目的:在学龄前儿童父母群体中检验中文版童年不良经历(adverse childhood experiences international questionnaire,ACE-IQ)问卷的信度和效度。方法:通过分层随机整群抽样,使用经跨文化调试得到的中文版ACE-IQ在北京市通州区6所幼儿园...目的:在学龄前儿童父母群体中检验中文版童年不良经历(adverse childhood experiences international questionnaire,ACE-IQ)问卷的信度和效度。方法:通过分层随机整群抽样,使用经跨文化调试得到的中文版ACE-IQ在北京市通州区6所幼儿园的学龄前儿童父母中开展调查,将调查数据随机分为两部分:一部分数据(n=602)用于探索性因子分析,进行条目筛选和结构效度评价,形成最终的中文版ACE-IQ;另一部分数据(n=700)用来对最终形成的中文版ACE-IQ进行验证性因子分析、效标效度分析以及信度分析。同时,采用专家咨询法,对最终的中文版ACE-IQ进行内容效度评价。结果:在删除集体暴力的4个条目后,25个条目的中文版ACE-IQ呈现七因子结构,即情感忽视、身体忽视、家庭功能不全、家庭暴力、身心虐待、性虐待和家庭外暴力。中文版ACE-IQ二元评分法总分与儿童期创伤问卷精简版(childhood trauma questionaire-28 item short form,CTQ-SF,r=0.354,P<0.001)和流调中心抑郁量表(the center for epidemiological studies depression scale,CES-D,r=0.313,P<0.001)的总分均成正相关。中文版ACE-IQ内容效度指数均为0.80~1.00,量表平均内容效度为0.984,中文版ACE-IQ具有良好的结构效度、效标效度和内容效度。总量表的内部一致性Cronbach’sα系数为0.818,分半信度Spearman-Brown系数为0.621,具有良好的信度。结论:形成了25个条目、7个维度的中文版ACE-IQ,在中国学龄前儿童父母中具有良好的信度和效度,可用于中国文化背景下学龄前儿童父母群体中童年不良经历的最低阈值评价。展开更多
Combined administration of fluticasone propionate and salmeterol xinofoate has been widely used for the treatment of asthma in recent decades. In this investigation, we developed and validated a novel and sensitive ul...Combined administration of fluticasone propionate and salmeterol xinofoate has been widely used for the treatment of asthma in recent decades. In this investigation, we developed and validated a novel and sensitive ultra performance liquid chromatography-tandem mass spectrometry （UPLC-MS/MS） method for simultaneous determination of fluticasone propionate and salmeterol xinofoate in human plasma. Following a simple SPE sample extraction in 96-well plate format, chromatography was performed on a Waters ACQUITY UPLC BEH C 18 column （1.7 μm, 50 min×2.1 mm） with mobile phase consisting of 100% MeOH and 0.1% NH4OH in water on a gradient program at flow rate of 0.5 mL/min. Detection of analytes and internal standards was accomplished using multiple reaction monitoring （MRM） of precursor〉product ion pairs of m/z 501.4〉313.2 （fluticasone propionate）, 506.4〉293.3 （fluticasone propionate-d5）, 416.4〉232.1 （salmeterol xinofoate） and 419.3〉235.2 （salmeterol-d3）. The assay range was 2.50-500 pg/mL for both analytes, and a 1/x2 weighted linear regression model was used. The inter-assay accuracy and precision of the method were within ±8.6%. The recoveries from 0.30 mL of plasma were greater than 51.0% and 54.6% for fluticasone propionate and salmeterol, respectively, and the results were consistent across low, middle and high concentration levels. The method was validated following FDA, EMA and CFDA （China Food and Drug Administration）＇s guidance on bioanalysis and then successfully applied to support a clinical study in healthy Chinese subjects following inhaled administration of a single combination of fluticasone propionate/salmeterol （250 μg/50 μg）.展开更多
