目的研究维生素E琥珀酸酯(vitamin E succinate,VES)对体外培养的人晶状体上皮细胞(HLECs)增殖的影响。方法人晶状体上皮细胞株HLECs-B3以VES刺激24h、48h、72h、96h,VES浓度为5μg/ml、10μg/ml和20μg/ml,用MTT比色法测定VES对细胞增...目的研究维生素E琥珀酸酯(vitamin E succinate,VES)对体外培养的人晶状体上皮细胞(HLECs)增殖的影响。方法人晶状体上皮细胞株HLECs-B3以VES刺激24h、48h、72h、96h,VES浓度为5μg/ml、10μg/ml和20μg/ml,用MTT比色法测定VES对细胞增殖的抑制作用;以流式细胞仪分析细胞周期。结果VES对人晶状体上皮细胞的增殖具有显著的抑制作用,表现为时间和剂量依赖关系。并且将细胞周期阻滞于G0/G1期。结论VES对体外培养的人晶状体上皮细胞的增殖具有抑制作用,为临床筛选防治后发性白内障的药物提供依据。展开更多
目的总结分析儿童虹膜夹持型人工晶状体植入术后眼压、房角和角膜内皮的变化,探讨临床应用的有效性和安全性。方法 21例(30只眼)患者中,钝挫伤晶状体脱位2例,马-凡综合征晶状体脱位8例,外伤性白内障术后无晶状体眼4例,先天性白内障术后...目的总结分析儿童虹膜夹持型人工晶状体植入术后眼压、房角和角膜内皮的变化,探讨临床应用的有效性和安全性。方法 21例(30只眼)患者中,钝挫伤晶状体脱位2例,马-凡综合征晶状体脱位8例,外伤性白内障术后无晶状体眼4例,先天性白内障术后无晶状体眼7例。均无后囊支撑,无法行后房人工晶状体植入而应用虹膜夹持型人工晶状体植入术。术后观察视力、眼压、角膜内皮计数、房角改变等。结果随访3～12月(平均6个月),术后视力0.1～1.0,均较术前明显提高;眼压正常11～20 mm Hg,无继发性青光眼;角膜内皮细胞计数无明显变化;超声生物显微镜房角检查观察房角无明显改变。结论本手术时间短、操作简单,在不能常规植入后房型人工晶状体的特殊情况下选择虹膜夹持型人工晶状体植入术是安全有效的,无明显并发症发生,尤其是角膜内皮细胞计数无显著衰竭,对房角和玻璃体无干扰,是其最大优点。展开更多
Three plasmids （pGenesil-P1, pGenesil-P2, pGenesil-P3） with different p27Kip1-shRNA sequences were designed and synthesized. Their effects on the proliferation of bovine corneal endothelial cells （bCEC） were inves...Three plasmids （pGenesil-P1, pGenesil-P2, pGenesil-P3） with different p27Kip1-shRNA sequences were designed and synthesized. Their effects on the proliferation of bovine corneal endothelial cells （bCEC） were investigated. Plasmid expressing irrelevant shRNA with a random combination was used as negative control （pGenesil-HK）. The recombination of four plamids was confirmed by restrictive enzyme digestion and sequence analysis. The expression of mRNA and protein of p27Kip1 was detected by RT-PCR and Western blotting after stable transfection. The expressions of p27Kip1 mRNA and p27Kip1 protein of pGenesil-P1 group, pGenesil-P2 group and pGenesil-P3 group were all lower than those in the pGenesil-HK group and the blank group （non-transfected group）, pGenesil-P3 had the strongest inhibitory effect and was selected for the next steps. The proliferation rates of the pGenesil-P3 group, the pGenesil-HK group and the blank group were assessed by MTT. The influence of shRNA-p27Kip1 on bCEC cell cycle was detected by flow cytometry （FCM）. Compared with the control groups, the proliferation rate of the pGenesil-P3 group was increased significantly, and the ratio of S-phase also increased. It is concluded that shRNA-p27Kip1 could down-regulate the expression of p27Kip1 effectively and increase the proliferation of bCEC. RNA interference （RNAi） may be an effective means to promote the proliferation of CEC.展开更多
文摘目的研究维生素E琥珀酸酯(vitamin E succinate,VES)对体外培养的人晶状体上皮细胞(HLECs)增殖的影响。方法人晶状体上皮细胞株HLECs-B3以VES刺激24h、48h、72h、96h,VES浓度为5μg/ml、10μg/ml和20μg/ml,用MTT比色法测定VES对细胞增殖的抑制作用;以流式细胞仪分析细胞周期。结果VES对人晶状体上皮细胞的增殖具有显著的抑制作用,表现为时间和剂量依赖关系。并且将细胞周期阻滞于G0/G1期。结论VES对体外培养的人晶状体上皮细胞的增殖具有抑制作用,为临床筛选防治后发性白内障的药物提供依据。
文摘目的总结分析儿童虹膜夹持型人工晶状体植入术后眼压、房角和角膜内皮的变化,探讨临床应用的有效性和安全性。方法 21例(30只眼)患者中,钝挫伤晶状体脱位2例,马-凡综合征晶状体脱位8例,外伤性白内障术后无晶状体眼4例,先天性白内障术后无晶状体眼7例。均无后囊支撑,无法行后房人工晶状体植入而应用虹膜夹持型人工晶状体植入术。术后观察视力、眼压、角膜内皮计数、房角改变等。结果随访3～12月(平均6个月),术后视力0.1～1.0,均较术前明显提高;眼压正常11～20 mm Hg,无继发性青光眼;角膜内皮细胞计数无明显变化;超声生物显微镜房角检查观察房角无明显改变。结论本手术时间短、操作简单,在不能常规植入后房型人工晶状体的特殊情况下选择虹膜夹持型人工晶状体植入术是安全有效的,无明显并发症发生,尤其是角膜内皮细胞计数无显著衰竭,对房角和玻璃体无干扰,是其最大优点。
基金a grant from Hubei Provincial Natural Sciences Foundation of China (No. 2004ABA250)
文摘Three plasmids （pGenesil-P1, pGenesil-P2, pGenesil-P3） with different p27Kip1-shRNA sequences were designed and synthesized. Their effects on the proliferation of bovine corneal endothelial cells （bCEC） were investigated. Plasmid expressing irrelevant shRNA with a random combination was used as negative control （pGenesil-HK）. The recombination of four plamids was confirmed by restrictive enzyme digestion and sequence analysis. The expression of mRNA and protein of p27Kip1 was detected by RT-PCR and Western blotting after stable transfection. The expressions of p27Kip1 mRNA and p27Kip1 protein of pGenesil-P1 group, pGenesil-P2 group and pGenesil-P3 group were all lower than those in the pGenesil-HK group and the blank group （non-transfected group）, pGenesil-P3 had the strongest inhibitory effect and was selected for the next steps. The proliferation rates of the pGenesil-P3 group, the pGenesil-HK group and the blank group were assessed by MTT. The influence of shRNA-p27Kip1 on bCEC cell cycle was detected by flow cytometry （FCM）. Compared with the control groups, the proliferation rate of the pGenesil-P3 group was increased significantly, and the ratio of S-phase also increased. It is concluded that shRNA-p27Kip1 could down-regulate the expression of p27Kip1 effectively and increase the proliferation of bCEC. RNA interference （RNAi） may be an effective means to promote the proliferation of CEC.
