In a previous paper we have described a contractile protein in the higher plants which is very similar to the actomyosin of muscle. The enzymatic activity of the crude extract of this protein was, however, rather low ...In a previous paper we have described a contractile protein in the higher plants which is very similar to the actomyosin of muscle. The enzymatic activity of the crude extract of this protein was, however, rather low compared with that of actomyosin and then further purification of the protein was carried out. In the present communication we would report the preliminary results obtained for the purification of the tobacco contractile protein by gel filtration and some of its ATPase properties. About 1000 g of the vascular bundles were isolated from freshly collected tobacco leaves and then frozen in a refrigerator at —20℃ overnight. One thousand ml of Weber-Edsall solution (composed of 0.6 M KCl, 0.04 M NaHCO3, 0.01 M Na2CO3,展开更多
文摘In a previous paper we have described a contractile protein in the higher plants which is very similar to the actomyosin of muscle. The enzymatic activity of the crude extract of this protein was, however, rather low compared with that of actomyosin and then further purification of the protein was carried out. In the present communication we would report the preliminary results obtained for the purification of the tobacco contractile protein by gel filtration and some of its ATPase properties. About 1000 g of the vascular bundles were isolated from freshly collected tobacco leaves and then frozen in a refrigerator at —20℃ overnight. One thousand ml of Weber-Edsall solution (composed of 0.6 M KCl, 0.04 M NaHCO3, 0.01 M Na2CO3,
