Polyamidoamine dendrimers using inositol as a core were designed, synthesized and characterized. The electrostatics interaction of dendrimer with DNA causes the condensation of DNA to form DNA/dendrimer complexes. The...Polyamidoamine dendrimers using inositol as a core were designed, synthesized and characterized. The electrostatics interaction of dendrimer with DNA causes the condensation of DNA to form DNA/dendrimer complexes. The sizes of complexes prepared from dendrimer G6 and pRE Luc at N/P ratio 10: 1 range from 80 to 300 nm. Dendrimers G3—G6 are able to efficiently deliver pRE Luc and pSV β-gal gene into HeLa and HEK 293 cells. The transfection efficiencies of G5 and G6 are much higher than that of poly L-lysine (PLL) and comparable to that of the commercial branched polyethylenimine (PEI, Mw 25000), whereas the cytoctoxicities of G5 and G6 are lower than that of PLL.展开更多
基金supported by the National Key Basic Research Program of China(973 Program)(Grant No.G1999064703)Specialized Research Fund for the Doctoral Program of Higher Education(Grant No.200048604)Creative Fund of Wuhan University
文摘Polyamidoamine dendrimers using inositol as a core were designed, synthesized and characterized. The electrostatics interaction of dendrimer with DNA causes the condensation of DNA to form DNA/dendrimer complexes. The sizes of complexes prepared from dendrimer G6 and pRE Luc at N/P ratio 10: 1 range from 80 to 300 nm. Dendrimers G3—G6 are able to efficiently deliver pRE Luc and pSV β-gal gene into HeLa and HEK 293 cells. The transfection efficiencies of G5 and G6 are much higher than that of poly L-lysine (PLL) and comparable to that of the commercial branched polyethylenimine (PEI, Mw 25000), whereas the cytoctoxicities of G5 and G6 are lower than that of PLL.
