妊娠肝内胆汁淤积症(Intrahepatic Chol-estasis of Pregnancy简称ICP),是一种在妊娠期出现搔痒及黄疸为特征的合并症,以往认为系良性经过,对母婴无不良影响,无需特殊监护。近年来文献报告增多,国外发病率为0.68～3.25%,国内为0.13～3....妊娠肝内胆汁淤积症(Intrahepatic Chol-estasis of Pregnancy简称ICP),是一种在妊娠期出现搔痒及黄疸为特征的合并症,以往认为系良性经过,对母婴无不良影响,无需特殊监护。近年来文献报告增多,国外发病率为0.68～3.25%,国内为0.13～3.4%,并可引起严重母婴并发症,故ICP 应列入高危妊娠范畴。现将我院观察到的71例报告如下。展开更多
Objective The detection of RNA single nucleotide polymorphism(SNP)is of great importance due to their association with protein expression related to various diseases and drug responses.At present,splintR ligase-assist...Objective The detection of RNA single nucleotide polymorphism(SNP)is of great importance due to their association with protein expression related to various diseases and drug responses.At present,splintR ligase-assisted methods are important approaches for RNA direct detection,but its specificity will be limited when the fidelity of ligases is not ideal.The aim of this study was to create a method to improve the specificity of splintR ligase for RNA detection.Methods In this study,a dualcompetitive-padlock-probe(DCPLP)assay without the need for additional enzymes or reactions is proposed to improve specificity of splintR ligase ligation.To verify the method,we employed dual competitive padlock probe-mediated rolling circle amplification(DCPLP-RCA)to genotype the CYP2C9 gene.Results The specificity was well improved through the competition and strand displacement of dual padlock probe,with an 83.26%reduction in nonspecific signal.By detecting synthetic RNA samples,the method demonstrated a dynamic detection range of 10 pmol/L-1 nmol/L.Furthermore,clinical samples were applied to the method to evaluate its performance,and the genotyping results were consistent with those obtained using the qPCR method.Conclusion This study has successfully established a highly specific direct RNA SNP detection method,and provided a novel avenue for accurate identification of various types of RNAs.展开更多
Objective To screen the target genes that are associated with survival of breast cancer(BRCA) and explore their prognostic values and immune correlations with BRCA using multiple databases..Methods The microarray expr...Objective To screen the target genes that are associated with survival of breast cancer(BRCA) and explore their prognostic values and immune correlations with BRCA using multiple databases..Methods The microarray expression datasets of BRCA were downloaded from the Gene Expresssion Omnibus database(GEO) and analyzed to obtain differentially expressed genes(DEGs). Hub genes were obtained by constructing and visualizing the protein-protein interaction network of DEGs. The key gene was determined using R language, STRING, and Cytoscape, and the differential expression of the key gene was verified using external datasets The Cancer Genome Atlas(TCGA) and quantitative real-time PCR(q RT-PCR) for BRCA tissues of 37 patients. The prognostic value and immunological correlation of UBE2C in BRCA were explored using R language, TIMER, and Gene Set Enrichment Analysis(GSEA).Results Of 10 hub genes seleceed from 302 DEGS, UBE2C was identified as the gene associated with BRCA survival. The expression of UBE2C was differentially upregulated in BRCA, as verified by TCGA and q RT-PCR. Prognostic analysis revealed that UBE2C served as an independent prognostic factor. High expression of UBE2C was associated with decreased immune infiltration levels of B cells, CD4+ T cells, CD8+ T cells, macrophages, and myeloid dendritic cells in BRCA tissue. The expression of UBE2C in BRCA showed a significant correlation with immune checkpoints genes PDCD1, CD274, and CTLA4 expressions. There was a positive correlation between the expression of UBE2C and the tumor mutational burden and microsatellite instability. GSEA demonstrated that UBE2C expression significantly enriched 786 immune-related gene sets.Conclusions UBE2C expression in BRCA tissues is closely related to the BRCA immune microenvironment and showes predictive values on the survivals and prognosis of BRCA patients and the effecacy of immunotherapy. UBE2C may be an potential immune-related prognostic biomarker for BRCA.展开更多
