[Objective]The aim was to study the biological characteristics of Her2 over-expressed transgenic mice and to detect the changes in expression of KLF4. [Method] On the basis of establishment of Her2 over-expressed tran...[Objective]The aim was to study the biological characteristics of Her2 over-expressed transgenic mice and to detect the changes in expression of KLF4. [Method] On the basis of establishment of Her2 over-expressed transgenic mice, the population was expanded by propagation. The genotype of the offspring was identified by PCR, the body weight-age curve was drawn, and the expression of KLF4 （FL） and KLF4α was detected by RT-PCR. [Result] In the Her2-overex-pressed breast cancer-positive mice, the expression level of KLF4α decreased slightly in the course of breast cancer, and the expression level of KLF4 （FL） was increased significantly compared with those in normal mice. However, the expression level of KLF4 gene in the breast cancer-positive mice showed a downward trend. [Conclusion] This study will fill the gaps in the study of KLF4 at the animal model level and complete the study on relationship between KLF4 and the occurrence and development of breast cancer.展开更多
基金Supported by Project of Science-technology Department of Zhejiang Province(2017C37118)Key SRT Program of Jiaxing University(851716035)~~
文摘[Objective]The aim was to study the biological characteristics of Her2 over-expressed transgenic mice and to detect the changes in expression of KLF4. [Method] On the basis of establishment of Her2 over-expressed transgenic mice, the population was expanded by propagation. The genotype of the offspring was identified by PCR, the body weight-age curve was drawn, and the expression of KLF4 （FL） and KLF4α was detected by RT-PCR. [Result] In the Her2-overex-pressed breast cancer-positive mice, the expression level of KLF4α decreased slightly in the course of breast cancer, and the expression level of KLF4 （FL） was increased significantly compared with those in normal mice. However, the expression level of KLF4 gene in the breast cancer-positive mice showed a downward trend. [Conclusion] This study will fill the gaps in the study of KLF4 at the animal model level and complete the study on relationship between KLF4 and the occurrence and development of breast cancer.
