To provide materials used in investigating the relationship between amino acid compositions of silk-like protein, structure, and functions, especially the biological functions, the motif genes encoding the silk fibroi...To provide materials used in investigating the relationship between amino acid compositions of silk-like protein, structure, and functions, especially the biological functions, the motif genes encoding the silk fibroin amorphous domain, SGFGPVANGGSGEASSESDFGSSGFGPVANASSGEASSESDFAG(F) were designed and extended using a "head-to-tail" construction strategy. The designed genes were cloned into PSLFA1180FA and multimerized to form structures containing a two-timer, a four-timer, an eight-timer, and a twelve-timer. All the resulting plasmids were digested using the restriction enzyme BamHI and the double-enzymes BglII/HindIII. Restriction enzyme analysis and DNA sequencing revealed the motif was successfully cloned into PSLFA1180FA and multimerized to form a twelve-timer without gene deletion or mutation.展开更多
目的探讨动力髋螺钉(dynamic hip screw DHS)手术治疗股骨转子间骨折的疗效。方法采用DHS治疗股骨转子间骨折26例,Ⅳ型骨折术中加用防旋拉加力螺钉。结果25例获得随访,骨折愈合时间5-10月,无切口感染,无髋膝关节功能障碍,2例发生轻度髋...目的探讨动力髋螺钉(dynamic hip screw DHS)手术治疗股骨转子间骨折的疗效。方法采用DHS治疗股骨转子间骨折26例,Ⅳ型骨折术中加用防旋拉加力螺钉。结果25例获得随访,骨折愈合时间5-10月,无切口感染,无髋膝关节功能障碍,2例发生轻度髋内翻。结论DHS手术治疗股骨转子间骨折具有固定牢固,断端加压,骨折愈合快等特点,加用防旋拉加力螺钉后可有效防止骨折端的旋转,畸形发生率低。展开更多
基金National Natural Science Foundation of China(No. 51173125)Natural Science Foundations of Jiangsu Province of China(No. BK2010253,No. BK2012633)+2 种基金College Natural Science Research Project of Jiangsu Province of China(No. 12KJA43004)Science and Technology Plan Foundation of Suzhou of China(No. ZXS2012002)Priority Academic Program Development of Jiangsu Higher Education Institutions,China
文摘To provide materials used in investigating the relationship between amino acid compositions of silk-like protein, structure, and functions, especially the biological functions, the motif genes encoding the silk fibroin amorphous domain, SGFGPVANGGSGEASSESDFGSSGFGPVANASSGEASSESDFAG(F) were designed and extended using a "head-to-tail" construction strategy. The designed genes were cloned into PSLFA1180FA and multimerized to form structures containing a two-timer, a four-timer, an eight-timer, and a twelve-timer. All the resulting plasmids were digested using the restriction enzyme BamHI and the double-enzymes BglII/HindIII. Restriction enzyme analysis and DNA sequencing revealed the motif was successfully cloned into PSLFA1180FA and multimerized to form a twelve-timer without gene deletion or mutation.
文摘目的探讨动力髋螺钉(dynamic hip screw DHS)手术治疗股骨转子间骨折的疗效。方法采用DHS治疗股骨转子间骨折26例,Ⅳ型骨折术中加用防旋拉加力螺钉。结果25例获得随访,骨折愈合时间5-10月,无切口感染,无髋膝关节功能障碍,2例发生轻度髋内翻。结论DHS手术治疗股骨转子间骨折具有固定牢固,断端加压,骨折愈合快等特点,加用防旋拉加力螺钉后可有效防止骨折端的旋转,畸形发生率低。
