In this paper,genetic diversity of intraspecies, and genetic relationship of interspecies in Epinephelus spp.（E.merra,E.fario,E.awoara,E.akaara and E.septemfasciatus） were assessed by using mitochondrial DNA restric...In this paper,genetic diversity of intraspecies, and genetic relationship of interspecies in Epinephelus spp.（E.merra,E.fario,E.awoara,E.akaara and E.septemfasciatus） were assessed by using mitochondrial DNA restriction fragment length polymorphisms（mtDNA RFLPs）.The samples were collected from the coastal area of Zhanjiang,Guangdong province.MtDNA was extracted from the fresh liver tissue by applying a difference centrifugation procedures.Using 17 restriction enzymes with 5-or 6-bp recognition sites,the purified mtDNA was cleaved by single enzymes.These enzymes included BamH Ⅰ,Bgl Ⅰ,Bgl[WTB1] Ⅱ,Dra Ⅰ,EcoR Ⅰ,EcoR Ⅴ,Hind Ⅲ,Kpn Ⅰ,Mlu Ⅰ,Pst Ⅰ,Pvu Ⅱ,Sal Ⅰ,Sca Ⅰ,Sma Ⅰ,Sty Ⅰ,Xba Ⅰ and Xho Ⅰ.The phylogenetic analysis was done using the Neighbor-joining（NJ） method and Unweighted pair-group method with arithmetic mean（UPGMA） method.Genetic diversity indices such as haplotype diversity（h）,average genetic distance between haplotypes（P） and nucleotide diversity（π） were calculated using Nei and Li’s segment method to quantify the genetic diversity within species.There were 8,5,8,5 and 2 haplotypes detected within E.merra,E.fario,E.awoara,E.akaara and E.septemfasciatus,respectively.The haplotype diversity（h） was 0.8943,0.6186,0.9242,0.6927 and 0.1820,respectively.The average genetic distance between haplotypes（P） was 0.62%±0.31%,0.64%±0.37%,1.12%±0.55%,0.72%±0.42% and 0.45%,respectively.And the nucleotide diversity（π） was 0.22%,0.13%,0.46%,0.17% and 0.04%,respectively.The wild groupers in the Zhanjiang Coastal Area exhibited a relative higher level of genetic diversity.The net genetic distance between species(P[WTB1]net) was 0.0694（E.merra-E.fario）,0.1337（E.merra-E.awoara）,0.1090（E.merra-E.akaara）,0.1286（E.merra-E.septemfasciatus）,0.1590（E.farioE.awoara）,0.0825（E.fario-E.akaara）,0.1153（E.fario-E.septemfasciatus）,0.1131（E.awoara-E.akaara）,0.0724（E.awoara-E.septemfasciatus）and 0.1336（E.akaara-E.septemfasciatus�展开更多
文摘In this paper,genetic diversity of intraspecies, and genetic relationship of interspecies in Epinephelus spp.（E.merra,E.fario,E.awoara,E.akaara and E.septemfasciatus） were assessed by using mitochondrial DNA restriction fragment length polymorphisms（mtDNA RFLPs）.The samples were collected from the coastal area of Zhanjiang,Guangdong province.MtDNA was extracted from the fresh liver tissue by applying a difference centrifugation procedures.Using 17 restriction enzymes with 5-or 6-bp recognition sites,the purified mtDNA was cleaved by single enzymes.These enzymes included BamH Ⅰ,Bgl Ⅰ,Bgl[WTB1] Ⅱ,Dra Ⅰ,EcoR Ⅰ,EcoR Ⅴ,Hind Ⅲ,Kpn Ⅰ,Mlu Ⅰ,Pst Ⅰ,Pvu Ⅱ,Sal Ⅰ,Sca Ⅰ,Sma Ⅰ,Sty Ⅰ,Xba Ⅰ and Xho Ⅰ.The phylogenetic analysis was done using the Neighbor-joining（NJ） method and Unweighted pair-group method with arithmetic mean（UPGMA） method.Genetic diversity indices such as haplotype diversity（h）,average genetic distance between haplotypes（P） and nucleotide diversity（π） were calculated using Nei and Li’s segment method to quantify the genetic diversity within species.There were 8,5,8,5 and 2 haplotypes detected within E.merra,E.fario,E.awoara,E.akaara and E.septemfasciatus,respectively.The haplotype diversity（h） was 0.8943,0.6186,0.9242,0.6927 and 0.1820,respectively.The average genetic distance between haplotypes（P） was 0.62%±0.31%,0.64%±0.37%,1.12%±0.55%,0.72%±0.42% and 0.45%,respectively.And the nucleotide diversity（π） was 0.22%,0.13%,0.46%,0.17% and 0.04%,respectively.The wild groupers in the Zhanjiang Coastal Area exhibited a relative higher level of genetic diversity.The net genetic distance between species(P[WTB1]net) was 0.0694（E.merra-E.fario）,0.1337（E.merra-E.awoara）,0.1090（E.merra-E.akaara）,0.1286（E.merra-E.septemfasciatus）,0.1590（E.farioE.awoara）,0.0825（E.fario-E.akaara）,0.1153（E.fario-E.septemfasciatus）,0.1131（E.awoara-E.akaara）,0.0724（E.awoara-E.septemfasciatus）and 0.1336（E.akaara-E.septemfasciatus�
