To decipher the organizational logic of complex brain circuits,it is important to chart long-distance pathways while preserving micron-level accuracy of local network.However,mapping the neuronal projections with indi...To decipher the organizational logic of complex brain circuits,it is important to chart long-distance pathways while preserving micron-level accuracy of local network.However,mapping the neuronal projections with individual-axon resolution in the large and complex primate brain is still challenging.Herein,we describe a highly efficient pipeline for three-dimensional mapping of the entire macaque brain with subcellular resolution.The pipeline includes a novel poly-N-acryloyl glycinamide(PNAGA)-based embedding method for long-term structure and fluorescence preservation,high-resolution and rapid whole-brain optical imaging,and image post-processing.The cytoarchitectonic information of the entire macaque brain was acquired with a voxel size of 0.32μm×0.32μm×10μm,showing its anatomical structure with cell distribution,density,and shape.Furthermore,thanks to viral labeling,individual long-distance projection axons from the frontal cortex were for the first time reconstructed across the entire brain hemisphere with a voxel size of 0.65μm×0.65μm×3μm.Our results show that individual cortical axons originating from the prefrontal cortex simultaneously target multiple brain regions,including the visual cortex,striatum,thalamus,and midbrain.This pipeline provides an efficient method for cellular and circuitry investigation of the whole macaque brain with individual-axon resolution,and can shed light on brain function and disorders.展开更多
A reflection-mode photoacoustic microscope（PAM） for rat brain imaging in vivo is constructed.A pulsed laser is used as an excitation source,and a focused ultrasound transducer is adopted to collect the photoacoustic...A reflection-mode photoacoustic microscope（PAM） for rat brain imaging in vivo is constructed.A pulsed laser is used as an excitation source,and a focused ultrasound transducer is adopted to collect the photoacoustic signal.Raster scanning is applied to acquire three-dimensional（3D） data.The obtained measurements of the lateral and axial resolutions of the microscope are 45 and 15μm,respectively.The imaging depth in the chicken breast tissue is 3.1 mm at a signal-to-noise ratio（SNR） of 20 dB without any signal averaging.The imaging speed is 30 A-line/s.Experimental results in vivo demonstrate the capability of 3D imaging of the brain vessels of the rat after removing the skull.展开更多
A fluorescence molecular tomography system for in vivo tumor imaging is developed using a 748-nm continuous wave diode laser as an excitation source.A high sensitivity cooled charge-coupled device （CCD） camera with ...A fluorescence molecular tomography system for in vivo tumor imaging is developed using a 748-nm continuous wave diode laser as an excitation source.A high sensitivity cooled charge-coupled device （CCD） camera with excitation and emission filters is utilized to obtain the excitation and fluorescence images.The laser beam performs fast raster scanning using a dual-axis galvanometric scanner.The accuracy of the laser spot position at the source window is within ±200 μm.Based on the phantom experimental results,the spatial resolution is less than 1.7 mm,and the relative quantitation error is about 10%.In vivo imaging of a tumor-bearing nude mouse tagged with near-infrared dye demonstrates the feasibility of the system.展开更多
Combining photoacoustic（PA） imaging with laser speckle（LS） imaging（LSI） can simultaneously determine total hemoglobin concentration（HbT）,hemoglobin oxygen saturation（SO2）,and blood flow rates.Thus,the co-reg...Combining photoacoustic（PA） imaging with laser speckle（LS） imaging（LSI） can simultaneously determine total hemoglobin concentration（HbT）,hemoglobin oxygen saturation（SO2）,and blood flow rates.Thus,the co-registration of PA and LS images is important in physiological studies and pathological diagnosis.This letter presents a co-registration algorithm combining mutual information with the maximum betweenclass variance segmentation method（Otsu method）.The mutual information and Otsu method are used to provide the registration measure criterion and image feature recognition,respectively.The evaluation results show that the registration function possesses a single maximum peak and high smoothness across the global co-registration district,indicating a robust behavior.Moreover,this method has good registration accuracy,and the fusion result simultaneously visualizes the separate functional information of two kinds of images.展开更多