文摘目的对中国2011年脊髓灰质炎(脊灰)实验室网络(Polio Laboratories Network,PLN)监测数据(未包括香港、澳门特别行政区和台湾地区,下同)进行统计分析,为应对新疆维吾尔自治区(新疆)输入Ⅰ型脊灰野病毒(Tepe1 Wild Poliovirus,WPVⅠ)疫情和维持中国无脊灰提供科学支撑。方法分析中国2011年免疫规划监测信息管理系统数据库中,31个省(自治区、直辖市,下同)报告的急性弛缓性麻痹(Acute Flaccid Paralysis,AFP)病例个案调查表和中国疾病预防控制中心(Center for Disease Control and Prevention,CDC)病毒病预防控制所国家脊灰实验室(National Polio Laboratory,NPL)的监测数据库,评价PLN的各项运转指标。结果中国2011年PLN共收集了6205例AFP病例的12 253份粪便标本,合格粪便标本采集率为90.9%。从6089例AFP病例中分离到脊灰病毒(Poliovirus,PV)的207例,分离率为3.4%;分离到非脊灰肠道病毒(Non-polio Enterovirus,NPEV)的782例,分离率为12.8%。NPL收到中国PLN送检的PV 594株,鉴定结果:9例为疫苗衍生(Vaccine-derived)PV(VDPV),其中Ⅰ型1例,Ⅱ型7例,Ⅲ型1例;53例为WPVⅠ,21例来源于WPV病例,30例来源于AFP病例接触者或健康人群。2份环境污水来源于新疆和田地区环境监测。2011年NPL和31个省级CDC脊灰实验室通过了世界卫生组织(World Health Organization,WHO)PV分离与鉴定的盲样标本考核;NPL还通过了PV荧光定量聚合酶链反应型内鉴定和PV核苷酸序列测定和分析的盲样考核;NPL和13个省级CDC脊灰实验室接受并通过了WHO的现场认证考核。结论中国2011年PLN维持着高水平的运转,及时发现和鉴定了VDPV病例、新疆输入WPVⅠ和局部爆发疫情,为卫生部制订应对WPV和VDPV的免疫策略,为阻断WPV和VDPV的传播提供了重要的科学依据。
文摘目的:在学龄前儿童父母群体中检验中文版童年不良经历(adverse childhood experiences international questionnaire,ACE-IQ)问卷的信度和效度。方法:通过分层随机整群抽样,使用经跨文化调试得到的中文版ACE-IQ在北京市通州区6所幼儿园的学龄前儿童父母中开展调查,将调查数据随机分为两部分:一部分数据(n=602)用于探索性因子分析,进行条目筛选和结构效度评价,形成最终的中文版ACE-IQ;另一部分数据(n=700)用来对最终形成的中文版ACE-IQ进行验证性因子分析、效标效度分析以及信度分析。同时,采用专家咨询法,对最终的中文版ACE-IQ进行内容效度评价。结果:在删除集体暴力的4个条目后,25个条目的中文版ACE-IQ呈现七因子结构,即情感忽视、身体忽视、家庭功能不全、家庭暴力、身心虐待、性虐待和家庭外暴力。中文版ACE-IQ二元评分法总分与儿童期创伤问卷精简版(childhood trauma questionaire-28 item short form,CTQ-SF,r=0.354,P<0.001)和流调中心抑郁量表(the center for epidemiological studies depression scale,CES-D,r=0.313,P<0.001)的总分均成正相关。中文版ACE-IQ内容效度指数均为0.80~1.00,量表平均内容效度为0.984,中文版ACE-IQ具有良好的结构效度、效标效度和内容效度。总量表的内部一致性Cronbach’sα系数为0.818,分半信度Spearman-Brown系数为0.621,具有良好的信度。结论:形成了25个条目、7个维度的中文版ACE-IQ,在中国学龄前儿童父母中具有良好的信度和效度,可用于中国文化背景下学龄前儿童父母群体中童年不良经历的最低阈值评价。
文摘Combined administration of fluticasone propionate and salmeterol xinofoate has been widely used for the treatment of asthma in recent decades. In this investigation, we developed and validated a novel and sensitive ultra performance liquid chromatography-tandem mass spectrometry （UPLC-MS/MS） method for simultaneous determination of fluticasone propionate and salmeterol xinofoate in human plasma. Following a simple SPE sample extraction in 96-well plate format, chromatography was performed on a Waters ACQUITY UPLC BEH C 18 column （1.7 μm, 50 min×2.1 mm） with mobile phase consisting of 100% MeOH and 0.1% NH4OH in water on a gradient program at flow rate of 0.5 mL/min. Detection of analytes and internal standards was accomplished using multiple reaction monitoring （MRM） of precursor〉product ion pairs of m/z 501.4〉313.2 （fluticasone propionate）, 506.4〉293.3 （fluticasone propionate-d5）, 416.4〉232.1 （salmeterol xinofoate） and 419.3〉235.2 （salmeterol-d3）. The assay range was 2.50-500 pg/mL for both analytes, and a 1/x2 weighted linear regression model was used. The inter-assay accuracy and precision of the method were within ±8.6%. The recoveries from 0.30 mL of plasma were greater than 51.0% and 54.6% for fluticasone propionate and salmeterol, respectively, and the results were consistent across low, middle and high concentration levels. The method was validated following FDA, EMA and CFDA （China Food and Drug Administration）＇s guidance on bioanalysis and then successfully applied to support a clinical study in healthy Chinese subjects following inhaled administration of a single combination of fluticasone propionate/salmeterol （250 μg/50 μg）.
