[Objective] This study was conducted to clarify the biological information of PHYB genes in upland cotton （Gossypium hirsutum）. [Method] Two PHYB genes were identified from the genome database of allotetraploid cott...[Objective] This study was conducted to clarify the biological information of PHYB genes in upland cotton （Gossypium hirsutum）. [Method] Two PHYB genes were identified from the genome database of allotetraploid cotton （G. hirsutum L. acc. TM-1）, and were found to be distributed on subgenomes A10 and D10. And then bioinformatic analysis on these two genes were performed. [Result] The PHYB genes of upland cotton had the same motifs and domains with the PHYB genes in other plant species, and even the number and location of the motifs and domains of these PHYB genes were consistent. The PHYB amino acid sequence alignment and the phylogenetic tree constructed based on PHYB amino acid sequence of these plant species indicated that the two PHYB genes in upland cotton had higher homology and closer evolutionary relationships with cocoa （Theobroma cacao）, but lower similarity to PHYB genes in monocotyledonous plants, such as rice （Oryza saitva） and corn （Zea mays）. The comparison of PHYB gene structure also revealed that plant PHYB gene was more conserved during evolution. The autophosphorylation of dozens of phosphorylation sites in upland cotton PHYB gene may be essential for the functions of phytochromes and plays a significant role in regulating phytochrome-mediated signal transduction pathways. [Conclusion] The results of this paper will provide a theoretical basis for the cloning and functional research of PHYB genes.展开更多
目的了解贵阳市适龄女性人群对现有HPV疫苗的知识、态度和行为(Knowledge,attitude and practice,KAP)现况,运用结构方程模型分析三者之间的关系,为HPV疫苗健康教育提供科学依据。方法采用多阶段分层随机整群抽样方法随机抽取了云岩、...目的了解贵阳市适龄女性人群对现有HPV疫苗的知识、态度和行为(Knowledge,attitude and practice,KAP)现况,运用结构方程模型分析三者之间的关系,为HPV疫苗健康教育提供科学依据。方法采用多阶段分层随机整群抽样方法随机抽取了云岩、花溪和观山湖区1680名适龄女性,运用自行设计的问卷进行调查,用SPSS 19.0进行统计描述,AMOS 23.0进行结构方程模型的建立及初步探讨贵阳市适龄女性知识-态度-行为的作用路径及可能的影响因素。结果HPV疫苗总知晓率为34.6%,积极态度率为90.7%,积极行为率为41.7%。不同问题的知晓率在3.3%~93.0%之间。结构方程模型拟合情况良好。各潜变量之间的关系均为正向相关,且各标准化路径均具有统计学意义(P<0.001),知识对态度的影响路径系数为0.71;态度对行为的影响路径系数为0.22;知识对预防行为的直接效果高于间接效果,态度在知识对行为的作用过程中呈现部分中介作用。结论贵阳市适龄女性对HPV疫苗的知晓率和积极行为还有待提高,本研究结构方程模型提示当适龄女性对HPV疫苗相关知识了解充分可以很容易产生积极态度和行为,且行为不仅会受到态度的直接影响,可能还受其他因素的影响。展开更多
基金Supported by National Natural Science Foundation of China(31301682)the Agricultural Science Independent Innovation Foundation of Jiangsu Province[CX(14)5009]+1 种基金National Key Special Project for Breeding and Cultivation of GMO Varieties of China(2013ZX08005)the Jiangsu Province Science and Technology Support Program(BE2014389)~~
文摘[Objective] This study was conducted to clarify the biological information of PHYB genes in upland cotton （Gossypium hirsutum）. [Method] Two PHYB genes were identified from the genome database of allotetraploid cotton （G. hirsutum L. acc. TM-1）, and were found to be distributed on subgenomes A10 and D10. And then bioinformatic analysis on these two genes were performed. [Result] The PHYB genes of upland cotton had the same motifs and domains with the PHYB genes in other plant species, and even the number and location of the motifs and domains of these PHYB genes were consistent. The PHYB amino acid sequence alignment and the phylogenetic tree constructed based on PHYB amino acid sequence of these plant species indicated that the two PHYB genes in upland cotton had higher homology and closer evolutionary relationships with cocoa （Theobroma cacao）, but lower similarity to PHYB genes in monocotyledonous plants, such as rice （Oryza saitva） and corn （Zea mays）. The comparison of PHYB gene structure also revealed that plant PHYB gene was more conserved during evolution. The autophosphorylation of dozens of phosphorylation sites in upland cotton PHYB gene may be essential for the functions of phytochromes and plays a significant role in regulating phytochrome-mediated signal transduction pathways. [Conclusion] The results of this paper will provide a theoretical basis for the cloning and functional research of PHYB genes.
文摘目的了解贵阳市适龄女性人群对现有HPV疫苗的知识、态度和行为(Knowledge,attitude and practice,KAP)现况,运用结构方程模型分析三者之间的关系,为HPV疫苗健康教育提供科学依据。方法采用多阶段分层随机整群抽样方法随机抽取了云岩、花溪和观山湖区1680名适龄女性,运用自行设计的问卷进行调查,用SPSS 19.0进行统计描述,AMOS 23.0进行结构方程模型的建立及初步探讨贵阳市适龄女性知识-态度-行为的作用路径及可能的影响因素。结果HPV疫苗总知晓率为34.6%,积极态度率为90.7%,积极行为率为41.7%。不同问题的知晓率在3.3%~93.0%之间。结构方程模型拟合情况良好。各潜变量之间的关系均为正向相关,且各标准化路径均具有统计学意义(P<0.001),知识对态度的影响路径系数为0.71;态度对行为的影响路径系数为0.22;知识对预防行为的直接效果高于间接效果,态度在知识对行为的作用过程中呈现部分中介作用。结论贵阳市适龄女性对HPV疫苗的知晓率和积极行为还有待提高,本研究结构方程模型提示当适龄女性对HPV疫苗相关知识了解充分可以很容易产生积极态度和行为,且行为不仅会受到态度的直接影响,可能还受其他因素的影响。
