Protoplasts were isolated from embryogenic calli derived from immature embryos ofwheat (Triticum aestivum L. cv. Jinan 177). The protoplasts were cultured in NMB mediumsupplemented with 1mg/L 2,4- D and 500mg/l casein...Protoplasts were isolated from embryogenic calli derived from immature embryos ofwheat (Triticum aestivum L. cv. Jinan 177). The protoplasts were cultured in NMB mediumsupplemented with 1mg/L 2,4- D and 500mg/l casein hydrolysate (CH). The regenerated cellsfrom protoplasts divided to form somatic embryos directly. The somatic embryos grown to1.5- 2 mm in size directly developed into complete plants on solid MB medium without hor-mones.展开更多
Wheat is one of the most important agronomic crops in the world. The regeneration of plants is an important prerequisite for the improvement in genetic manipulation. Protoplast culture of wheat has been proved extreme...Wheat is one of the most important agronomic crops in the world. The regeneration of plants is an important prerequisite for the improvement in genetic manipulation. Protoplast culture of wheat has been proved extremely difficult and intense efforts are making for it. Recently, there have been reports on plantlets formed from wheat protoplasts. However,展开更多
文摘Protoplasts were isolated from embryogenic calli derived from immature embryos ofwheat (Triticum aestivum L. cv. Jinan 177). The protoplasts were cultured in NMB mediumsupplemented with 1mg/L 2,4- D and 500mg/l casein hydrolysate (CH). The regenerated cellsfrom protoplasts divided to form somatic embryos directly. The somatic embryos grown to1.5- 2 mm in size directly developed into complete plants on solid MB medium without hor-mones.
文摘Wheat is one of the most important agronomic crops in the world. The regeneration of plants is an important prerequisite for the improvement in genetic manipulation. Protoplast culture of wheat has been proved extremely difficult and intense efforts are making for it. Recently, there have been reports on plantlets formed from wheat protoplasts. However,
