Two brown spot disease resistant varieties of tobacco, named NC89 V1 and NC89 V2, were obtained by first inducing from disease sensitive NC89 with the two hypovirulent viruses ToMV N14 and CMV SV52, then tissue ...Two brown spot disease resistant varieties of tobacco, named NC89 V1 and NC89 V2, were obtained by first inducing from disease sensitive NC89 with the two hypovirulent viruses ToMV N14 and CMV SV52, then tissue culturing the plants and finally seed breeding. The disease resistance for both varieties was verified to be stable in three generations. The transcription activities of five plant defense response genes, pr 1a, chi, chs, pal, and lox, in NC89, NC89 V1, and NC89 V2 were studied through RNA blot hybridization. Genome DNA structural differences among the three tobacco lines were identified using randomly amplified polymorphic DNA(RAPD).展开更多
Glucose transporter (GT), molecular weight 55 kd, is an important biological functional membrane protein, by which glucose is transported into the cell from the extracellular environment and used as a source of energy.
文摘Two brown spot disease resistant varieties of tobacco, named NC89 V1 and NC89 V2, were obtained by first inducing from disease sensitive NC89 with the two hypovirulent viruses ToMV N14 and CMV SV52, then tissue culturing the plants and finally seed breeding. The disease resistance for both varieties was verified to be stable in three generations. The transcription activities of five plant defense response genes, pr 1a, chi, chs, pal, and lox, in NC89, NC89 V1, and NC89 V2 were studied through RNA blot hybridization. Genome DNA structural differences among the three tobacco lines were identified using randomly amplified polymorphic DNA(RAPD).
文摘Glucose transporter (GT), molecular weight 55 kd, is an important biological functional membrane protein, by which glucose is transported into the cell from the extracellular environment and used as a source of energy.
