一串红(Salvia splendens)株型突变体BN35-1分枝能力强,不必摘心,株型自然成球。本研究利用AFLP(amplified fragment length polymorphism)分子标记技术对其进行分析。从21对引物组合中筛选出6对多态性高的引物组合对突变体BN35-1、野生...一串红(Salvia splendens)株型突变体BN35-1分枝能力强,不必摘心,株型自然成球。本研究利用AFLP(amplified fragment length polymorphism)分子标记技术对其进行分析。从21对引物组合中筛选出6对多态性高的引物组合对突变体BN35-1、野生型BN35及4个商品种进行亲缘关系分析以确定BN35-1与BN35亲缘关系。另外用21对供试引物组合单独对突变体及原种进行多态性分析以寻找差异条带。结果表明,BN35-1与BN35亲缘关系最近,相似系数为0.986 1,远高于其他商品种、BN35与商品种以及商品种间的相似性。在21对供试引物组合中,12对引物检测出BN35-1与BN35基因组DNA共52处基因座位位点存在差异,相应的特异条带是控制株型突变或与控制株型突变基因相连锁的侯选基因片段,深入研究工作有待进一步进行。展开更多
Twenty-third collections of Salvia spp.in Beijing were analyzed phylogenetically by AFLP technique.6 primer pairs with good repetition and highly polymorphic bands from 21 pairs were selected to amplify the genomic DN...Twenty-third collections of Salvia spp.in Beijing were analyzed phylogenetically by AFLP technique.6 primer pairs with good repetition and highly polymorphic bands from 21 pairs were selected to amplify the genomic DNA.All of 367 AFLP bands were obtained,in which 359(97.82%)were polymorphic markers.At a genetic distance of 0.32,the collections could be clustered into 7 species i.e.S.splendens,S.farinacea,S.coccinea,S.plebeia,S.miltiorrhiza,S.umbratica and S.officinalis.The respective genetic relationship be...展开更多
基金Supported by Beijing Municipal Commission of Education Project(KM200910020014)~~
文摘Twenty-third collections of Salvia spp.in Beijing were analyzed phylogenetically by AFLP technique.6 primer pairs with good repetition and highly polymorphic bands from 21 pairs were selected to amplify the genomic DNA.All of 367 AFLP bands were obtained,in which 359(97.82%)were polymorphic markers.At a genetic distance of 0.32,the collections could be clustered into 7 species i.e.S.splendens,S.farinacea,S.coccinea,S.plebeia,S.miltiorrhiza,S.umbratica and S.officinalis.The respective genetic relationship be...
