Objective： To investigate the effects and mechanism of qi-tonifying and stasiseliminating （QTSE） therapy on the expression of vascular endothelial growth factor （VEGF） and its receptors Fit-1 and FIk-1 in the bra...Objective： To investigate the effects and mechanism of qi-tonifying and stasiseliminating （QTSE） therapy on the expression of vascular endothelial growth factor （VEGF） and its receptors Fit-1 and FIk-1 in the brains of intracerebral hemorrhagic （model） rats. Methods： One hundred and eighty Sprague-Dawley rats were randomly divided into six groups： the normal group （n=5）, the sham-operative （SO） group （n=35）, the model group （n=35）, the QTSE group （n=35）, the QT group （n=35） and the SE group （n=35）. All the rats except those in the normal group and SO group were established into an intracerebral hemorrhage（ICH） model by intracerebral injection of collagenase type Ⅶ and the latter three were orally administered with Buyang Huanwu Decoction （补阳还五汤, a classical recipe for QTSE） or with some of its components for qi-tonification and for stasis-elimination, respectively. To the other three groups, normal saline solutions were given instead. Behavioral tests were carried out in the animals randomly chosen from each group on days 1, 2, 4, 7, 14, 21 and 28 after modeling. The expressions of VEGF, FIk-1 and Fit-1 were determined by immunohistochemistry and the number of vascular segments with positive expression in the injured brain area of the rats was calculated. Results： From day 7 onwards, the asymmetric forelimb use rate in the QTSE group recovered more significantly than that in the other model groups. In the model group, the expressions of VEGF, FIk-1 and Fit-1 appeared on day 1 and reached a peak on day 21, then weakened gradually. In the QTSE group, as compared with the other model groups, a higher level of VEGF expression was shown from day 7 （P〈0.01） and a higher level of Fit-1 expression was shown from the 7th day to the 21st day （P〈0.01）. Conclusion： QTSE therapy can up-regulate the expressions of VEGF and its receptors （FIk-1 and Fit-l） and improve the recovery of kinetic function in the ICH rats, which may be correlated wit展开更多
目的:观察与微血管系统重建过程密切相关的促血管生成素(Angiopoietin-1,Ang-1)及其受体含免疫球蛋白样环和上皮生长因子样域酪氨酸激酶-2(tyrosine kinase that contains immunoglobulin-like loops and epidermal growth factor-simil...目的:观察与微血管系统重建过程密切相关的促血管生成素(Angiopoietin-1,Ang-1)及其受体含免疫球蛋白样环和上皮生长因子样域酪氨酸激酶-2(tyrosine kinase that contains immunoglobulin-like loops and epidermal growth factor-similar domains-2,Tie-2)在大鼠基底核脑出血后表达的动态变化。方法:用Ⅶ型胶原酶诱导大鼠脑出血模型,采用HE染色观察大鼠脑组织形态学改变,免疫组化法检测第1、2、4、7、14、21和28d血管生成素Ang-1和其受体Tie-2的表达,计数阳性血管作为观察指标。结果:HE染色显示正常组及假手术组各时间点取材未见血肿及局部明显病理学改变,而模型组第4d血肿周围出现微血管段,而后阳性微血管段表达逐渐持续增多,至第21d大量伸入血肿区;免疫组化研究显示正常及假手术组不同时间点Ang-1和Tie-2表达均未见明显变化,模型组大鼠在脑出血后第2d起Ang-1和Tie-2阳性微血管表达明显多于其他两组,而后表达逐渐升高(P<0.01),至21d达到高峰,随后开始下降,28d时仍有表达。结论:在脑出血后,损伤区Ang-1及其受体Tie-2的表达上调,可能通过调节血管生成过程而促进脑出血损伤区微血管系统重建。展开更多
基金Supported by the National Natural Science Foundation of China(No.30400581)Chinese Post-doctoral Science Foundation(No.2005038224)Hunan Provincial Key Young Teachers in Universities and Hunan Provincial Funds for Key Subjects
文摘Objective： To investigate the effects and mechanism of qi-tonifying and stasiseliminating （QTSE） therapy on the expression of vascular endothelial growth factor （VEGF） and its receptors Fit-1 and FIk-1 in the brains of intracerebral hemorrhagic （model） rats. Methods： One hundred and eighty Sprague-Dawley rats were randomly divided into six groups： the normal group （n=5）, the sham-operative （SO） group （n=35）, the model group （n=35）, the QTSE group （n=35）, the QT group （n=35） and the SE group （n=35）. All the rats except those in the normal group and SO group were established into an intracerebral hemorrhage（ICH） model by intracerebral injection of collagenase type Ⅶ and the latter three were orally administered with Buyang Huanwu Decoction （补阳还五汤, a classical recipe for QTSE） or with some of its components for qi-tonification and for stasis-elimination, respectively. To the other three groups, normal saline solutions were given instead. Behavioral tests were carried out in the animals randomly chosen from each group on days 1, 2, 4, 7, 14, 21 and 28 after modeling. The expressions of VEGF, FIk-1 and Fit-1 were determined by immunohistochemistry and the number of vascular segments with positive expression in the injured brain area of the rats was calculated. Results： From day 7 onwards, the asymmetric forelimb use rate in the QTSE group recovered more significantly than that in the other model groups. In the model group, the expressions of VEGF, FIk-1 and Fit-1 appeared on day 1 and reached a peak on day 21, then weakened gradually. In the QTSE group, as compared with the other model groups, a higher level of VEGF expression was shown from day 7 （P〈0.01） and a higher level of Fit-1 expression was shown from the 7th day to the 21st day （P〈0.01）. Conclusion： QTSE therapy can up-regulate the expressions of VEGF and its receptors （FIk-1 and Fit-l） and improve the recovery of kinetic function in the ICH rats, which may be correlated wit
