A new type of sol-gel/organic hybrid composite film based on chitosan(CS) with methytrimethoxysilane(MTOS) was developed for the fabrication of an amperometric glucose biosensor. The hybrid composite film was used to ...A new type of sol-gel/organic hybrid composite film based on chitosan(CS) with methytrimethoxysilane(MTOS) was developed for the fabrication of an amperometric glucose biosensor. The hybrid composite film was used to immobilize glucose oxidase(GOD) on the surface of Prussian Blue(PB)-modified glass carbon electrode. Effects of some experimental conditions such as pH, temperature and applied potential etc. on the current response of the biosensor were investigated. The biosensor has a fast response less than 10 s and linear calibration range from 5.0×10 -6 to 2.4×10 -3 mol/L with detection limit of 1.0×10 -6 mol/L. The apparent Michaelis-Menten constant K m is found to be 3.2×10 -3 mol/L. The activation energy for enzymatic reaction is calculated to be 21.9 kJ/mol. The biosensor has a high sensitivity(420 μA·mol -1·L), long-term stability and good selectivity. This method has been used to determine the content of glucose in the real blood samples.展开更多
A method based on capillary electrophoresis with a high frequency conductivity detection was developed for the determination of Sinomenine. The key factors for separation and determination were studied and the best an...A method based on capillary electrophoresis with a high frequency conductivity detection was developed for the determination of Sinomenine. The key factors for separation and determination were studied and the best analysis conditions were obtained. In the buffer of 2.0 mmol/L HAc-3.0 mmol/L NaAc-10.0% CH 3OH at a separation voltage of 22 kV, Sinomenine in the sample could be separated and detected within 4 min. The linear mass concentration of Sinomenine is in the range of 1.0—36.0 μg/mL, the limit of detection reached 0.20 μg/mL. The method was used for the analysis of Sinomenine in Sinomenium acutum and tablets satisfactorily with a recovery of 96%—102%.展开更多
文摘A new type of sol-gel/organic hybrid composite film based on chitosan(CS) with methytrimethoxysilane(MTOS) was developed for the fabrication of an amperometric glucose biosensor. The hybrid composite film was used to immobilize glucose oxidase(GOD) on the surface of Prussian Blue(PB)-modified glass carbon electrode. Effects of some experimental conditions such as pH, temperature and applied potential etc. on the current response of the biosensor were investigated. The biosensor has a fast response less than 10 s and linear calibration range from 5.0×10 -6 to 2.4×10 -3 mol/L with detection limit of 1.0×10 -6 mol/L. The apparent Michaelis-Menten constant K m is found to be 3.2×10 -3 mol/L. The activation energy for enzymatic reaction is calculated to be 21.9 kJ/mol. The biosensor has a high sensitivity(420 μA·mol -1·L), long-term stability and good selectivity. This method has been used to determine the content of glucose in the real blood samples.
文摘A method based on capillary electrophoresis with a high frequency conductivity detection was developed for the determination of Sinomenine. The key factors for separation and determination were studied and the best analysis conditions were obtained. In the buffer of 2.0 mmol/L HAc-3.0 mmol/L NaAc-10.0% CH 3OH at a separation voltage of 22 kV, Sinomenine in the sample could be separated and detected within 4 min. The linear mass concentration of Sinomenine is in the range of 1.0—36.0 μg/mL, the limit of detection reached 0.20 μg/mL. The method was used for the analysis of Sinomenine in Sinomenium acutum and tablets satisfactorily with a recovery of 96%—102%.
