Eleven microsatellite(SSR)markers were used to evaluate the genetic diversity and paternal parents of a seed orchard for Pinus massoniana planted at Wuyi Forest Farm,Fujian Province.The seed orchard contains 286 open_...Eleven microsatellite(SSR)markers were used to evaluate the genetic diversity and paternal parents of a seed orchard for Pinus massoniana planted at Wuyi Forest Farm,Fujian Province.The seed orchard contains 286 open_pollinated progenies derived from 129 candidate paternal clones and three maternal trees.Both the parental and progeny populations displayed high genetic diversity(h),with the estimated h values of 0.543 8 and 0.455 6,respectively.Paternal clones contributed differently to the progeny;only 5.3%～15.8% clones contributed 27.08%～48.96% pollen.More strikingly,one paternal clone contributed 10% and 17% pollen,respectively,to two maternal trees.It was found that the mating compatibility of a clone was not related to its origin.There was no significant influence of the physical location of a clone on the amount of paternal constituent and genetic diversity.The rate of contamination by pollen outside the seed orchard was as high as 9.44%.No selfing progeny was observed,suggesting that selfing depression will not be an issue in the seed orchard of P.massoniana.展开更多
According to the genetic difference detected by means of RAPD markers, the methods of distinguish among 2 Liriodendron species and their interspecific hybrid were discussed, and the relationship between genetic distan...According to the genetic difference detected by means of RAPD markers, the methods of distinguish among 2 Liriodendron species and their interspecific hybrid were discussed, and the relationship between genetic distances of parents and their hybrids performance were analysed. Of the total 136 bands amplified with 25 10 mer primers, 3 presented in all 5 L.chinense sample trees, but absented in all 5 L.tulipifera sample trees. On the contrary, 8 presented in all 5 L.tulipifera sample trees, but absented in all 5 L.chinense sample trees. The 11 fragments could be detected in all the 6 hybrids sample trees. It was considered that 11 fragments could be used as markers in the identification. The range of genetic distance among 5 L.chinense sample trees, among the sample trees between L.chinense and L.tulipifera and between L.chinense and the hybrid were not overlapped. Therefore, the genetic distance between an unidentified tree and a L.chinense tree could also be used in the hybrid identification. The 16 sample trees were grouped correctly. So, the UPGMA (unweighted pair group methods of arithmetic (average))cluster analysis method was useful in the (hybrid) species identification. The mean height and basae diameter increment of families after the first growing season showed a significant guadratic cure correlation with genetic distances of parents. Therefore, the genetic distance had high potential for the choice of parents and the heterosis prediction in cross breeding of Liriodendron.展开更多
The cone and seed characters of Pinus Massoniana were investigated in a clonal seed orchard for two successive years(1998 and 1999).The results showed that significant genetic variation existed among clones,while vari...The cone and seed characters of Pinus Massoniana were investigated in a clonal seed orchard for two successive years(1998 and 1999).The results showed that significant genetic variation existed among clones,while variation within clone had not come up to remarkable standard.Broad sense herit ability of cone and seed characters in P.massoniana were influenced by genetic factors,most of them were at over middle level.There was a extremely significant difference in the correlation coefficients among the characters of cone and seeds,and the correlation coefficients of the same character in different years were at the extremely significant level,which indicated that the clones were quite stable from year to year in cone and seed quality and size.At last,the comprehensive evaluation was estimated in 140 clones by main index of reproductive capacity.The genetic gain of cone yield of a single tree,the seed numble of a single cone,and full seed percentage,and reselection of clones were discussed under different selection intensities.展开更多
文摘Eleven microsatellite(SSR)markers were used to evaluate the genetic diversity and paternal parents of a seed orchard for Pinus massoniana planted at Wuyi Forest Farm,Fujian Province.The seed orchard contains 286 open_pollinated progenies derived from 129 candidate paternal clones and three maternal trees.Both the parental and progeny populations displayed high genetic diversity(h),with the estimated h values of 0.543 8 and 0.455 6,respectively.Paternal clones contributed differently to the progeny;only 5.3%～15.8% clones contributed 27.08%～48.96% pollen.More strikingly,one paternal clone contributed 10% and 17% pollen,respectively,to two maternal trees.It was found that the mating compatibility of a clone was not related to its origin.There was no significant influence of the physical location of a clone on the amount of paternal constituent and genetic diversity.The rate of contamination by pollen outside the seed orchard was as high as 9.44%.No selfing progeny was observed,suggesting that selfing depression will not be an issue in the seed orchard of P.massoniana.
文摘According to the genetic difference detected by means of RAPD markers, the methods of distinguish among 2 Liriodendron species and their interspecific hybrid were discussed, and the relationship between genetic distances of parents and their hybrids performance were analysed. Of the total 136 bands amplified with 25 10 mer primers, 3 presented in all 5 L.chinense sample trees, but absented in all 5 L.tulipifera sample trees. On the contrary, 8 presented in all 5 L.tulipifera sample trees, but absented in all 5 L.chinense sample trees. The 11 fragments could be detected in all the 6 hybrids sample trees. It was considered that 11 fragments could be used as markers in the identification. The range of genetic distance among 5 L.chinense sample trees, among the sample trees between L.chinense and L.tulipifera and between L.chinense and the hybrid were not overlapped. Therefore, the genetic distance between an unidentified tree and a L.chinense tree could also be used in the hybrid identification. The 16 sample trees were grouped correctly. So, the UPGMA (unweighted pair group methods of arithmetic (average))cluster analysis method was useful in the (hybrid) species identification. The mean height and basae diameter increment of families after the first growing season showed a significant guadratic cure correlation with genetic distances of parents. Therefore, the genetic distance had high potential for the choice of parents and the heterosis prediction in cross breeding of Liriodendron.
文摘The cone and seed characters of Pinus Massoniana were investigated in a clonal seed orchard for two successive years(1998 and 1999).The results showed that significant genetic variation existed among clones,while variation within clone had not come up to remarkable standard.Broad sense herit ability of cone and seed characters in P.massoniana were influenced by genetic factors,most of them were at over middle level.There was a extremely significant difference in the correlation coefficients among the characters of cone and seeds,and the correlation coefficients of the same character in different years were at the extremely significant level,which indicated that the clones were quite stable from year to year in cone and seed quality and size.At last,the comprehensive evaluation was estimated in 140 clones by main index of reproductive capacity.The genetic gain of cone yield of a single tree,the seed numble of a single cone,and full seed percentage,and reselection of clones were discussed under different selection intensities.