Acute fatty liver of pregnancy(AFLP)is an idiopathic disease with acute and critical onset.Although the incidence of AFLP is relatively low,its mortality remains high.When AFLP is complicated with multi-organ dysfunct...Acute fatty liver of pregnancy(AFLP)is an idiopathic disease with acute and critical onset.Although the incidence of AFLP is relatively low,its mortality remains high.When AFLP is complicated with multi-organ dysfunction syndrome(MODS)and severe infection,especially hematogenous infection caused by multidrug-resistant pathogens,treatment becomes extremely difficult with an even lower survival rate.In the present work,we reported a 31-year-old primipara woman who developed AFLP complicated with MODS and severe infection at 37+5 weeks of gestation and received treatment in our hospital.The result of fast blood culture was positive for carbapenem-resistant Klebsiella pneumonia(CRKP).Based on this finding in combination with the results of drug sensitivity test,the quadruple regime using scheme imipenem cilastatin sodium,tigecycline,polymyxin and fosfomycin was implemented,and the patient was successfully cured.Successful treatment of pregnant women with AFLP complicated with MODS and/or hematogenous infection of CRKP can be achieved by using individualized combined antibiotic therapies on the basis of fast blood culture and combined drug sensitivity test.In this case,the patient was in a critical situation.In the treatment process,clinicians,clinical pharmacists and microbiologists should cooperate with each other and discuss the treatment plan together according to the pathophysiological characteristics of the patient,which was one of the key factors for successful treatment of the patient.展开更多
Wnt5a is a secreted Wnt ligand that plays a critical role in cellular pathways and inflammatory diseases.The WNT5A gene encodes two protein isoforms,Wnt5a-long and Wnt5a-short,which differ based on different promoter ...Wnt5a is a secreted Wnt ligand that plays a critical role in cellular pathways and inflammatory diseases.The WNT5A gene encodes two protein isoforms,Wnt5a-long and Wnt5a-short,which differ based on different promoter methylation and have distinct functions.However,the mechanisms of the promoter methylation are unclear.Depending on the extent of promoter methylation,Wnt5a exerts both anti-inflammatory and proinflammatory effects in inflammatory diseases,which may be involved in different Wnt5a isoforms.Therefore,the Wnt5a isoforms may be potential diagnostic markers for inflammatory diseases and the mechanisms of the WNT5A gene promoter methylation need to be further investigated.展开更多
Tachydromia Meigen is a somewhat large genus in the family Empididae with 112 known species distributed worldwide.Ten species were previously known to occur in China.Here the genus Tachydromia is newly recorded from S...Tachydromia Meigen is a somewhat large genus in the family Empididae with 112 known species distributed worldwide.Ten species were previously known to occur in China.Here the genus Tachydromia is newly recorded from Shaanxi.The following two new species belonging to the Tachydromia arrogans species group are described:Tachydromia basiflava sp.nov.and Tachydromia crassisetosa sp.nov.A key to the species of Tachydromia from China is provided.展开更多
Genus Ptilomyia Coquillett(Diptera: Ephydridae) is reported from the Oriental Region and China for the first time. A new species, P. chinensis sp. nov., is described and illustrated. A key to the Oriental and Palaearc...Genus Ptilomyia Coquillett(Diptera: Ephydridae) is reported from the Oriental Region and China for the first time. A new species, P. chinensis sp. nov., is described and illustrated. A key to the Oriental and Palaearctic regions of Ptilomyia is given. The types are kept in the Entomological Museum of China Agricultural University(CAU), Beijing.展开更多
文摘妊娠肝内胆汁淤积症(Intrahepatic Chol-estasis of Pregnancy简称ICP),是一种在妊娠期出现搔痒及黄疸为特征的合并症,以往认为系良性经过,对母婴无不良影响,无需特殊监护。近年来文献报告增多,国外发病率为0.68～3.25%,国内为0.13～3.4%,并可引起严重母婴并发症,故ICP 应列入高危妊娠范畴。现将我院观察到的71例报告如下。
文摘Objective The detection of RNA single nucleotide polymorphism(SNP)is of great importance due to their association with protein expression related to various diseases and drug responses.At present,splintR ligase-assisted methods are important approaches for RNA direct detection,but its specificity will be limited when the fidelity of ligases is not ideal.The aim of this study was to create a method to improve the specificity of splintR ligase for RNA detection.Methods In this study,a dualcompetitive-padlock-probe(DCPLP)assay without the need for additional enzymes or reactions is proposed to improve specificity of splintR ligase ligation.To verify the method,we employed dual competitive padlock probe-mediated rolling circle amplification(DCPLP-RCA)to genotype the CYP2C9 gene.Results The specificity was well improved through the competition and strand displacement of dual padlock probe,with an 83.26%reduction in nonspecific signal.By detecting synthetic RNA samples,the method demonstrated a dynamic detection range of 10 pmol/L-1 nmol/L.Furthermore,clinical samples were applied to the method to evaluate its performance,and the genotyping results were consistent with those obtained using the qPCR method.Conclusion This study has successfully established a highly specific direct RNA SNP detection method,and provided a novel avenue for accurate identification of various types of RNAs.
