Aphid is a group of small insects in the Homoptera.There are approximately 4 000 aphid species all over the world.Many of them attack important agricultural crops and therefore have economic importance.However,some sp...Aphid is a group of small insects in the Homoptera.There are approximately 4 000 aphid species all over the world.Many of them attack important agricultural crops and therefore have economic importance.However,some species such as Chinese gallnut aphids are beneficial for human beings.Most aphids display complex life cycles with alternation of sexual and asexual generations and host plant alternation.Molecular genetic markers have good stabilities,high variability and mostly are free from environmental influences.So they were very suitable for aphid research and could tackle some problems which the traditional taxonomical methods could not solve.The paper introduced a simple and effective method that could extract genomic DNA from a single aphid individual and could be used for the research of molecular genetic marker such as RAPD,RFLP and DNA sequence determination.展开更多
The vertical distribution characteristics of Monochamus alternatus on the trucks of infested Pinus yunnanensis were investigated both in field and laboratory.The results showed that the quantity distribution of ovipos...The vertical distribution characteristics of Monochamus alternatus on the trucks of infested Pinus yunnanensis were investigated both in field and laboratory.The results showed that the quantity distribution of oviposition scar in trunk was lower part > middle part > upper part;and the density distribution of oviposition scar had not significant difference(P>0.05).The quantity distribution of entrance hole in trunk was middle part > lower part > upper part;and the density distribution of entrance hole was upper part > middle part > lower part.The quantity distribution of exit hole in trunk was middle part > upper part > lower part;and the density distribution of exit hole was upper part > middle part > lower part.There were positive correlations between the quantities of oviposition scar or entrance hole in lower part of trunk and the entrance hole or exit hole in whole trunk,respectively.The linear regression equations of the quantity of oviposition scar in lower part(x1),the entrance hole in lower part(x2) and the quantity of oviposition scar in whole trunk(y1),the entrance hole in whole trunk(y2),the exit hole in whole trunk(y3) are y1=7.117+2.283 x1,y2=24.220+0.701 x1,y3=8.794+0.434 x1,y2=28.805+2.211 x2,y3=20.367+0.778 x2.All partial correlations had reached the very signiticant level.展开更多
文摘Aphid is a group of small insects in the Homoptera.There are approximately 4 000 aphid species all over the world.Many of them attack important agricultural crops and therefore have economic importance.However,some species such as Chinese gallnut aphids are beneficial for human beings.Most aphids display complex life cycles with alternation of sexual and asexual generations and host plant alternation.Molecular genetic markers have good stabilities,high variability and mostly are free from environmental influences.So they were very suitable for aphid research and could tackle some problems which the traditional taxonomical methods could not solve.The paper introduced a simple and effective method that could extract genomic DNA from a single aphid individual and could be used for the research of molecular genetic marker such as RAPD,RFLP and DNA sequence determination.
文摘The vertical distribution characteristics of Monochamus alternatus on the trucks of infested Pinus yunnanensis were investigated both in field and laboratory.The results showed that the quantity distribution of oviposition scar in trunk was lower part > middle part > upper part;and the density distribution of oviposition scar had not significant difference(P>0.05).The quantity distribution of entrance hole in trunk was middle part > lower part > upper part;and the density distribution of entrance hole was upper part > middle part > lower part.The quantity distribution of exit hole in trunk was middle part > upper part > lower part;and the density distribution of exit hole was upper part > middle part > lower part.There were positive correlations between the quantities of oviposition scar or entrance hole in lower part of trunk and the entrance hole or exit hole in whole trunk,respectively.The linear regression equations of the quantity of oviposition scar in lower part(x1),the entrance hole in lower part(x2) and the quantity of oviposition scar in whole trunk(y1),the entrance hole in whole trunk(y2),the exit hole in whole trunk(y3) are y1=7.117+2.283 x1,y2=24.220+0.701 x1,y3=8.794+0.434 x1,y2=28.805+2.211 x2,y3=20.367+0.778 x2.All partial correlations had reached the very signiticant level.
