An Arabidopsis cell death mutation locus was mapped to chromosome 2 between lGS1 and mi421. The YAC clone ends, CIC9A3R, CIC11C7L, CIC2G5R and RFLP marker CDs3 within this interval, were used to probe TAMU BAC library...An Arabidopsis cell death mutation locus was mapped to chromosome 2 between lGS1 and mi421. The YAC clone ends, CIC9A3R, CIC11C7L, CIC2G5R and RFLP marker CDs3 within this interval, were used to probe TAMU BAC library and 31 BAC clones were obtained. A BAC contig encompassing the mutation locus, which consists of T6P5, T7M23, T12A21, T8L6 and T18A18, was identified by Southern hybridization with the BAC ends as probes. 11 CAPS and 12 STS markers were developed in this region. These results will facilitate map-based cloning of the genes and sequencing of the genomic DNA in this region.展开更多
[Objective] The paper was to study the dynamic responses of super rice Ilyou602 plants to different nitrogen levels and configuration modes. [Method] With super rice Ilyou602 as test material, the dynamic changes of t...[Objective] The paper was to study the dynamic responses of super rice Ilyou602 plants to different nitrogen levels and configuration modes. [Method] With super rice Ilyou602 as test material, the dynamic changes of total N content in functional leaves, leaf sheaths and stems under different, application amount of nitrogen and configuration modes were analyzed. [Result] The highest total N content of functional leaves under different N levels appeared in the different periods, which was the highest during rehydration period in A3 level. The total N content in various plant positions during full heading stage and maturity stage showed fertilization treatments 〉 CK, functional leaves 〉 leaf sheaths 〉 stems. The total N content in different plant positions showed obvious difference in different treatments. When the super rice Ilyou602 was planted in Chengdu plain, the proper application level of N and configuration mode was as follows： 165 kg/hm2, fertilizer： topdressing = 6：4. [Conclusion] The study provided theoretical basis for nitrogen regulation of high yield cultivation of super hybrid rice.展开更多
文摘An Arabidopsis cell death mutation locus was mapped to chromosome 2 between lGS1 and mi421. The YAC clone ends, CIC9A3R, CIC11C7L, CIC2G5R and RFLP marker CDs3 within this interval, were used to probe TAMU BAC library and 31 BAC clones were obtained. A BAC contig encompassing the mutation locus, which consists of T6P5, T7M23, T12A21, T8L6 and T18A18, was identified by Southern hybridization with the BAC ends as probes. 11 CAPS and 12 STS markers were developed in this region. These results will facilitate map-based cloning of the genes and sequencing of the genomic DNA in this region.
基金Supported by Major Project of "Eleventh Five-Year" National Science and Technology Support Plan (2004BA520A05)Major Special Research Project of Ministry of Agriculture(Development and Integration of Super Rice Cultivation Technology)+1 种基金Science and Technology Support Program of Sichuan Province (2008FZ0036)Key Scientific and Technological Project of Deyang City (2007ND028)~~
文摘[Objective] The paper was to study the dynamic responses of super rice Ilyou602 plants to different nitrogen levels and configuration modes. [Method] With super rice Ilyou602 as test material, the dynamic changes of total N content in functional leaves, leaf sheaths and stems under different, application amount of nitrogen and configuration modes were analyzed. [Result] The highest total N content of functional leaves under different N levels appeared in the different periods, which was the highest during rehydration period in A3 level. The total N content in various plant positions during full heading stage and maturity stage showed fertilization treatments 〉 CK, functional leaves 〉 leaf sheaths 〉 stems. The total N content in different plant positions showed obvious difference in different treatments. When the super rice Ilyou602 was planted in Chengdu plain, the proper application level of N and configuration mode was as follows： 165 kg/hm2, fertilizer： topdressing = 6：4. [Conclusion] The study provided theoretical basis for nitrogen regulation of high yield cultivation of super hybrid rice.