文摘Objective To screen the target genes that are associated with survival of breast cancer(BRCA) and explore their prognostic values and immune correlations with BRCA using multiple databases..Methods The microarray expression datasets of BRCA were downloaded from the Gene Expresssion Omnibus database(GEO) and analyzed to obtain differentially expressed genes(DEGs). Hub genes were obtained by constructing and visualizing the protein-protein interaction network of DEGs. The key gene was determined using R language, STRING, and Cytoscape, and the differential expression of the key gene was verified using external datasets The Cancer Genome Atlas(TCGA) and quantitative real-time PCR(q RT-PCR) for BRCA tissues of 37 patients. The prognostic value and immunological correlation of UBE2C in BRCA were explored using R language, TIMER, and Gene Set Enrichment Analysis(GSEA).Results Of 10 hub genes seleceed from 302 DEGS, UBE2C was identified as the gene associated with BRCA survival. The expression of UBE2C was differentially upregulated in BRCA, as verified by TCGA and q RT-PCR. Prognostic analysis revealed that UBE2C served as an independent prognostic factor. High expression of UBE2C was associated with decreased immune infiltration levels of B cells, CD4+ T cells, CD8+ T cells, macrophages, and myeloid dendritic cells in BRCA tissue. The expression of UBE2C in BRCA showed a significant correlation with immune checkpoints genes PDCD1, CD274, and CTLA4 expressions. There was a positive correlation between the expression of UBE2C and the tumor mutational burden and microsatellite instability. GSEA demonstrated that UBE2C expression significantly enriched 786 immune-related gene sets.Conclusions UBE2C expression in BRCA tissues is closely related to the BRCA immune microenvironment and showes predictive values on the survivals and prognosis of BRCA patients and the effecacy of immunotherapy. UBE2C may be an potential immune-related prognostic biomarker for BRCA.
基金National Natural Science Foundation of China(Grant No.81470390)Shanghai Municipal Education Commission-Gaofeng Clinical Medicine Grant Support(Grant No.20152218)+1 种基金Shanghai Sailing Program(Grant No.19YF1431700)Clinical Trial of Xinhua Hospital(Grant No.15LC11)。
文摘Acute fatty liver of pregnancy(AFLP)is an idiopathic disease with acute and critical onset.Although the incidence of AFLP is relatively low,its mortality remains high.When AFLP is complicated with multi-organ dysfunction syndrome(MODS)and severe infection,especially hematogenous infection caused by multidrug-resistant pathogens,treatment becomes extremely difficult with an even lower survival rate.In the present work,we reported a 31-year-old primipara woman who developed AFLP complicated with MODS and severe infection at 37+5 weeks of gestation and received treatment in our hospital.The result of fast blood culture was positive for carbapenem-resistant Klebsiella pneumonia(CRKP).Based on this finding in combination with the results of drug sensitivity test,the quadruple regime using scheme imipenem cilastatin sodium,tigecycline,polymyxin and fosfomycin was implemented,and the patient was successfully cured.Successful treatment of pregnant women with AFLP complicated with MODS and/or hematogenous infection of CRKP can be achieved by using individualized combined antibiotic therapies on the basis of fast blood culture and combined drug sensitivity test.In this case,the patient was in a critical situation.In the treatment process,clinicians,clinical pharmacists and microbiologists should cooperate with each other and discuss the treatment plan together according to the pathophysiological characteristics of the patient,which was one of the key factors for successful treatment of the patient.
文摘Wnt5a is a secreted Wnt ligand that plays a critical role in cellular pathways and inflammatory diseases.The WNT5A gene encodes two protein isoforms,Wnt5a-long and Wnt5a-short,which differ based on different promoter methylation and have distinct functions.However,the mechanisms of the promoter methylation are unclear.Depending on the extent of promoter methylation,Wnt5a exerts both anti-inflammatory and proinflammatory effects in inflammatory diseases,which may be involved in different Wnt5a isoforms.Therefore,the Wnt5a isoforms may be potential diagnostic markers for inflammatory diseases and the mechanisms of the WNT5A gene promoter methylation need to be further investigated.
基金supported by the National Natural Science Foundation of China(31970444,31772497)。
文摘Tachydromia Meigen is a somewhat large genus in the family Empididae with 112 known species distributed worldwide.Ten species were previously known to occur in China.Here the genus Tachydromia is newly recorded from Shaanxi.The following two new species belonging to the Tachydromia arrogans species group are described:Tachydromia basiflava sp.nov.and Tachydromia crassisetosa sp.nov.A key to the species of Tachydromia from China is provided.
基金supported by Key Project of Science-Technology Basic Condition Platform from The Ministry of Science and Technology of the People’s Republic of China(2005DKA21402)
文摘Genus Ptilomyia Coquillett(Diptera: Ephydridae) is reported from the Oriental Region and China for the first time. A new species, P. chinensis sp. nov., is described and illustrated. A key to the Oriental and Palaearctic regions of Ptilomyia is given. The types are kept in the Entomological Museum of China Agricultural University(CAU), Beijing.
