目的研究紫外线照射充氧自体血(U B IO)心脏停搏液冠状动脉间断顺行灌注对体外循环(CPB)中心肌的保护作用。方法将20只健康成年杂种犬(雌雄不拘)随机分为实验组和对照组,每组10只。CPB中实验组采用紫外线照射充氧自体血心脏停搏液间断灌...目的研究紫外线照射充氧自体血(U B IO)心脏停搏液冠状动脉间断顺行灌注对体外循环(CPB)中心肌的保护作用。方法将20只健康成年杂种犬(雌雄不拘)随机分为实验组和对照组,每组10只。CPB中实验组采用紫外线照射充氧自体血心脏停搏液间断灌注;对照组用等量4℃S t.T hom asⅡ号冷晶体液作为心脏停搏液间断灌注,两组其余操作相同。结果开放升主动脉后,实验组冠状静脉窦血清cT n I、CK-M B水平和左心室心肌M DA水平低于对照组(P<0.05),左心室心肌SOD活性和ATP水平高于对照组(P<0.05);心脏自动复跳率明显高于对照组(P<0.01)。心肌超微结构观察显示,实验组左心室心肌纤维结构完整,细胞内线粒体轻度水肿,而对照组心肌纤维部分断裂、溶解,线粒体基质外溢,糖原颗粒减少。结论U B IO血心脏停搏液有较好的心肌保护作用,且能减少心脏停搏液的灌注次数,延长心脏停搏时间;本研究为U B IO血心脏停搏液的临床应用奠定了基础。展开更多
目的探讨无管化经皮肾镜碎石取石术(PCNL)治疗肾和输尿管上段结石的安全性、可行性和禁忌证。方法回顾性研究中山大学附属第三医院·岭南医院泌尿外科2016年11月至2018年5月80例PCNL患者临床资料,已排除脓肾结石患者,根据是否留置...目的探讨无管化经皮肾镜碎石取石术(PCNL)治疗肾和输尿管上段结石的安全性、可行性和禁忌证。方法回顾性研究中山大学附属第三医院·岭南医院泌尿外科2016年11月至2018年5月80例PCNL患者临床资料,已排除脓肾结石患者,根据是否留置肾造瘘管分组:患者术后输尿管内留置双J管,同时留置肾造瘘管40例(传统PCNL组),术后只留置双J管,无留置肾造瘘管40例(无管化PCNL组)。结果两组患者年龄、肾和输尿管结石位置和结石负荷、肾积水直径、总手术时间和结石清除率等差异比较均无统计学意义。患者术后第一天疼痛评分(VAS)试验组比对照组低(3.4±0.9 vs 5.6±1.4),平均住院时间与对照组比较缩短(7.9±1.4 vs 8.8±1.6)d,差异均有统计学意义(P<0.05)。结论无管化PCNL治疗非脓性肾输尿管上段结石安全可行,且可减轻患者术后疼痛,缩短住院时间。展开更多
Diagenetic fluid types of the Cretaceous Bashijiqike formation are restored based on the analysis of petrographic,electron microprobe composition,inclusions homogenization temperature,salinity and vapor composition an...Diagenetic fluid types of the Cretaceous Bashijiqike formation are restored based on the analysis of petrographic,electron microprobe composition,inclusions homogenization temperature,salinity and vapor composition and laser carbon and oxygen isotope of diagenetic mineral,and regional geological background.Diagenetic fluid evolution sequence is analyzed on this basis.The crystalline dolomite cement has a low concerntration of Sr,high concerntration of Mn and higher carbon isotope,showing that the crystalline dolomite is affected by meteoric fresh water,associated with the tectonic uplift of late Cretaceous.Similarδ13CPDB,negative transfer ofδ18OPDB and the differentiation of the concerntration of Fe and Mn indicate that the diagenetic fluid of the vein dolomite cement is homologous with the diagenetic fluid of the crystalline dolomite cement,temperature and depth are the dominant factors of differential precipitation between these two carbonate cements.Anhydrite cements have high concerntration of Na,extremely low concerntration of Fe and Mn contents.Based on these data,anhydrite cements can be thought to be related to the alkaline fluid overlying gypsum-salt layer produced by dehydration.The barite vein has abnormally high concerntration of Sr,ultra-high homogenization temperature and high-density gas hydrocarbon inclusions,which is speculated to be the forward fluid by intrusion of late natural gas.Coexistence of methane inclusions with CO2 gas proves existence of acid water during the accumulation of natural gas in the late stages.Therefore,the alkaline environment and associated diagenesis between the meteoric fresh water in epidiagentic stage and carbonic acid in the late diagenesis have dominated the process of diagenesis and reservoir,the secondary porosity and fracture zone formed by gas accumulation is a favorable play for the exploration of ultra-deep reservoirs.展开更多
