目的:基于核磁共振氢谱技术(1 H NMR)观察电针"内关"穴预处理对心肌缺血再灌注损伤(MIRI)大鼠血清代谢物及其代谢通路的影响,从代谢组学角度探讨电针预处理对MIRI大鼠产生保护作用的机制。方法:雄性SD大鼠48只随机分为正常组...目的:基于核磁共振氢谱技术(1 H NMR)观察电针"内关"穴预处理对心肌缺血再灌注损伤(MIRI)大鼠血清代谢物及其代谢通路的影响,从代谢组学角度探讨电针预处理对MIRI大鼠产生保护作用的机制。方法:雄性SD大鼠48只随机分为正常组、模型组、电针内关组(内关组)、电针合谷组(合谷组),每组12只。采用结扎冠状动脉左前降支40min后恢复血流1h制备MIRI大鼠模型。两电针组造模前每天予电针相应穴位30min,连续7d。模型制备成功后,收集各组大鼠血清,利用~1 H NMR进行代谢物检测,利用最小二乘分析、正交偏最小二乘分析进行模式识别。结果:在造模后,模型组、电针组大鼠心电图均表现出T波高耸,R波与T波间无明显ST段。再灌注后T波下降超过0.2mV,无明显ST段。与正常组比较,模型组大鼠血清代谢模式发生明显改变,乙酰乙酸、乳酸、肌酸、丙三醇、葡萄糖升高,丙氨酸、谷氨酰胺、甘油磷酸胆碱、磷酸胆碱降低。与模型组比较,内关组大鼠血清代谢模式发生明显改变,表现为葡萄糖上升,亮氨酸、异亮氨酸、缬氨酸、三羟基丁酸、乳酸、醋酸盐、丙酮、乙酰乙酸、丙酮酸、谷氨酰胺、肌酸、丙三醇下降;合谷组与模型组代谢模式无显著差异。结论:电针"内关"预处理明显改变了MIRI大鼠糖代谢、丙酮酸代谢、氨基酸代谢、酮体代谢、能量代谢的模式,电针"内关"可能通过调节丙酮酸、氨基酸、酮体、能量等对MIRI大鼠起到一定的预保护作用。展开更多
目的:基于核磁共振氢谱(1 H NMR)研究电针"内关"穴对心肌缺血再灌注损伤(MIRI)大鼠血清和心肌组织代谢物的影响,探讨电针"内关"穴对MIRI大鼠代谢模式的影响。方法:30只SD雄性大鼠随机分为对照组、模型组、电针组。...目的:基于核磁共振氢谱(1 H NMR)研究电针"内关"穴对心肌缺血再灌注损伤(MIRI)大鼠血清和心肌组织代谢物的影响,探讨电针"内关"穴对MIRI大鼠代谢模式的影响。方法:30只SD雄性大鼠随机分为对照组、模型组、电针组。对照组:不予电针,仅用鼠板束缚大鼠,每次20min,每日1次,持续7d;模型组予心肌缺血再灌注造模:至第7天后结扎冠状动脉左前降支40 min后恢复血流60 min;电针组(频率10Hz/50Hz,脉冲宽度0.5ms,强度1mA):每日电针双侧"内关"穴1次,时间20min,持续7d,于第7天电针后造模。造模结束后,收集3组大鼠的血清和心肌组织。利用1 H NMR进行代谢物检测并利用多元统计方法主成分分析(PCA)、正交偏最小二乘分析(OPLS-DA)进行模式识别。结果:与对照组相比,模型组大鼠血清的亮氨酸、异亮氨酸、缬氨酸、3-羟基丁酸、乳酸、丙氨酸、赖氨酸、醋酸盐、N-乙酰糖蛋白、丙酮、乙酰乙酸、琥珀酸、谷氨酰胺、多不饱和脂肪酸、肌酸、甘油磷酸胆碱、甘氨酸均浓度下降,低密度脂蛋白/极低密度脂蛋白、葡萄糖浓度上升;其中除丙酮、乙酰乙酸和多不饱和脂肪酸外,低密度脂蛋白/极低密度脂蛋白在电针后下降到接近对照组水平,其它代谢物浓度均在电针后上升到接近对照组水平,电针组大鼠血清的代谢模式更接近对照组。与对照组相比,模型组大鼠心肌组织的乳酸、丙氨酸、赖氨酸、谷氨酸、谷氨酰胺、天冬氨酸、甘油磷酸胆碱、肌酸、牛磺酸、甘氨酸、苏氨酸、腺苷一磷酸、烟酰胺腺嘌呤二核苷酸浓度下降,葡萄糖浓度明显上升;其中肌酸、甘油磷酸胆碱、烟酰胺腺嘌呤二核苷酸浓度在电针后上升,葡萄糖浓度在电针后下降;此外,电针组大鼠心肌组织苏氨酸、腺苷一磷酸浓度较模型组进一步下降;电针组大鼠心肌的代谢模式虽然有向对照组偏移的趋势,但与对照组差异还是较大。�展开更多
Objective:To observe the effect of electroacupuncture(EA)pretreatment on adenine nucleotides in the myocardial tissues of the myocardial ischemia-reperfusion injury(MIRI)rats,and to explore the mechanism of EA pretrea...Objective:To observe the effect of electroacupuncture(EA)pretreatment on adenine nucleotides in the myocardial tissues of the myocardial ischemia-reperfusion injury(MIRI)rats,and to explore the mechanism of EA pretreatment on myocardial prevention and protection in MIRI rats.Methods:Forty SPF male Sprague-Dawley(SD)rats were randomly divided into 5 groups:a blank group,a sham operation group,a model group,an EA at Neiguan(PC 6)group and an EA at Hegu(LI 4)group,with 8 rats in each group.Rats in the blank group only received binding to the rat plate,30 min/time,once a day for 7 d;on the 7th