备度得分为(66.71±15.63)分,以个人状态维度均分最低;患者衰弱与出院准备度呈负相关(r=-0.252,P<0.05)。结论老年骨质疏松性椎体骨折患者衰弱程度较严重,出院准备度较差,医护人员应尽力扭转患者衰弱的进程,以提高患者出院准备度,减少再入院风险。展开更多
基金supported by National Natural Science Foundation of China,No.81202771~~
文摘Objective： To investigate the effects of electroacupuncture （EA） at Neiguan （PC 6） and Baihui （GV 20） by observing the changes of CCAAT/enhancer-binding protein（C/EBP） homologous protein （CHOP） and caspase-12 gene expressions in rats after cerebral ischemia-reperfusion injury （IRI）, and explore whether the apoptosis pathway of endoplasmic reticulum stress （ERS） is involved in the protective mechanisms of EA. Methods： Sixty rats were randomly assigned to five groups （12 in each group）： a normal control group （group A）, a sham-operation group （group B）, an operation group （group C）, an Edaravone group （group D） and an EA group （group E）. The cerebral IRI rat model was induced by middle cerebral artery occlusion （MCAO） using intraluminal monofilament. 2,3,5-triphenyl tetrazolium chloride （TTC） staining was adopted in the measurement of cerebral infarction volume. Real-time polymerase chain reaction （RT-PCR） was used to determine the mRNA expressions of CHOP and caspase-12. Results： Compared with group A and group B, the volume of cerebral infarction and mRNA expressions of CHOP and caspase-12 in group C, group D and group E were increased, with statistical significances （P〈0.05 or P〈O.01）; compared with group C, the volume of cerebral infarction and mRNA expressions of CHOP and caspase-12 in group D and group E were decreased significantly （P〈0.05 or P〈0.01）; there were no significant differences between group D and group E in comparing the above items （P〉0.05）. Conclusion： EA at Neiguan （PC 6） and Baihui （GV 20） can effectively suppress the volume of cerebral infarction. Furthermore, the underlying mechanism of EA at Neiguan （PC 6） and Baihui （GV 20） is possibly related to the down-regulation of CHOP and caspase-12 mRNA expressions, so as to decrease cell apoptosis.
基金国家自然科学基金(81470130)；南华大学研究生科研创新项目(2016XCX41)Fund program:National Natural Science Foundation of China，Postgraduate Innovation Project of University of South China
基金国家自然科学基金面上项目(81272959)；南华大学研究生科研创新项目(2015XCX41)Fund program:National Natural Science Foundation of China，Graduate Student Scientific Research Foundation of University of South China
文摘目的了解老年骨质疏松性椎体骨折患者衰弱及出院准备度现状,并探讨两者间的关系。方法采用便利抽样的方法,采用老年人衰弱评估量表(Chinese version of tiburg frailty indicator,CTFI)及出院准备度量表(readiness for hospital discharge scale,RHDS)对182例老年骨质疏松性椎体骨折患者进行问卷调查,并分析其相关性。结果老年OVCF患者衰弱得分为(15.74±5.37)分,54.2%的患者可诊断为衰弱;患者出院准备度得分为(66.71±15.63)分,以个人状态维度均分最低;患者衰弱与出院准备度呈负相关(r=-0.252,P<0.05)。结论老年骨质疏松性椎体骨折患者衰弱程度较严重,出院准备度较差,医护人员应尽力扭转患者衰弱的进程,以提高患者出院准备度,减少再入院风险。
