Brassica chinensis L. were foliarly applied with glycinebetaine (GB), as this species is unable to synthesis GB and sensitive to osmotic stress such as salt. The exogenous GB was easily absorbed and transported by t...Brassica chinensis L. were foliarly applied with glycinebetaine (GB), as this species is unable to synthesis GB and sensitive to osmotic stress such as salt. The exogenous GB was easily absorbed and transported by the leaf of B. chinensis . Its application (0-20 mmol/L) enhanced the plant tolerance to salt stress. The treatment of 15 mmol/L GB significantly decreased the Na + accumulation in leaf and root under NaCl stress. This difference in accumulating Na + and K + is caused by higher selectivity of root absorption. Furthermore, GB increased H +_ATPase activity of root plasma membrane evidently. This result strongly suggested that in root the decreased Na + accumulation was caused by the GB accumulation that enhanced the extrusion of Na + from the cell in some way through plasma membrane transporter, e.g. Na +/H + antiport driven by H +_ATPase. The GB application was also found to stabilize the plasma membrane, to decrease the loss of chlorophyll, and to stimulate the osmosis induced proline response under salt stress.展开更多
目的本研究对糖尿病自我管理行为量表(Summary of Diabetes Self-Care Activities,SDSCA)和糖尿病自我管理信心量表(Confidence In Diabetes Self-care,CIDS)进行翻译、修订,并检验其信度和效度,为评估中国成人1型糖尿病患者自我管理水...目的本研究对糖尿病自我管理行为量表(Summary of Diabetes Self-Care Activities,SDSCA)和糖尿病自我管理信心量表(Confidence In Diabetes Self-care,CIDS)进行翻译、修订,并检验其信度和效度,为评估中国成人1型糖尿病患者自我管理水平提供有效应用工具。方法对SDSCA量表及CIDS量表进行翻译及修订,最终得到中文版量表C-SDSCA及C-CIDS。于2015年1月至2015年12月,从广东省1型糖尿病转化医学研究数据库中选取100名成人1型糖尿病患者用C-SDSCA及C-CIDS进行问卷调查,并从中随机抽取15例患者在4周后进行第2次问卷调查。用克隆巴赫α系数(Cronbach′sα)评估量表的信度,因子分析法评估其效度。分别计算两个量表的得分,并用Spearman相关系数分析两个量表得分之间的相关性。结果C-SDSCA信度检测结果:整体的Cronbach′sα为0.72,量表的重测信度是0.95(分量表0.67~1.00);效度检测:采用因子分析法,共提取4个公因子,累计贡献率是87.39%。同样地,C-CIDS信度检测:整体的Cronbach′sα为0.84,重测信度是0.70(分量表0.49~0.86);效度检测:共提取6个公因子,累计贡献率为75.41%。C-CIDS得分为70.50±14.75,C-SDSCA得分为33.50±19.75,两个量表得分之间成正相关(r=0.61,P<0.01)。结论修订后的C-SDSCA和C-CIDS量表均有良好的信度和效度,可作为评价中国成人1型糖尿病患者自我管理效能和自我管理行为的工具。展开更多
文摘Brassica chinensis L. were foliarly applied with glycinebetaine (GB), as this species is unable to synthesis GB and sensitive to osmotic stress such as salt. The exogenous GB was easily absorbed and transported by the leaf of B. chinensis . Its application (0-20 mmol/L) enhanced the plant tolerance to salt stress. The treatment of 15 mmol/L GB significantly decreased the Na + accumulation in leaf and root under NaCl stress. This difference in accumulating Na + and K + is caused by higher selectivity of root absorption. Furthermore, GB increased H +_ATPase activity of root plasma membrane evidently. This result strongly suggested that in root the decreased Na + accumulation was caused by the GB accumulation that enhanced the extrusion of Na + from the cell in some way through plasma membrane transporter, e.g. Na +/H + antiport driven by H +_ATPase. The GB application was also found to stabilize the plasma membrane, to decrease the loss of chlorophyll, and to stimulate the osmosis induced proline response under salt stress.
文摘目的本研究对糖尿病自我管理行为量表(Summary of Diabetes Self-Care Activities,SDSCA)和糖尿病自我管理信心量表(Confidence In Diabetes Self-care,CIDS)进行翻译、修订,并检验其信度和效度,为评估中国成人1型糖尿病患者自我管理水平提供有效应用工具。方法对SDSCA量表及CIDS量表进行翻译及修订,最终得到中文版量表C-SDSCA及C-CIDS。于2015年1月至2015年12月,从广东省1型糖尿病转化医学研究数据库中选取100名成人1型糖尿病患者用C-SDSCA及C-CIDS进行问卷调查,并从中随机抽取15例患者在4周后进行第2次问卷调查。用克隆巴赫α系数(Cronbach′sα)评估量表的信度,因子分析法评估其效度。分别计算两个量表的得分,并用Spearman相关系数分析两个量表得分之间的相关性。结果C-SDSCA信度检测结果:整体的Cronbach′sα为0.72,量表的重测信度是0.95(分量表0.67~1.00);效度检测:采用因子分析法,共提取4个公因子,累计贡献率是87.39%。同样地,C-CIDS信度检测:整体的Cronbach′sα为0.84,重测信度是0.70(分量表0.49~0.86);效度检测:共提取6个公因子,累计贡献率为75.41%。C-CIDS得分为70.50±14.75,C-SDSCA得分为33.50±19.75,两个量表得分之间成正相关(r=0.61,P<0.01)。结论修订后的C-SDSCA和C-CIDS量表均有良好的信度和效度,可作为评价中国成人1型糖尿病患者自我管理效能和自我管理行为的工具。
