Genetic diversity and genetic differentiation of 11 Manglietia yuyuanensis provenances from main distribution area were analyzed using ISSR molecular markers.A total of 108 amplified loci were detected by 9 primers,of...Genetic diversity and genetic differentiation of 11 Manglietia yuyuanensis provenances from main distribution area were analyzed using ISSR molecular markers.A total of 108 amplified loci were detected by 9 primers,of which the proportion of polymorphic loci was 86.11%.A high degree of genetic diversity in M.yuyuanensis was detected,and the genetic diversity index in species level was 0.282 7,which was markedly higher than that of M.decidua and M.patungensis,the rare and endangered species belonging to the same genus with M.yuyuanensis.The result showed that there was significant difference in genetic diversity among provenances.Provenances from north and north-west Fujian province and the nearby area,including Shaxian,Jian’ou,Wuyishan of Fujian and Longquan of Zhejiang,had higher genetic diversity,which were the dominant regions to be protected and utilized. Small population effect brought by habitat fragmentation and distance isolation effect resulted in high genetic differentiation among provenances,which contributed to 25.89% of total genetic variation,and the differentiation within provenances contributed to 74.11% of total genetic variation.Results also showed that the genetic distance amog provenances was not significantly related to the geographical distance amog provenances.Based on the Nei’s unbiased genetic distance,11 provenances might be obviously divided into 3 clusters.Shaxian and Jian’ou of Fujian with high genetic diversity and large nature population was clustered into one group.The genetic distance among Huangshan of Anhui,Longquan and Kaihua of Zhejiang,Wuyishan,Shaowu and Liancheng of Fujian,and Lichuan and Longnan of Jiangxi was small,then the above provenances were clustered.Quannan of Jiangxi,located at the south of distribution area,was an individual provenance.Based on the characteristic of its wider leaves,it was conferred that Quannan provenance was mistaken for M.fordiana Oliv.in the course of seed collection.展开更多
The provenance difference for photosynthetic characters of Taxus chinensis var.mairer seedling was studied by use of provenance trial under different shading condition located at Chun’an of Zhejiang.The result indica...The provenance difference for photosynthetic characters of Taxus chinensis var.mairer seedling was studied by use of provenance trial under different shading condition located at Chun’an of Zhejiang.The result indicated that there were obvious provenance differences for height growth,dry matter accumulation,chlorophyll a,b and the total content,photosynthesis rate with different geographical genetic variation pattern.Seedling height was found to be positively and significantly related to the altitude and longitude of its seed source.Contrasting to seedling height,dry matter appeared to be certainly and negatively related to the altitude and longitude of its seed source.There were no obvious geographical variation pattern for chlorophyll content and photosynthesis rate.The provenances with more chlorophyll had higher photosynthesis rate and greater dry matter accumulation.Analysis demonstrated that there existed great effect of light environment on photosynthetic character of seedling.At September,the chlorophyll a,b and total content of the seedlings under light transmittance 10% were 12.0%,16.1% and 13.7% more than the ones under light transmittance 25% respectively.At October,the chlorophyll a,b and total content of the most provenances were declined under light transmittance 10%,and average photosynthesis rate of the provenances tested was 13.7% less than the one under light transmittance 25%. The change of chlorophyll content of provenance seedling at various growth stages varied with light condition.Under light transmittance 25%,the content of chlorophyll was lower at August,highest at September,and decreased or increased slightly at October.Under light transmittance 10%,the content of chlorophyll at October was descended above 20% than one at September.展开更多
文摘Genetic diversity and genetic differentiation of 11 Manglietia yuyuanensis provenances from main distribution area were analyzed using ISSR molecular markers.A total of 108 amplified loci were detected by 9 primers,of which the proportion of polymorphic loci was 86.11%.A high degree of genetic diversity in M.yuyuanensis was detected,and the genetic diversity index in species level was 0.282 7,which was markedly higher than that of M.decidua and M.patungensis,the rare and endangered species belonging to the same genus with M.yuyuanensis.The result showed that there was significant difference in genetic diversity among provenances.Provenances from north and north-west Fujian province and the nearby area,including Shaxian,Jian’ou,Wuyishan of Fujian and Longquan of Zhejiang,had higher genetic diversity,which were the dominant regions to be protected and utilized. Small population effect brought by habitat fragmentation and distance isolation effect resulted in high genetic differentiation among provenances,which contributed to 25.89% of total genetic variation,and the differentiation within provenances contributed to 74.11% of total genetic variation.Results also showed that the genetic distance amog provenances was not significantly related to the geographical distance amog provenances.Based on the Nei’s unbiased genetic distance,11 provenances might be obviously divided into 3 clusters.Shaxian and Jian’ou of Fujian with high genetic diversity and large nature population was clustered into one group.The genetic distance among Huangshan of Anhui,Longquan and Kaihua of Zhejiang,Wuyishan,Shaowu and Liancheng of Fujian,and Lichuan and Longnan of Jiangxi was small,then the above provenances were clustered.Quannan of Jiangxi,located at the south of distribution area,was an individual provenance.Based on the characteristic of its wider leaves,it was conferred that Quannan provenance was mistaken for M.fordiana Oliv.in the course of seed collection.
文摘The provenance difference for photosynthetic characters of Taxus chinensis var.mairer seedling was studied by use of provenance trial under different shading condition located at Chun’an of Zhejiang.The result indicated that there were obvious provenance differences for height growth,dry matter accumulation,chlorophyll a,b and the total content,photosynthesis rate with different geographical genetic variation pattern.Seedling height was found to be positively and significantly related to the altitude and longitude of its seed source.Contrasting to seedling height,dry matter appeared to be certainly and negatively related to the altitude and longitude of its seed source.There were no obvious geographical variation pattern for chlorophyll content and photosynthesis rate.The provenances with more chlorophyll had higher photosynthesis rate and greater dry matter accumulation.Analysis demonstrated that there existed great effect of light environment on photosynthetic character of seedling.At September,the chlorophyll a,b and total content of the seedlings under light transmittance 10% were 12.0%,16.1% and 13.7% more than the ones under light transmittance 25% respectively.At October,the chlorophyll a,b and total content of the most provenances were declined under light transmittance 10%,and average photosynthesis rate of the provenances tested was 13.7% less than the one under light transmittance 25%. The change of chlorophyll content of provenance seedling at various growth stages varied with light condition.Under light transmittance 25%,the content of chlorophyll was lower at August,highest at September,and decreased or increased slightly at October.Under light transmittance 10%,the content of chlorophyll at October was descended above 20% than one at September.
