目的优选海带褐藻糖胶(FPS)磺化的工艺。方法以氯磺酸-N,N-二甲基甲酰胺(DMF)为磺化试剂,采用正交设计法,考察反应时间、反应温度及磺化剂用量对海带褐藻糖胶磺化反应的影响,找出制备高硫酸根含量海带褐藻糖胶的最佳方法。结果反应时间...目的优选海带褐藻糖胶(FPS)磺化的工艺。方法以氯磺酸-N,N-二甲基甲酰胺(DMF)为磺化试剂,采用正交设计法,考察反应时间、反应温度及磺化剂用量对海带褐藻糖胶磺化反应的影响,找出制备高硫酸根含量海带褐藻糖胶的最佳方法。结果反应时间和反应温度对海带褐藻糖胶的磺化有显著影响(0.01<P<0.05),磺化剂用量对海带褐藻糖胶的磺化有一定影响(0.05<P<0.1)。实验得到的海带褐藻糖胶的最佳磺化工艺为:反应时间3 h,反应温度50℃,磺化剂用量25 m l。结论用SO3-DMF作为磺化试剂对海带褐藻糖胶进行磺化是可行的,可避免使用毒性较强的SO3-吡啶作为磺化试剂,方法简单,可用于制备保健品和药品。展开更多
Studies on Gelidium amansii agar fractionations were carried out in this paper. Gelidium amansii agar was fractionated on DEAE-Cellulose, and four fractions were obtained sequentially. The fractions were analyzed on p...Studies on Gelidium amansii agar fractionations were carried out in this paper. Gelidium amansii agar was fractionated on DEAE-Cellulose, and four fractions were obtained sequentially. The fractions were analyzed on physical and chemical properties, and IR and 13C-NMR spectroscopy applied for elucidating the chemical structure. Among the four fractions obtained, water fraction measured up to the standard of low EEO agarose. The sulfate content, ash content, electroendosmosis and gel strength (1%) of water fraction were 0.16%, 0.34%, 0.12 and 1 130g/cm2 respectively, similar to those of the Sigma products.展开更多
文摘目的优选海带褐藻糖胶(FPS)磺化的工艺。方法以氯磺酸-N,N-二甲基甲酰胺(DMF)为磺化试剂,采用正交设计法,考察反应时间、反应温度及磺化剂用量对海带褐藻糖胶磺化反应的影响,找出制备高硫酸根含量海带褐藻糖胶的最佳方法。结果反应时间和反应温度对海带褐藻糖胶的磺化有显著影响(0.01<P<0.05),磺化剂用量对海带褐藻糖胶的磺化有一定影响(0.05<P<0.1)。实验得到的海带褐藻糖胶的最佳磺化工艺为:反应时间3 h,反应温度50℃,磺化剂用量25 m l。结论用SO3-DMF作为磺化试剂对海带褐藻糖胶进行磺化是可行的,可避免使用毒性较强的SO3-吡啶作为磺化试剂,方法简单,可用于制备保健品和药品。
基金the Innovative Key Project of the Chinese Academy of Sciences (KZCX2-YW-209)
文摘Studies on Gelidium amansii agar fractionations were carried out in this paper. Gelidium amansii agar was fractionated on DEAE-Cellulose, and four fractions were obtained sequentially. The fractions were analyzed on physical and chemical properties, and IR and 13C-NMR spectroscopy applied for elucidating the chemical structure. Among the four fractions obtained, water fraction measured up to the standard of low EEO agarose. The sulfate content, ash content, electroendosmosis and gel strength (1%) of water fraction were 0.16%, 0.34%, 0.12 and 1 130g/cm2 respectively, similar to those of the Sigma products.
