The genetic fingerprints of Chinese wildrye ( Aneurolepidium chinensis (Trin.) Kitag) were constructed by Southern blot analysis of Ase Ⅰ_, Dra Ⅰ_, Eco RⅠ_, Hin dⅢ_, Nco Ⅰ_, Mob Ⅰ_, Rsa Ⅰ_, Pst Ⅰ_, Taq Ⅰ_ dig...The genetic fingerprints of Chinese wildrye ( Aneurolepidium chinensis (Trin.) Kitag) were constructed by Southern blot analysis of Ase Ⅰ_, Dra Ⅰ_, Eco RⅠ_, Hin dⅢ_, Nco Ⅰ_, Mob Ⅰ_, Rsa Ⅰ_, Pst Ⅰ_, Taq Ⅰ_ digested genomic DNA probed with synthesized oligonucleotide,(CT) 8,(GCTA) 4,(GGAT) 4 or (GACA) 4 .The difference between “Jisheng 4" and Chinese wildrye was shown by RFLP analysis of Dra Ⅰ_ or Rsa Ⅰ_ digested genomic DNAs probed with (GCTA) 4 .DNA fragments were obtained by PCR performed with genomic DNA of “Jisheng 4" as a template and (CT) 8,(GCTA) 4,(GGAT) 4 or (GACA) 4 as primer. Five size 2325,1455, 876,774 and 299 bp fragments were amplified when (GGAT) 4 was used, indicating satisfied results were obtained.展开更多
文摘The genetic fingerprints of Chinese wildrye ( Aneurolepidium chinensis (Trin.) Kitag) were constructed by Southern blot analysis of Ase Ⅰ_, Dra Ⅰ_, Eco RⅠ_, Hin dⅢ_, Nco Ⅰ_, Mob Ⅰ_, Rsa Ⅰ_, Pst Ⅰ_, Taq Ⅰ_ digested genomic DNA probed with synthesized oligonucleotide,(CT) 8,(GCTA) 4,(GGAT) 4 or (GACA) 4 .The difference between “Jisheng 4" and Chinese wildrye was shown by RFLP analysis of Dra Ⅰ_ or Rsa Ⅰ_ digested genomic DNAs probed with (GCTA) 4 .DNA fragments were obtained by PCR performed with genomic DNA of “Jisheng 4" as a template and (CT) 8,(GCTA) 4,(GGAT) 4 or (GACA) 4 as primer. Five size 2325,1455, 876,774 and 299 bp fragments were amplified when (GGAT) 4 was used, indicating satisfied results were obtained.
