Fungi blast is one of the most serious diseases of rice worldwide. Breeding resistant varieties have been proved to be the most effective and economical means to control the disease. This paper describes the molecular...Fungi blast is one of the most serious diseases of rice worldwide. Breeding resistant varieties have been proved to be the most effective and economical means to control the disease. This paper describes the molecular marker-assisted selection (MAS) procedure for a broad-spectrum blast resistant gene Pi1 integrated into an elite hybrid maintainer line, Zhenshan 97. A simple sequence repeat (SSR) based on molecular marker-aided selection system for Pi1 segment was established. Using a backcross population and a blast isolate F1829, Pi1 gene was mapped on the top of chromosome 11 between markers RZ536 and RM144, with a distance of 9.7 cM and 6.8 cM, respectively. Seventeen families derived from the recurrent parent Zhenshan 97 were obtained with homozygous Pi1 gene. The background of the 17 families was identified with inter simple sequence repeat (ISSR) amplification, the highest recovery of the Zhenshan 97 genetic background was 97.01% after the assay of 167 polymorphic bands.展开更多
文章以Web of Science TM核心集中图书情报学科的"大数据""数据驱动"文献为数据源,分析大数据驱动下图书情报学科研究的现状和进展;借助SATI和SPSS软件对507篇文献的关键词进行共词分析和聚类分析。研究表明:大数...文章以Web of Science TM核心集中图书情报学科的"大数据""数据驱动"文献为数据源,分析大数据驱动下图书情报学科研究的现状和进展;借助SATI和SPSS软件对507篇文献的关键词进行共词分析和聚类分析。研究表明:大数据驱动下的图书情报学科研究热点主题主要集中在数字图书馆知识组织与语义互联、社会网络大数据、科研大数据管理与共享、云计算与信息安全、政府数据开放与共享、大数据驱动的知识发现、E-learning与高等教育、数据挖掘与数字人文等方面。展开更多
文摘Fungi blast is one of the most serious diseases of rice worldwide. Breeding resistant varieties have been proved to be the most effective and economical means to control the disease. This paper describes the molecular marker-assisted selection (MAS) procedure for a broad-spectrum blast resistant gene Pi1 integrated into an elite hybrid maintainer line, Zhenshan 97. A simple sequence repeat (SSR) based on molecular marker-aided selection system for Pi1 segment was established. Using a backcross population and a blast isolate F1829, Pi1 gene was mapped on the top of chromosome 11 between markers RZ536 and RM144, with a distance of 9.7 cM and 6.8 cM, respectively. Seventeen families derived from the recurrent parent Zhenshan 97 were obtained with homozygous Pi1 gene. The background of the 17 families was identified with inter simple sequence repeat (ISSR) amplification, the highest recovery of the Zhenshan 97 genetic background was 97.01% after the assay of 167 polymorphic bands.
文摘文章以Web of Science TM核心集中图书情报学科的"大数据""数据驱动"文献为数据源,分析大数据驱动下图书情报学科研究的现状和进展;借助SATI和SPSS软件对507篇文献的关键词进行共词分析和聚类分析。研究表明:大数据驱动下的图书情报学科研究热点主题主要集中在数字图书馆知识组织与语义互联、社会网络大数据、科研大数据管理与共享、云计算与信息安全、政府数据开放与共享、大数据驱动的知识发现、E-learning与高等教育、数据挖掘与数字人文等方面。