day,rats in the sham operation group were subjected to threading for 40 min at the left anterior descending coronary artery without ligation,and then the rats were allowed to stand for 60 min before collection of the specimens;on the 7th day,rats in the model group were subjected to threading at the left anterior descending coronary artery with ligation,for 40 min before the blood flow was restored,and then the rats were allowed to stand for 60 min before collection of the specimens;on the 7th day of pretreatment with EA at Neiguan(PC 6)or Hegu(LI 4)for 30 min per day(once a day for 7 d),rats in the EA at Neiguan(PC 6)group and EA at Hegu(LI 4)group were subjected to modeling and sample collection same as in the model group.The left ventricular myocardium of the lower left anterior descending coronary artery was collected from rats in all 5 groups.Hematoxylin-eosin(HE)staining and transmission electron microscope(TEM)were used to observe the changes in myocardial pathological morphology.The change in the adenine nucleotide level of myocardial tissue was measured by high performance liquid chromatography(HPLC).Results:The HE staining and ultrastructure showed that the myocardial injury was severer in the model group compared with the sham operation group.Compared with the model group,the myocardial injury in the EA at Neiguan(PC 6)and the EA at Hegu(LI 4)groups was mild or hardly any.The adenine nucleotide levels in the sham ope展开更多
文摘目的:基于核磁共振氢谱技术(1 H NMR)观察电针"内关"穴预处理对心肌缺血再灌注损伤(MIRI)大鼠血清代谢物及其代谢通路的影响,从代谢组学角度探讨电针预处理对MIRI大鼠产生保护作用的机制。方法:雄性SD大鼠48只随机分为正常组、模型组、电针内关组(内关组)、电针合谷组(合谷组),每组12只。采用结扎冠状动脉左前降支40min后恢复血流1h制备MIRI大鼠模型。两电针组造模前每天予电针相应穴位30min,连续7d。模型制备成功后,收集各组大鼠血清,利用~1 H NMR进行代谢物检测,利用最小二乘分析、正交偏最小二乘分析进行模式识别。结果:在造模后,模型组、电针组大鼠心电图均表现出T波高耸,R波与T波间无明显ST段。再灌注后T波下降超过0.2mV,无明显ST段。与正常组比较,模型组大鼠血清代谢模式发生明显改变,乙酰乙酸、乳酸、肌酸、丙三醇、葡萄糖升高,丙氨酸、谷氨酰胺、甘油磷酸胆碱、磷酸胆碱降低。与模型组比较,内关组大鼠血清代谢模式发生明显改变,表现为葡萄糖上升,亮氨酸、异亮氨酸、缬氨酸、三羟基丁酸、乳酸、醋酸盐、丙酮、乙酰乙酸、丙酮酸、谷氨酰胺、肌酸、丙三醇下降;合谷组与模型组代谢模式无显著差异。结论:电针"内关"预处理明显改变了MIRI大鼠糖代谢、丙酮酸代谢、氨基酸代谢、酮体代谢、能量代谢的模式,电针"内关"可能通过调节丙酮酸、氨基酸、酮体、能量等对MIRI大鼠起到一定的预保护作用。
文摘目的:基于核磁共振氢谱(1 H NMR)研究电针"内关"穴对心肌缺血再灌注损伤(MIRI)大鼠血清和心肌组织代谢物的影响,探讨电针"内关"穴对MIRI大鼠代谢模式的影响。方法:30只SD雄性大鼠随机分为对照组、模型组、电针组。对照组:不予电针,仅用鼠板束缚大鼠,每次20min,每日1次,持续7d;模型组予心肌缺血再灌注造模:至第7天后结扎冠状动脉左前降支40 min后恢复血流60 min;电针组(频率10Hz/50Hz,脉冲宽度0.5ms,强度1mA):每日电针双侧"内关"穴1次,时间20min,持续7d,于第7天电针后造模。造模结束后,收集3组大鼠的血清和心肌组织。利用1 H NMR进行代谢物检测并利用多元统计方法主成分分析(PCA)、正交偏最小二乘分析(OPLS-DA)进行模式识别。