Being a promising anticancer agent, taxol draws more and more attention. This paper has studied the UV spectrum of taxol and fluorescence spectrum of BSA taxol conjugate. A method of testing the modification radio is ...Being a promising anticancer agent, taxol draws more and more attention. This paper has studied the UV spectrum of taxol and fluorescence spectrum of BSA taxol conjugate. A method of testing the modification radio is presented and the structure of the conjugate was discussed.展开更多
<正> Detailed studies were carried out on equilibrium dialysis of the binding of Ni2++ ion to human scrum albumin (HSA) and bovine serum albumin (BSA).The successive stability constants were obtained by the Icfi...<正> Detailed studies were carried out on equilibrium dialysis of the binding of Ni2++ ion to human scrum albumin (HSA) and bovine serum albumin (BSA).The successive stability constants were obtained by the Icfisi squares fitting.The eight binding sites found for both Ni(Ⅱ)-HSA and Ni(Ⅱ)-BSA systems can be divided into two different sets; and for both systems,there exist two identical prior binding sites where the bound Ni2+ ions can he con sidered as allosteric effectors,which induce the allosteric effect in accordance with the model proposed by Moeod et al As indicated by allosteric parameters,the ability of R-state to bind Ni2+ ions is ca 100 times as much as that of T state,and the conformation of HSA is markedly tenser than that of BSA.展开更多
The effects of Ce4+ on cell survival, expression and activity of matrix metalloproteinase-9 (MMP-9) with WIT colorimetry and gelatin zymography assays in human bladder carcinoma cell line was studied. The results indi...The effects of Ce4+ on cell survival, expression and activity of matrix metalloproteinase-9 (MMP-9) with WIT colorimetry and gelatin zymography assays in human bladder carcinoma cell line was studied. The results indicate that the lower concentration of Ce4+(0.01 mmol (.) L-1) has no influence on cell growth, but inhibits extremely expression of MMP-9 and increases its activity, whereas the higher concentration of Ce4+(1.0 mmol (.) L-1) can inhibit all of them. The results raise the possibility that Ce4+ may have beneficial effects in attenuating invasion and metastasis of malignant tumors.展开更多
The heptadecapeptide orphanin FQ (OFQ) is a recently discovered neuropeptide that exhibits structural features reminiscent of the opioid peptides and that is an endogenous ligant to a G protein-coupled receptor sequen...The heptadecapeptide orphanin FQ (OFQ) is a recently discovered neuropeptide that exhibits structural features reminiscent of the opioid peptides and that is an endogenous ligant to a G protein-coupled receptor sequentially related to the opioid receptors. OFQ was originally isolated from brain, but the presence of OFQ in peripheral tissues, especially in cardiovascular system, has not been clarified. The present study was designed to investigate the peripheral tissue distribution of OFQ precusor mRNA in stroke-prone spontaneously hypertensive rats (SHRSP) and compare the difference of OFQ precusor mRNA expression in aorta or cultured vascular smooth muscle cells (VSMCs) between SHRSP and wistar-Kyoto normotensive (WKY) rats. By using quantitative reverse transcription-polymerase chain reaction (RT-PCR), OFQ precusor mRNA was detected in aorta and ovary at high levels comparable with the amounts found in brain. Moderate expression was found in testis, while a little OFQ precusor mRNA could be detected in atrium. All other peripheral tissues examined from SHRSP, including ventricle, liver, lung and kidney, showed no expression of OFQ precusor mRNA. In the vascular system, OFQ precusor mRNA was expressed in aorta, pulmonary artery, renal artery and vein at high levels comparable with the amounts found in brain. We also found that OFQ precusor mRNA levels were much higher in aorta or cultured VSMCs from SHRSP than those from WKY rats. In conclusion, the present study has shown that OFQ precusor mRNA is present in some peripheral tissues, especially in cardiovascular and reproductive system, suggesting that OFQ possibly involves in the regulation of cardiovascular and reproductive functions.展开更多
文摘Being a promising anticancer agent, taxol draws more and more attention. This paper has studied the UV spectrum of taxol and fluorescence spectrum of BSA taxol conjugate. A method of testing the modification radio is presented and the structure of the conjugate was discussed.
基金Project supported by the National Natural Science Foundation of China
文摘<正> Detailed studies were carried out on equilibrium dialysis of the binding of Ni2++ ion to human scrum albumin (HSA) and bovine serum albumin (BSA).The successive stability constants were obtained by the Icfisi squares fitting.The eight binding sites found for both Ni(Ⅱ)-HSA and Ni(Ⅱ)-BSA systems can be divided into two different sets; and for both systems,there exist two identical prior binding sites where the bound Ni2+ ions can he con sidered as allosteric effectors,which induce the allosteric effect in accordance with the model proposed by Moeod et al As indicated by allosteric parameters,the ability of R-state to bind Ni2+ ions is ca 100 times as much as that of T state,and the conformation of HSA is markedly tenser than that of BSA.
文摘The effects of Ce4+ on cell survival, expression and activity of matrix metalloproteinase-9 (MMP-9) with WIT colorimetry and gelatin zymography assays in human bladder carcinoma cell line was studied. The results indicate that the lower concentration of Ce4+(0.01 mmol (.) L-1) has no influence on cell growth, but inhibits extremely expression of MMP-9 and increases its activity, whereas the higher concentration of Ce4+(1.0 mmol (.) L-1) can inhibit all of them. The results raise the possibility that Ce4+ may have beneficial effects in attenuating invasion and metastasis of malignant tumors.
文摘The heptadecapeptide orphanin FQ (OFQ) is a recently discovered neuropeptide that exhibits structural features reminiscent of the opioid peptides and that is an endogenous ligant to a G protein-coupled receptor sequentially related to the opioid receptors. OFQ was originally isolated from brain, but the presence of OFQ in peripheral tissues, especially in cardiovascular system, has not been clarified. The present study was designed to investigate the peripheral tissue distribution of OFQ precusor mRNA in stroke-prone spontaneously hypertensive rats (SHRSP) and compare the difference of OFQ precusor mRNA expression in aorta or cultured vascular smooth muscle cells (VSMCs) between SHRSP and wistar-Kyoto normotensive (WKY) rats. By using quantitative reverse transcription-polymerase chain reaction (RT-PCR), OFQ precusor mRNA was detected in aorta and ovary at high levels comparable with the amounts found in brain. Moderate expression was found in testis, while a little OFQ precusor mRNA could be detected in atrium. All other peripheral tissues examined from SHRSP, including ventricle, liver, lung and kidney, showed no expression of OFQ precusor mRNA. In the vascular system, OFQ precusor mRNA was expressed in aorta, pulmonary artery, renal artery and vein at high levels comparable with the amounts found in brain. We also found that OFQ precusor mRNA levels were much higher in aorta or cultured VSMCs from SHRSP than those from WKY rats. In conclusion, the present study has shown that OFQ precusor mRNA is present in some peripheral tissues, especially in cardiovascular and reproductive system, suggesting that OFQ possibly involves in the regulation of cardiovascular and reproductive functions.
