背根节(dorsal root ganglion,DRG)损伤或炎症可导致DRG神经元兴奋性异常增强和痛觉过敏。我们近期研究显示,长期慢性在体压迫(chronic compression of DRG,CCD)或急性离体分离(acute dissociation of DRG,ADD)背根节导致的神经元兴奋...背根节(dorsal root ganglion,DRG)损伤或炎症可导致DRG神经元兴奋性异常增强和痛觉过敏。我们近期研究显示,长期慢性在体压迫(chronic compression of DRG,CCD)或急性离体分离(acute dissociation of DRG,ADD)背根节导致的神经元兴奋性异常增强和痛觉过敏受环鸟苷酸(cGMP)-蛋白激酶G(PKG)信号通路活动的调控。本研究采用大鼠CCD模型和ADD模型,直接在DRG上检测cGMP浓度和PKG mRNA及其蛋白质的表达,进一步证明了cGMP-PKG信号通路活动在CCD和ADD DRG所致神经元兴奋性异常增强和痛觉过敏中的重要作用。酶联免疫吸附测定(ELISA)和逆转录聚合酶链反应(RT-PCR)的实验结果显示,CCD或ADD明显增高DRG内的cGMP浓度,上调I型PKG mRNA和PKG蛋白质表达。电生理膜片钳全细胞记录结果显示,CCD和ADD显著增强伤害特异性DRG细胞的兴奋性及其对cGMP-PKG信号通路激动剂的反应强度。增强的细胞兴奋性可以被cGMP-PKG通路阻断剂所抑制。在体压迫DRG的椎间孔内注射cGMP-PKG抑制剂显著减轻痛觉过敏。以上研究结果表明,CCD和ADD可以激活DRG细胞内的cGMP-PKG信号通路,而损伤的DRG细胞的超兴奋性和痛觉过敏的维持则需要cGMP-PKG信号通路处于持续的激活状态。展开更多
Metadata is one of the six key technologies of Digital Earth,which is also an important aspect in buildingand implementing the Digital City. Based on the technologies and standards of Web Services ,the author came up ...Metadata is one of the six key technologies of Digital Earth,which is also an important aspect in buildingand implementing the Digital City. Based on the technologies and standards of Web Services ,the author came up witha metadata services architecture in the distributed heterogeneous network environment of Digital City. In this paper,the metadata services architecture is described and explained in detail,including the related standards,technologies,that,based on the Web service,to build the metadata service architecture will be practically meaningful for organizing,managing and sharing the information resources in Digital City.展开更多
文摘Metadata is one of the six key technologies of Digital Earth,which is also an important aspect in buildingand implementing the Digital City. Based on the technologies and standards of Web Services ,the author came up witha metadata services architecture in the distributed heterogeneous network environment of Digital City. In this paper,the metadata services architecture is described and explained in detail,including the related standards,technologies,that,based on the Web service,to build the metadata service architecture will be practically meaningful for organizing,managing and sharing the information resources in Digital City.
