The effects of combined use of earthworm extract（912）and HpD-laser on the produc-tion of reactive oxygen and the biosynthesis of DNA in S<sub>180</sub> tumor cells were studied throughchemiluminescence m...The effects of combined use of earthworm extract（912）and HpD-laser on the produc-tion of reactive oxygen and the biosynthesis of DNA in S<sub>180</sub> tumor cells were studied throughchemiluminescence measurement and[<sup>3</sup>H]-TdR incorporation assay.The results showed that as com-pared with the control,the intensity of chemiluminescence emitted by tumor cells treatedsimultaneously with 912 and HpD-laser was enhanced more than ten-folds,while that treated with912 or HpD-laser alone was increased only 2～4 folds.The[<sup>3</sup>H]-TdR incorporation into tumorcells of the former group was inhibited upto 74.1%,and that of the latter groups decreased onlyby 42.2% and 40.0%,respectively.In accordance with these biochemical changes,the ultrastructuraldamage of tumor cells of the former,combinedly treated group appeared to be the most serious.This suggests an additive effect of 912 with HpD-laser on tumor cells.In addition,if free radicalscavengers,such as catalase and superoxide dismutase,were added to the reaction systembefore chemiluminescence assay,the luminescent enhancement effect mentioned above was dramaticallyalleviated,implying the presence of O<sub>2</sub><sup> </sup>and H<sub>2</sub>O<sub>2</sub> in the system.Therefore,as to the toxic effecton tumor cells,912 and HpD-laser are not only additive in efficiency,but also similar in theunderlying mechanism of action.展开更多
In this study, we found, through biochemical synthesis of DNA with the incorporation of ~3HTdR and morphological observation of the tumor cells, that aidiron (912), an extract from earthworm, had a stronger kilting ef...In this study, we found, through biochemical synthesis of DNA with the incorporation of ~3HTdR and morphological observation of the tumor cells, that aidiron (912), an extract from earthworm, had a stronger kilting effect (27%) on mouse ascitic hepatoma tumor cells (H_(22)) in vitro, and that laser-hematoporphyrin derivative (HpD) combined with 912 markedly enhanced the kilting effect (74%) on tumor cells. By means of the fluoresoence spectrophotography we also dis- covered that the killing effect of laser-HpD-912 on tumor calls was closely related with the produc- tion of free radicals and lipid peroxidation.展开更多
This study examines the effects of x-adrenergic stimulation on coronaryvasodilation induced by selective doses of intracoronary adenosine.In anesthetized open-chest dogs,the left anterior descending coronary artery（...This study examines the effects of x-adrenergic stimulation on coronaryvasodilation induced by selective doses of intracoronary adenosine.In anesthetized open-chest dogs,the left anterior descending coronary artery（LAD）was perfused at a constant flow and its perfusionpressure was measured,pr ctolol（2.5 mg）was infused into the LAD to block β<sub>1</sub>-adrenergicreceptors in the myocardium.Intracoronary adenosine at increasing doses produced graded decreasesin coronary vascular resistance by 8.7%（2μg/min）,20.6%（10/μg/min）and 28%（50 μg/min）of the control.Identical doses（6μg）of intracoronary norepinephfine produced increases in coronaryvascular resistance by 17.4%（adenosine 2 μg/min）,23.2%（adenosine 10μg/min）and 34.1%（adenosine 50 μg/min）of the control when coronary vessels were dilated by increasing doses ofadenosine,respectively.It is suggested that the constrictive effect of x-adrenergic stimulalion can ex-ert a limiting effect on adenosine-induced coronary vasodilation.展开更多
基金Supported by the National Natural Science Foundation of China No.3860835
文摘The effects of combined use of earthworm extract（912）and HpD-laser on the produc-tion of reactive oxygen and the biosynthesis of DNA in S<sub>180</sub> tumor cells were studied throughchemiluminescence measurement and[<sup>3</sup>H]-TdR incorporation assay.The results showed that as com-pared with the control,the intensity of chemiluminescence emitted by tumor cells treatedsimultaneously with 912 and HpD-laser was enhanced more than ten-folds,while that treated with912 or HpD-laser alone was increased only 2～4 folds.The[<sup>3</sup>H]-TdR incorporation into tumorcells of the former group was inhibited upto 74.1%,and that of the latter groups decreased onlyby 42.2% and 40.0%,respectively.In accordance with these biochemical changes,the ultrastructuraldamage of tumor cells of the former,combinedly treated group appeared to be the most serious.This suggests an additive effect of 912 with HpD-laser on tumor cells.In addition,if free radicalscavengers,such as catalase and superoxide dismutase,were added to the reaction systembefore chemiluminescence assay,the luminescent enhancement effect mentioned above was dramaticallyalleviated,implying the presence of O<sub>2</sub><sup> </sup>and H<sub>2</sub>O<sub>2</sub> in the system.Therefore,as to the toxic effecton tumor cells,912 and HpD-laser are not only additive in efficiency,but also similar in theunderlying mechanism of action.
文摘In this study, we found, through biochemical synthesis of DNA with the incorporation of ~3HTdR and morphological observation of the tumor cells, that aidiron (912), an extract from earthworm, had a stronger kilting effect (27%) on mouse ascitic hepatoma tumor cells (H_(22)) in vitro, and that laser-hematoporphyrin derivative (HpD) combined with 912 markedly enhanced the kilting effect (74%) on tumor cells. By means of the fluoresoence spectrophotography we also dis- covered that the killing effect of laser-HpD-912 on tumor calls was closely related with the produc- tion of free radicals and lipid peroxidation.
文摘This study examines the effects of x-adrenergic stimulation on coronaryvasodilation induced by selective doses of intracoronary adenosine.In anesthetized open-chest dogs,the left anterior descending coronary artery（LAD）was perfused at a constant flow and its perfusionpressure was measured,pr ctolol（2.5 mg）was infused into the LAD to block β<sub>1</sub>-adrenergicreceptors in the myocardium.Intracoronary adenosine at increasing doses produced graded decreasesin coronary vascular resistance by 8.7%（2μg/min）,20.6%（10/μg/min）and 28%（50 μg/min）of the control.Identical doses（6μg）of intracoronary norepinephfine produced increases in coronaryvascular resistance by 17.4%（adenosine 2 μg/min）,23.2%（adenosine 10μg/min）and 34.1%（adenosine 50 μg/min）of the control when coronary vessels were dilated by increasing doses ofadenosine,respectively.It is suggested that the constrictive effect of x-adrenergic stimulalion can ex-ert a limiting effect on adenosine-induced coronary vasodilation.
