A series of affinity media were synthesized by using the oligohistines, His Gly, (His) 3 Gly and (His) 5 Gly as the ligands and the home made monosized, non porous copolymer(CL PGMA) beads containing active epoxide gr...A series of affinity media were synthesized by using the oligohistines, His Gly, (His) 3 Gly and (His) 5 Gly as the ligands and the home made monosized, non porous copolymer(CL PGMA) beads containing active epoxide groups as the support. The affinity chromatographic behavior between IgG and the oligohistines was investigated at pH 3 0, 4 5, 7 0 and 10 0. It showed the specific interaction at pH 4 5. The dissociation constants of IgG and oligohistine media(1H, 3H, 5H) were 3 037×10 -6 , 2 459×10 -6 and 1 905×10 -6 L/mol, respectively. The dissociation constants decreased with increasing the number of histine residue in oligopeptide chains. [WT5HZ]展开更多
Five guanido packings of affinity chromatography for the separation of urokinase have been synthesized. The separation characteristics and the effect of matrix and ligands on them have been studied. The guanidine deri...Five guanido packings of affinity chromatography for the separation of urokinase have been synthesized. The separation characteristics and the effect of matrix and ligands on them have been studied. The guanidine derivative is a fine ligand, the packings with ligand of arginine are much better than that with ligand of p-ABZ. The polyepoxypropyl methacrylate microsphere is a good matrix. The comprehensive function of packings with ligand of arginine and matrix of polyepoxypropyl methacrylate microsphere is more excellent than Sepharose-p-ABZ.展开更多
High performance GPC has been promoted by the development of porous microspheric packings. In modern GPC, two kinds of column packings are generally used, one based on porous silica and the other on porous polymer mic...High performance GPC has been promoted by the development of porous microspheric packings. In modern GPC, two kinds of column packings are generally used, one based on porous silica and the other on porous polymer microspheres. An attempt has been made to study preliminarily the effect of the internal structure of the basie material on the steric exclusion chromatographic process. On the basis of studying the re-展开更多
Commercially the catalytic carbonylation of methanol to acetic acid is carried out homogeneously. This process inherits the difficulty of homogeneous process in isolating the product and recycling the catalytic soluti...Commercially the catalytic carbonylation of methanol to acetic acid is carried out homogeneously. This process inherits the difficulty of homogeneous process in isolating the product and recycling the catalytic solution, not mentioning the trouble in handling the unstable catalytic rhodium earbonyl species. Heterogenisation of the homogeneous catalyst would provide the way to overcome these difficulties. However, this modification in general results in catalysts with lower activity and unavoidable展开更多
High performance IC as a new technique for determination of trace ions has been rapidly developed in recent years. The appearance of anion-exchange type column packings as the core of HPIC has provided an effective me...High performance IC as a new technique for determination of trace ions has been rapidly developed in recent years. The appearance of anion-exchange type column packings as the core of HPIC has provided an effective method for determination of trace anions which are difficult to detect by common analysis techniques. So展开更多
文摘A series of affinity media were synthesized by using the oligohistines, His Gly, (His) 3 Gly and (His) 5 Gly as the ligands and the home made monosized, non porous copolymer(CL PGMA) beads containing active epoxide groups as the support. The affinity chromatographic behavior between IgG and the oligohistines was investigated at pH 3 0, 4 5, 7 0 and 10 0. It showed the specific interaction at pH 4 5. The dissociation constants of IgG and oligohistine media(1H, 3H, 5H) were 3 037×10 -6 , 2 459×10 -6 and 1 905×10 -6 L/mol, respectively. The dissociation constants decreased with increasing the number of histine residue in oligopeptide chains. [WT5HZ]
文摘Five guanido packings of affinity chromatography for the separation of urokinase have been synthesized. The separation characteristics and the effect of matrix and ligands on them have been studied. The guanidine derivative is a fine ligand, the packings with ligand of arginine are much better than that with ligand of p-ABZ. The polyepoxypropyl methacrylate microsphere is a good matrix. The comprehensive function of packings with ligand of arginine and matrix of polyepoxypropyl methacrylate microsphere is more excellent than Sepharose-p-ABZ.
文摘High performance GPC has been promoted by the development of porous microspheric packings. In modern GPC, two kinds of column packings are generally used, one based on porous silica and the other on porous polymer microspheres. An attempt has been made to study preliminarily the effect of the internal structure of the basie material on the steric exclusion chromatographic process. On the basis of studying the re-
文摘Commercially the catalytic carbonylation of methanol to acetic acid is carried out homogeneously. This process inherits the difficulty of homogeneous process in isolating the product and recycling the catalytic solution, not mentioning the trouble in handling the unstable catalytic rhodium earbonyl species. Heterogenisation of the homogeneous catalyst would provide the way to overcome these difficulties. However, this modification in general results in catalysts with lower activity and unavoidable
基金Project supported by the National Natural Science Foundation of China
文摘High performance IC as a new technique for determination of trace ions has been rapidly developed in recent years. The appearance of anion-exchange type column packings as the core of HPIC has provided an effective method for determination of trace anions which are difficult to detect by common analysis techniques. So
