目的探讨人参炔三醇(panaxytriol,PXT)抑制氧化低密度脂蛋白(oxidized low density lipoprotein,oxLDL)诱导单核巨噬细胞脂质沉积的作用机制。方法单核细胞THP-1经佛波酯(phorbol-12-myristate-13-acetate,PMA)诱导成巨噬细胞后,采用不...目的探讨人参炔三醇(panaxytriol,PXT)抑制氧化低密度脂蛋白(oxidized low density lipoprotein,oxLDL)诱导单核巨噬细胞脂质沉积的作用机制。方法单核细胞THP-1经佛波酯(phorbol-12-myristate-13-acetate,PMA)诱导成巨噬细胞后,采用不同浓度(0、1、5、10、20μmol/L)PXT处理单核巨噬细胞24小时,通过MTT法检测其对细胞存活率的影响。THP-1单核巨噬细胞经PMA刺激后,利用oxLDL诱导脂质蓄积,PXT处理后利用流式细胞仪,荧光显微镜和油红O染色检测巨噬细胞对oxLDL的摄取。采用Western blot检测细胞内白细胞分化抗原36(cluster of differentiation,CD36)、三磷酸腺苷结合盒转运体A1(ATP-binding cassette transporter A1,ABCA1)、清道夫受体B1(scavenger receptor class B type 1,SRB1)的蛋白表达水平变化。结果MTT实验结果显示,与正常组比较,20μmol/L PXT处理THP-1单核巨噬细胞24小时后显著抑制了细胞活力(P<0.05),且其他浓度对细胞活力均无显著影响。流式细胞检测、细胞荧光成像和油红O染色结果显示,与模型组比较,PXT浓度依赖地降低巨噬细胞内DiI-oxLDL荧光强度与细胞内脂质蓄积(P<0.05)。Western blot结果显示,与模型组比较,PXT浓度依赖地降低了巨噬细胞中CD36蛋白的表达(P<0.05),而对其他蛋白无显著影响。结论PXT通过抑制清道夫受体CD36的表达,抑制巨噬细胞对oxLDL的吞噬及泡沫细胞的形成,具有潜在的抗动脉粥样硬化活性。展开更多
Trapped atoms on photonic structures inspire many novel quantum devices for quantum information processing and quantum sensing.Here,we demonstrate a hybrid photonic-atom chip platform based on a Ga N-onsapphire chip a...Trapped atoms on photonic structures inspire many novel quantum devices for quantum information processing and quantum sensing.Here,we demonstrate a hybrid photonic-atom chip platform based on a Ga N-onsapphire chip and the transport of an ensemble of atoms from free space towards the chip with an optical conveyor belts.Due to our platform’s complete optical accessibility and careful control of atomic motion near the chip with a conveyor belt,successful atomic transport towards the chip is made possible.The maximum transport efficiency of atoms is about 50%with a transport distance of 500μm.Our results open up a new route toward the efficient loading of cold atoms into the evanescent-field trap formed by the photonic integrated circuits,which promises strong and controllable interactions between single atoms and single photons.展开更多
文摘目的探讨人参炔三醇(panaxytriol,PXT)抑制氧化低密度脂蛋白(oxidized low density lipoprotein,oxLDL)诱导单核巨噬细胞脂质沉积的作用机制。方法单核细胞THP-1经佛波酯(phorbol-12-myristate-13-acetate,PMA)诱导成巨噬细胞后,采用不同浓度(0、1、5、10、20μmol/L)PXT处理单核巨噬细胞24小时,通过MTT法检测其对细胞存活率的影响。THP-1单核巨噬细胞经PMA刺激后,利用oxLDL诱导脂质蓄积,PXT处理后利用流式细胞仪,荧光显微镜和油红O染色检测巨噬细胞对oxLDL的摄取。采用Western blot检测细胞内白细胞分化抗原36(cluster of differentiation,CD36)、三磷酸腺苷结合盒转运体A1(ATP-binding cassette transporter A1,ABCA1)、清道夫受体B1(scavenger receptor class B type 1,SRB1)的蛋白表达水平变化。结果MTT实验结果显示,与正常组比较,20μmol/L PXT处理THP-1单核巨噬细胞24小时后显著抑制了细胞活力(P<0.05),且其他浓度对细胞活力均无显著影响。流式细胞检测、细胞荧光成像和油红O染色结果显示,与模型组比较,PXT浓度依赖地降低巨噬细胞内DiI-oxLDL荧光强度与细胞内脂质蓄积(P<0.05)。Western blot结果显示,与模型组比较,PXT浓度依赖地降低了巨噬细胞中CD36蛋白的表达(P<0.05),而对其他蛋白无显著影响。结论PXT通过抑制清道夫受体CD36的表达,抑制巨噬细胞对oxLDL的吞噬及泡沫细胞的形成,具有潜在的抗动脉粥样硬化活性。
基金supported by the National Key R&D Program(Grant No.2021YFF0603701)the National Natural Science Foundation of China(Grant Nos.U21A20433,U21A6006,92265210,12104441,12134014,61905234,and 11974335)+1 种基金the USTC Research Funds of the Double First-Class Initiative(Grant No.YD2030002007),USTC Research Funds of the Double First-Class Initiativesupported by the Fundamental Research Funds for the Central Universities。
文摘Trapped atoms on photonic structures inspire many novel quantum devices for quantum information processing and quantum sensing.Here,we demonstrate a hybrid photonic-atom chip platform based on a Ga N-onsapphire chip and the transport of an ensemble of atoms from free space towards the chip with an optical conveyor belts.Due to our platform’s complete optical accessibility and careful control of atomic motion near the chip with a conveyor belt,successful atomic transport towards the chip is made possible.The maximum transport efficiency of atoms is about 50%with a transport distance of 500μm.Our results open up a new route toward the efficient loading of cold atoms into the evanescent-field trap formed by the photonic integrated circuits,which promises strong and controllable interactions between single atoms and single photons.
