Two Escherichia coli Saccharomyces cerevisiae shuttle vectors containing a resistance marker to geneticin (G418) are constructed. Both vectors contain a kanamycin resistant marker (KanMX4) module coding aminoglyc...Two Escherichia coli Saccharomyces cerevisiae shuttle vectors containing a resistance marker to geneticin (G418) are constructed. Both vectors contain a kanamycin resistant marker (KanMX4) module coding aminoglycoside 3' phosphotransferase (APH) that renders E. coli resistant to kanamycin and S. cerevisiae to geneticin. These vectors overcome the shortage of the conventional yeast vectors bearing HIS3, TRP1, LEU2, and URA3 modules as selection markers, which require hosts to be auxotrophic. Green fluorescent protein (GFP) is used as the reporter to examine the functions of the vectors. The vectors are powerful tools for the convenient cloning and controlled expression of genes in most S. cerevisiae strains.展开更多
Pseudomonas flava HBE06 isolated from oil contaminated soil was found to produce polyesters consisting of medium chain length polyhydroxyalkanoates (mcl PHA). The strain mainly synthesized PHA containing 3 hydroxyoc...Pseudomonas flava HBE06 isolated from oil contaminated soil was found to produce polyesters consisting of medium chain length polyhydroxyalkanoates (mcl PHA). The strain mainly synthesized PHA containing 3 hydroxyoctanoate (C 8 or HO), 3 hydroxynonanoate (C 9 or HN), 3 hydroxydecanoate (C 10 or HD), and 3 hydroxyunidecanoate (C 11 or HUD) as monomers when grown on various substrates. It was found that the monomer ratio (especially C 10 /C 8 or C 11 /C 9) was related to the PHA biosynthesis pathway. When PHA was synthesized via the de novo fatty acid pathway, the molar ratio of C 10 /C 8 was greater than 2. If PHA was synthesized from β oxidation of fatty acids, C 10 /C 8 was less than 1. Pseudomonas stutzeri 1317 is another mcl PHA synthesizing bacteria whose C 10 /C 8 ratio is also related to the synthesis pathway. When the two synthesis pathways were used together, the C 10 /C 8 ratio could be adjusted according to the ratio of the mixed substrates. 展开更多
文摘Two Escherichia coli Saccharomyces cerevisiae shuttle vectors containing a resistance marker to geneticin (G418) are constructed. Both vectors contain a kanamycin resistant marker (KanMX4) module coding aminoglycoside 3' phosphotransferase (APH) that renders E. coli resistant to kanamycin and S. cerevisiae to geneticin. These vectors overcome the shortage of the conventional yeast vectors bearing HIS3, TRP1, LEU2, and URA3 modules as selection markers, which require hosts to be auxotrophic. Green fluorescent protein (GFP) is used as the reporter to examine the functions of the vectors. The vectors are powerful tools for the convenient cloning and controlled expression of genes in most S. cerevisiae strains.
文摘Pseudomonas flava HBE06 isolated from oil contaminated soil was found to produce polyesters consisting of medium chain length polyhydroxyalkanoates (mcl PHA). The strain mainly synthesized PHA containing 3 hydroxyoctanoate (C 8 or HO), 3 hydroxynonanoate (C 9 or HN), 3 hydroxydecanoate (C 10 or HD), and 3 hydroxyunidecanoate (C 11 or HUD) as monomers when grown on various substrates. It was found that the monomer ratio (especially C 10 /C 8 or C 11 /C 9) was related to the PHA biosynthesis pathway. When PHA was synthesized via the de novo fatty acid pathway, the molar ratio of C 10 /C 8 was greater than 2. If PHA was synthesized from β oxidation of fatty acids, C 10 /C 8 was less than 1. Pseudomonas stutzeri 1317 is another mcl PHA synthesizing bacteria whose C 10 /C 8 ratio is also related to the synthesis pathway. When the two synthesis pathways were used together, the C 10 /C 8 ratio could be adjusted according to the ratio of the mixed substrates.
