胶质母细胞瘤(GBM)是人类中枢神经系统肿瘤中发病率高、侵袭性强的恶性肿瘤.胶质母细胞瘤发生和发展的原因尚不清楚.银杏叶(GF)提取物可以减少裸鼠U-87胶质瘤细胞系的生长,然而,作用机制不清楚.在本研究中,网络药理学、分子对接和细胞...胶质母细胞瘤(GBM)是人类中枢神经系统肿瘤中发病率高、侵袭性强的恶性肿瘤.胶质母细胞瘤发生和发展的原因尚不清楚.银杏叶(GF)提取物可以减少裸鼠U-87胶质瘤细胞系的生长,然而,作用机制不清楚.在本研究中,网络药理学、分子对接和细胞实验被用来阐释银杏叶治疗胶质母细胞瘤的潜在的分子作用机制.从TCMSP数据库中获得银杏叶的有效成分和作用靶点,STRING数据库用于蛋白质相互作用分析,Cytoscape软件构建“成分-靶点”网络和PPI网络.R语言分析用来GO富集分析和KEGG通路分析.另外,Auto Dock Vina软件进行活性成分与核心靶点的分子对接验证.最后,通过细胞实验验证GF中的有效化合物对胶质母细胞瘤的抑制作用.结果表明,银杏叶有27个潜在有效成分和112个潜在靶点.从6个数据库中筛选出7 435个胶质母细胞瘤作用靶点.银杏叶与胶质母细胞瘤交集靶点98个.银杏叶共有18种核心有效成分,如槲皮素、山柰酚、木犀草素等以及10个相对应核心靶点ESR1、RELA、FOS、CCD1、EGFR、AR、HIF1A、MAPK8、NCOA1和MDM2.富集分析结果表明,银杏叶治疗胶质母细胞瘤主要参与生物过程,如细胞对化学应激的反应、对氧化应激的响应和转录调节复合物等.KEGG信号通路主要富集在PI3K-Akt、MAPK和TNF信号通路等.分子对接的结果验证了银杏叶的有效成分槲皮素(QUE),木犀草素(QUE)和山奈酚(KAE)与10个相对应的核心靶点具有良好结合活性.体外细胞实验表明,槲皮素、木犀草素和山柰酚抑制了人胶质母细胞瘤U251细胞活力.可以得出结论,银杏叶通过多组分、多靶点和多通路治疗胶质母细胞瘤,为银杏叶治疗胶质母细胞瘤的临床应用提供了理论基础.展开更多
Circular dichroism(CD)has shown very interesting possibilities as a means to characterize the chiral signal of a chiral structure.Here,we theoretically demonstrated enhanced and tunable CD in the visible light regime ...Circular dichroism(CD)has shown very interesting possibilities as a means to characterize the chiral signal of a chiral structure.Here,we theoretically demonstrated enhanced and tunable CD in the visible light regime using a composite structure consisting of a double-layer metal grating gaped by a dielectric waveguide layer.Based on the coupling of the waveguide modes and the localized plasmonic resonances,the CD could reach a maximum value as high as 0.52 at635 nm,which is four times higher than the CD value obtained in a conventional double-layer grating without the waveguide coupling effect.Furthermore,the spectral positions of the enhanced CD bands could be easily tuned by controlling the structural parameters.The proposed hybrid double-grating and waveguide structures could have potential applications in chiral selective imaging,sensing and spectroscopy,especially where the transmission measurement is required.展开更多
基金Supported by the Doctoral Innovation Project of Linyi People’s Hospital(2018LYBS12)。
文摘胶质母细胞瘤(GBM)是人类中枢神经系统肿瘤中发病率高、侵袭性强的恶性肿瘤.胶质母细胞瘤发生和发展的原因尚不清楚.银杏叶(GF)提取物可以减少裸鼠U-87胶质瘤细胞系的生长,然而,作用机制不清楚.在本研究中,网络药理学、分子对接和细胞实验被用来阐释银杏叶治疗胶质母细胞瘤的潜在的分子作用机制.从TCMSP数据库中获得银杏叶的有效成分和作用靶点,STRING数据库用于蛋白质相互作用分析,Cytoscape软件构建“成分-靶点”网络和PPI网络.R语言分析用来GO富集分析和KEGG通路分析.另外,Auto Dock Vina软件进行活性成分与核心靶点的分子对接验证.最后,通过细胞实验验证GF中的有效化合物对胶质母细胞瘤的抑制作用.结果表明,银杏叶有27个潜在有效成分和112个潜在靶点.从6个数据库中筛选出7 435个胶质母细胞瘤作用靶点.银杏叶与胶质母细胞瘤交集靶点98个.银杏叶共有18种核心有效成分,如槲皮素、山柰酚、木犀草素等以及10个相对应核心靶点ESR1、RELA、FOS、CCD1、EGFR、AR、HIF1A、MAPK8、NCOA1和MDM2.富集分析结果表明,银杏叶治疗胶质母细胞瘤主要参与生物过程,如细胞对化学应激的反应、对氧化应激的响应和转录调节复合物等.KEGG信号通路主要富集在PI3K-Akt、MAPK和TNF信号通路等.分子对接的结果验证了银杏叶的有效成分槲皮素(QUE),木犀草素(QUE)和山奈酚(KAE)与10个相对应的核心靶点具有良好结合活性.体外细胞实验表明,槲皮素、木犀草素和山柰酚抑制了人胶质母细胞瘤U251细胞活力.可以得出结论,银杏叶通过多组分、多靶点和多通路治疗胶质母细胞瘤,为银杏叶治疗胶质母细胞瘤的临床应用提供了理论基础.
基金Project supported by the National Natural Science Foundation of China(Grant No.11474215)。
文摘Circular dichroism(CD)has shown very interesting possibilities as a means to characterize the chiral signal of a chiral structure.Here,we theoretically demonstrated enhanced and tunable CD in the visible light regime using a composite structure consisting of a double-layer metal grating gaped by a dielectric waveguide layer.Based on the coupling of the waveguide modes and the localized plasmonic resonances,the CD could reach a maximum value as high as 0.52 at635 nm,which is four times higher than the CD value obtained in a conventional double-layer grating without the waveguide coupling effect.Furthermore,the spectral positions of the enhanced CD bands could be easily tuned by controlling the structural parameters.The proposed hybrid double-grating and waveguide structures could have potential applications in chiral selective imaging,sensing and spectroscopy,especially where the transmission measurement is required.
