Three methods to determine The molecular weight of a protein electroblotted onto PVDF or NC membrane have been investigated in this paper The "Pseudo-dissolution Method"(direct method) is able to directly an...Three methods to determine The molecular weight of a protein electroblotted onto PVDF or NC membrane have been investigated in this paper The "Pseudo-dissolution Method"(direct method) is able to directly analyze proteins on membrane provided a good staining-destaining process and a gentle transferring strategy were carried out. The "Extraction Method" is suitable for various kinds of membranes, its reproducibility, sensitivity, and resolution are high. The"Dissolution-Precipitation Method" is the best among these methods. The quality of MALDI-TOF spectrum using this simple method is the same as The routine strategy of MALDI.展开更多
Amoxicillin is a kind of β-lactam antibacterial.It has been widely used in clinic.Recently,some products of amoxicillin were found to be unqualified in content and acidity.In this paper,the quality of amoxicillin wer...Amoxicillin is a kind of β-lactam antibacterial.It has been widely used in clinic.Recently,some products of amoxicillin were found to be unqualified in content and acidity.In this paper,the quality of amoxicillin were firstly analyzed by using FT-ICR-MS and FT-ICR-MS/MS method by comparing the accurate relative molecular mass of amoxicillin fragmentation and its accurate mass obtained.展开更多
文摘Three methods to determine The molecular weight of a protein electroblotted onto PVDF or NC membrane have been investigated in this paper The "Pseudo-dissolution Method"(direct method) is able to directly analyze proteins on membrane provided a good staining-destaining process and a gentle transferring strategy were carried out. The "Extraction Method" is suitable for various kinds of membranes, its reproducibility, sensitivity, and resolution are high. The"Dissolution-Precipitation Method" is the best among these methods. The quality of MALDI-TOF spectrum using this simple method is the same as The routine strategy of MALDI.
文摘Amoxicillin is a kind of β-lactam antibacterial.It has been widely used in clinic.Recently,some products of amoxicillin were found to be unqualified in content and acidity.In this paper,the quality of amoxicillin were firstly analyzed by using FT-ICR-MS and FT-ICR-MS/MS method by comparing the accurate relative molecular mass of amoxicillin fragmentation and its accurate mass obtained.