文摘目的研究紫外线照射充氧自体血(U B IO)心脏停搏液冠状动脉间断顺行灌注对体外循环(CPB)中心肌的保护作用。方法将20只健康成年杂种犬(雌雄不拘)随机分为实验组和对照组,每组10只。CPB中实验组采用紫外线照射充氧自体血心脏停搏液间断灌注;对照组用等量4℃S t.T hom asⅡ号冷晶体液作为心脏停搏液间断灌注,两组其余操作相同。结果开放升主动脉后,实验组冠状静脉窦血清cT n I、CK-M B水平和左心室心肌M DA水平低于对照组(P<0.05),左心室心肌SOD活性和ATP水平高于对照组(P<0.05);心脏自动复跳率明显高于对照组(P<0.01)。心肌超微结构观察显示,实验组左心室心肌纤维结构完整,细胞内线粒体轻度水肿,而对照组心肌纤维部分断裂、溶解,线粒体基质外溢,糖原颗粒减少。结论U B IO血心脏停搏液有较好的心肌保护作用,且能减少心脏停搏液的灌注次数,延长心脏停搏时间;本研究为U B IO血心脏停搏液的临床应用奠定了基础。
文摘目的探讨无管化经皮肾镜碎石取石术(PCNL)治疗肾和输尿管上段结石的安全性、可行性和禁忌证。方法回顾性研究中山大学附属第三医院·岭南医院泌尿外科2016年11月至2018年5月80例PCNL患者临床资料,已排除脓肾结石患者,根据是否留置肾造瘘管分组:患者术后输尿管内留置双J管,同时留置肾造瘘管40例(传统PCNL组),术后只留置双J管,无留置肾造瘘管40例(无管化PCNL组)。结果两组患者年龄、肾和输尿管结石位置和结石负荷、肾积水直径、总手术时间和结石清除率等差异比较均无统计学意义。患者术后第一天疼痛评分(VAS)试验组比对照组低(3.4±0.9 vs 5.6±1.4),平均住院时间与对照组比较缩短(7.9±1.4 vs 8.8±1.6)d,差异均有统计学意义(P<0.05)。结论无管化PCNL治疗非脓性肾输尿管上段结石安全可行,且可减轻患者术后疼痛,缩短住院时间。
基金Projects(51674211,51534006)supported by the National Natural Science Foundation of China
文摘Diagenetic fluid types of the Cretaceous Bashijiqike formation are restored based on the analysis of petrographic,electron microprobe composition,inclusions homogenization temperature,salinity and vapor composition and laser carbon and oxygen isotope of diagenetic mineral,and regional geological background.Diagenetic fluid evolution sequence is analyzed on this basis.The crystalline dolomite cement has a low concerntration of Sr,high concerntration of Mn and higher carbon isotope,showing that the crystalline dolomite is affected by meteoric fresh water,associated with the tectonic uplift of late Cretaceous.Similarδ13CPDB,negative transfer ofδ18OPDB and the differentiation of the concerntration of Fe and Mn indicate that the diagenetic fluid of the vein dolomite cement is homologous with the diagenetic fluid of the crystalline dolomite cement,temperature and depth are the dominant factors of differential precipitation between these two carbonate cements.Anhydrite cements have high concerntration of Na,extremely low concerntration of Fe and Mn contents.Based on these data,anhydrite cements can be thought to be related to the alkaline fluid overlying gypsum-salt layer produced by dehydration.The barite vein has abnormally high concerntration of Sr,ultra-high homogenization temperature and high-density gas hydrocarbon inclusions,which is speculated to be the forward fluid by intrusion of late natural gas.Coexistence of methane inclusions with CO2 gas proves existence of acid water during the accumulation of natural gas in the late stages.Therefore,the alkaline environment and associated diagenesis between the meteoric fresh water in epidiagentic stage and carbonic acid in the late diagenesis have dominated the process of diagenesis and reservoir,the secondary porosity and fracture zone formed by gas accumulation is a favorable play for the exploration of ultra-deep reservoirs.
