Abstract Inter-simple sequence repeat （ISSR） markers were used to determine the genetic variation and genetic differentiation of cultured and wild populations of Trachidermus fasciatus, an endangered catadromous fis...Abstract Inter-simple sequence repeat （ISSR） markers were used to determine the genetic variation and genetic differentiation of cultured and wild populations of Trachidermus fasciatus, an endangered catadromous fish species in China. Six selected primers were used to amplify DNA samples from 85 individuals, and 353 loci were detected. Relatively low genetic diversity was detected in the cultured population （the percentage of polymorphic loci PPL=73.80%, Nei＇s gene diversity h--0.178 2, Shannon information index I=0.276 9）. However, the genetic diversity at the species level was relatively high （PPL-91.78%; h = 0.258 3, I= 0.398 6）. The UPGMA tree grouped together the genotypes almost according to their cultured and wild origin, showing distinct differences in genetic structure between wild and cultured populations. The pairwise F^t values confirmed significant genetic differentiation between wild and cultured samples. The cultivated population seems to be low in genetic diversity as a result of detrimental genetic effects in the captive population. The results suggest that ISSR markers are effective for rapid assessment of the degree of diversity of a population, thus giving important topical information relevant to preserving endangered species.展开更多
基金Supported by the Key Laboratory of Freshwater Biodiversity Conservation and Utilization, Ministry of Agriculture (No. LFBCU0713)the Special Research Foundation for Public Welfare Marine Program (No. 200905019-2)
文摘Abstract Inter-simple sequence repeat （ISSR） markers were used to determine the genetic variation and genetic differentiation of cultured and wild populations of Trachidermus fasciatus, an endangered catadromous fish species in China. Six selected primers were used to amplify DNA samples from 85 individuals, and 353 loci were detected. Relatively low genetic diversity was detected in the cultured population （the percentage of polymorphic loci PPL=73.80%, Nei＇s gene diversity h--0.178 2, Shannon information index I=0.276 9）. However, the genetic diversity at the species level was relatively high （PPL-91.78%; h = 0.258 3, I= 0.398 6）. The UPGMA tree grouped together the genotypes almost according to their cultured and wild origin, showing distinct differences in genetic structure between wild and cultured populations. The pairwise F^t values confirmed significant genetic differentiation between wild and cultured samples. The cultivated population seems to be low in genetic diversity as a result of detrimental genetic effects in the captive population. The results suggest that ISSR markers are effective for rapid assessment of the degree of diversity of a population, thus giving important topical information relevant to preserving endangered species.
