The vertical slab polyacrylamide gel electrophoretic technique is used to examine the esterase isozyme of four species (Hemiptera: Coreidae), the analysis has revealed that esterase zymogram can be divided into four z...The vertical slab polyacrylamide gel electrophoretic technique is used to examine the esterase isozyme of four species (Hemiptera: Coreidae), the analysis has revealed that esterase zymogram can be divided into four zones. Esterase zymogram of intergeneric species show distinct difference, while some intrageneric common features are discovered. The clustering analysis shows that the taxonomic status of 4 species, and sex variance is bigger than individuals and is smaller than species.展开更多
[ Objective ] The paper was to explore the phylogenetic relationship among 10 species of insects in Cletus, Hygia, Acanthocoris, Coreus and Homoeo- cerus in Coreinae. [ Method] Using vertical slab PAGE, EST isozymes o...[ Objective ] The paper was to explore the phylogenetic relationship among 10 species of insects in Cletus, Hygia, Acanthocoris, Coreus and Homoeo- cerus in Coreinae. [ Method] Using vertical slab PAGE, EST isozymes of 10 species of Riptortus linearis in Coreinae were studied. [ Result] Cluster analysis showed that the difference between 5 genera of Coreinae were greater than intraspecific differences. The genus Homoeocerus had more distant relationship with the other 4 genera of Coreinae, while it had cleser relationship with Mictinae. [ Conclusion] EST isozymes of 10 species of the subfamily were clear with strong pely- morphic and good stability, which could be used for insect identification of Coreinae and phylogenetic study of species. Combined with morphological characters and variation condition of Cyt b gene sequence, it is suggested that Homoeocerus should be separated from Coreinae and classified into Mictinae.展开更多
文摘The vertical slab polyacrylamide gel electrophoretic technique is used to examine the esterase isozyme of four species (Hemiptera: Coreidae), the analysis has revealed that esterase zymogram can be divided into four zones. Esterase zymogram of intergeneric species show distinct difference, while some intrageneric common features are discovered. The clustering analysis shows that the taxonomic status of 4 species, and sex variance is bigger than individuals and is smaller than species.
文摘[ Objective ] The paper was to explore the phylogenetic relationship among 10 species of insects in Cletus, Hygia, Acanthocoris, Coreus and Homoeo- cerus in Coreinae. [ Method] Using vertical slab PAGE, EST isozymes of 10 species of Riptortus linearis in Coreinae were studied. [ Result] Cluster analysis showed that the difference between 5 genera of Coreinae were greater than intraspecific differences. The genus Homoeocerus had more distant relationship with the other 4 genera of Coreinae, while it had cleser relationship with Mictinae. [ Conclusion] EST isozymes of 10 species of the subfamily were clear with strong pely- morphic and good stability, which could be used for insect identification of Coreinae and phylogenetic study of species. Combined with morphological characters and variation condition of Cyt b gene sequence, it is suggested that Homoeocerus should be separated from Coreinae and classified into Mictinae.