基金supported by the National Basic Research Program of China(973 Project2015CB755602)+3 种基金the National Natural Science Foundation of China(61721092,61890953,31871088,and 81871082)Key-Area Research and Development Program of Guangdong Province(2019B030335001)CAMS Innovation Fund for Medical Sciences(2019-I2M-5-014)the Director Fund of Wuhan National Laboratory for Optoelectronics。
文摘To decipher the organizational logic of complex brain circuits,it is important to chart long-distance pathways while preserving micron-level accuracy of local network.However,mapping the neuronal projections with individual-axon resolution in the large and complex primate brain is still challenging.Herein,we describe a highly efficient pipeline for three-dimensional mapping of the entire macaque brain with subcellular resolution.The pipeline includes a novel poly-N-acryloyl glycinamide(PNAGA)-based embedding method for long-term structure and fluorescence preservation,high-resolution and rapid whole-brain optical imaging,and image post-processing.The cytoarchitectonic information of the entire macaque brain was acquired with a voxel size of 0.32μm×0.32μm×10μm,showing its anatomical structure with cell distribution,density,and shape.Furthermore,thanks to viral labeling,individual long-distance projection axons from the frontal cortex were for the first time reconstructed across the entire brain hemisphere with a voxel size of 0.65μm×0.65μm×3μm.Our results show that individual cortical axons originating from the prefrontal cortex simultaneously target multiple brain regions,including the visual cortex,striatum,thalamus,and midbrain.This pipeline provides an efficient method for cellular and circuitry investigation of the whole macaque brain with individual-axon resolution,and can shed light on brain function and disorders.
基金supported by the National Natural Science Foundation of China(No.60828009)the Programme of Introducing Talents of Discipline to Universities (111 Project).
文摘A reflection-mode photoacoustic microscope（PAM） for rat brain imaging in vivo is constructed.A pulsed laser is used as an excitation source,and a focused ultrasound transducer is adopted to collect the photoacoustic signal.Raster scanning is applied to acquire three-dimensional（3D） data.The obtained measurements of the lateral and axial resolutions of the microscope are 45 and 15μm,respectively.The imaging depth in the chicken breast tissue is 3.1 mm at a signal-to-noise ratio（SNR） of 20 dB without any signal averaging.The imaging speed is 30 A-line/s.Experimental results in vivo demonstrate the capability of 3D imaging of the brain vessels of the rat after removing the skull.
基金supported by the National High-Tech Research and Development ("863") Program of China under Grant No. 2006AA020801
文摘A fluorescence molecular tomography system for in vivo tumor imaging is developed using a 748-nm continuous wave diode laser as an excitation source.A high sensitivity cooled charge-coupled device （CCD） camera with excitation and emission filters is utilized to obtain the excitation and fluorescence images.The laser beam performs fast raster scanning using a dual-axis galvanometric scanner.The accuracy of the laser spot position at the source window is within ±200 μm.Based on the phantom experimental results,the spatial resolution is less than 1.7 mm,and the relative quantitation error is about 10%.In vivo imaging of a tumor-bearing nude mouse tagged with near-infrared dye demonstrates the feasibility of the system.
基金supported by the National Major Scientific Research Program of China (No. 2011CB910401)the Science Fund for Creative Research Group of China(No. 61121004)+1 种基金the National Natural Science Foundation of China (No. 61078072)the Fundamental Research Funds for the Central Universities, Huazhong University of Science and Technology (No. 2010MS111)
文摘Combining photoacoustic（PA） imaging with laser speckle（LS） imaging（LSI） can simultaneously determine total hemoglobin concentration（HbT）,hemoglobin oxygen saturation（SO2）,and blood flow rates.Thus,the co-registration of PA and LS images is important in physiological studies and pathological diagnosis.This letter presents a co-registration algorithm combining mutual information with the maximum betweenclass variance segmentation method（Otsu method）.The mutual information and Otsu method are used to provide the registration measure criterion and image feature recognition,respectively.The evaluation results show that the registration function possesses a single maximum peak and high smoothness across the global co-registration district,indicating a robust behavior.Moreover,this method has good registration accuracy,and the fusion result simultaneously visualizes the separate functional information of two kinds of images.