结果:与对照组相比,模型组大鼠血清的亮氨酸、异亮氨酸、缬氨酸、3-羟基丁酸、乳酸、丙氨酸、赖氨酸、醋酸盐、N-乙酰糖蛋白、丙酮、乙酰乙酸、琥珀酸、谷氨酰胺、多不饱和脂肪酸、肌酸、甘油磷酸胆碱、甘氨酸均浓度下降,低密度脂蛋白/极低密度脂蛋白、葡萄糖浓度上升;其中除丙酮、乙酰乙酸和多不饱和脂肪酸外,低密度脂蛋白/极低密度脂蛋白在电针后下降到接近对照组水平,其它代谢物浓度均在电针后上升到接近对照组水平,电针组大鼠血清的代谢模式更接近对照组。与对照组相比,模型组大鼠心肌组织的乳酸、丙氨酸、赖氨酸、谷氨酸、谷氨酰胺、天冬氨酸、甘油磷酸胆碱、肌酸、牛磺酸、甘氨酸、苏氨酸、腺苷一磷酸、烟酰胺腺嘌呤二核苷酸浓度下降,葡萄糖浓度明显上升;其中肌酸、甘油磷酸胆碱、烟酰胺腺嘌呤二核苷酸浓度在电针后上升,葡萄糖浓度在电针后下降;此外,电针组大鼠心肌组织苏氨酸、腺苷一磷酸浓度较模型组进一步下降;电针组大鼠心肌的代谢模式虽然有向对照组偏移的趋势,但与对照组差异还是较大。�
文摘Objective:To observe the effect of electroacupuncture(EA)pretreatment on adenine nucleotides in the myocardial tissues of the myocardial ischemia-reperfusion injury(MIRI)rats,and to explore the mechanism of EA pretreatment on myocardial prevention and protection in MIRI rats.Methods:Forty SPF male Sprague-Dawley(SD)rats were randomly divided into 5 groups:a blank group,a sham operation group,a model group,an EA at Neiguan(PC 6)group and an EA at Hegu(LI 4)group,with 8 rats in each group.Rats in the blank group only received binding to the rat plate,30 min/time,once a day for 7 d;on the 7th day,rats in the sham operation group were subjected to threading for 40 min at the left anterior descending coronary artery without ligation,and then the rats were allowed to stand for 60 min before collection of the specimens;on the 7th day,rats in the model group were subjected to threading at the left anterior descending coronary artery with ligation,for 40 min before the blood flow was restored,and then the rats were allowed to stand for 60 min before collection of the specimens;on the 7th day of pretreatment with EA at Neiguan(PC 6)or Hegu(LI 4)for 30 min per day(once a day for 7 d),rats in the EA at Neiguan(PC 6)group and EA at Hegu(LI 4)group were subjected to modeling and sample collection same as in the model group.The left ventricular myocardium of the lower left anterior descending coronary artery was collected from rats in all 5 groups.Hematoxylin-eosin(HE)staining and transmission electron microscope(TEM)were used to observe the changes in myocardial pathological morphology.The change in the adenine nucleotide level of myocardial tissue was measured by high performance liquid chromatography(HPLC).Results:The HE staining and ultrastructure showed that the myocardial injury was severer in the model group compared with the sham operation group.Compared with the model group,the myocardial injury in the EA at Neiguan(PC 6)and the EA at Hegu(LI 4)groups was mild or hardly any.The adenine nucleotide levels in the sham ope
