The cloning in E. coli of a cholerae toxin gene that is A^-B^+ has been successfully constructed by using DNA recombinant techniques. E. coli cells carrying the recombinant plasmid pMM-CTB have been shown to produce a...The cloning in E. coli of a cholerae toxin gene that is A^-B^+ has been successfully constructed by using DNA recombinant techniques. E. coli cells carrying the recombinant plasmid pMM-CTB have been shown to produce a large amount of CTB subunits which are secreted as extracellular proteins.展开更多
An engineered E. coli strain containing high expression level of CT-B subunits has beenobtained by the application of recombinant DNA techniques. The B subunit can be secretedinto the medium and reaches 20- 40 μg/ml ...An engineered E. coli strain containing high expression level of CT-B subunits has beenobtained by the application of recombinant DNA techniques. The B subunit can be secretedinto the medium and reaches 20- 40 μg/ml when this strain is incubated in a 50 1 fermenta-tion tank. The CT-B subunit purified with affinity chromatography in E. coli has the samecharacters as the natural CT- B subunit in molecular weight, N terminal amino acid analysisand antigenicity. The CT-B subunit has good immunogenicity and can be used as a preparation for protect-ing against diarrhea caused by V. cholera and enterotoxigenic E. coli. It can also be usedas a vector for hepatins.展开更多
基金Project supported by the National Natural Science Foundation of China
文摘The cloning in E. coli of a cholerae toxin gene that is A^-B^+ has been successfully constructed by using DNA recombinant techniques. E. coli cells carrying the recombinant plasmid pMM-CTB have been shown to produce a large amount of CTB subunits which are secreted as extracellular proteins.
文摘An engineered E. coli strain containing high expression level of CT-B subunits has beenobtained by the application of recombinant DNA techniques. The B subunit can be secretedinto the medium and reaches 20- 40 μg/ml when this strain is incubated in a 50 1 fermenta-tion tank. The CT-B subunit purified with affinity chromatography in E. coli has the samecharacters as the natural CT- B subunit in molecular weight, N terminal amino acid analysisand antigenicity. The CT-B subunit has good immunogenicity and can be used as a preparation for protect-ing against diarrhea caused by V. cholera and enterotoxigenic E. coli. It can also be usedas a vector for hepatins.
