Background Chemokine-like factor 1 (CKLF1) was recently identified as a novel cytokine The full-length CKLF1 cDNA contains 530 bp encoding 99 amino acid residues with a CC motif similar to that of other CC family c...Background Chemokine-like factor 1 (CKLF1) was recently identified as a novel cytokine The full-length CKLF1 cDNA contains 530 bp encoding 99 amino acid residues with a CC motif similar to that of other CC family chemokines Recombinant CKLF1 exhibits chemotactic activity on leucocytes and stimulates proliferation of murine skeletal muscle cells We questioned whether CKLF1 could be involved in the pathogenesis of inflammation and proliferation in the lung Therefore we used efficient in vivo gene delivery method to investigate the biological effect of CKLF1 in the murine lung Methods CKLF1-expressing plasmid, pCDI-CKLF1, was constructed and injected into the skeletal muscles followed by electroporation Lung tissues were obtained at the end of week 1,2,3 and 4 respectively after injection The pathological changes in the lungs were observed by light microscope Results A single intramuscular injection of CKLF1 plasmid DNA into BALB/c mice caused dramatic pathological changes in the lungs of treated mice These changes included peribronchial leukocyte infiltration, epithelial shedding, collagen deposition, proliferation of bronchial smooth muscle cells and fibrosis of the lung Conclusions The sustained morphological abnormalities of the bronchial and bronchiolar wall, the acute pneumonitis and interstitial pulmonary fibrosis induced by CKLF1 were similar to phenomena observed in chronic persistent asthma, acute respiratory distress syndrome and severe acute respiratory syndrome These data suggest that CKLF1 may play an important role in the pathogenesis of these important diseases and the study also implies that gene electro-transfer in vivo could serve as a valuable approach for evaluating the function of a novel gene in animals展开更多
Background Immunostimulating agents made from bacterial extracts represent a class of medications that contains antigens derived from several bacterial strains and their potential ability to prevent bacterial infectio...Background Immunostimulating agents made from bacterial extracts represent a class of medications that contains antigens derived from several bacterial strains and their potential ability to prevent bacterial infections results from the stimulation of the nonspecific component of the immune system. The present study investigated the effect of the oral immunostimulant Broncho-Vaxom,which includes material from eight different species of bacteria that are frequently present in the lower respiratory tract,on the frequency and severity of acute exacerbation in patients with chronic bronchitis accompanied by chronic obstructive pulmonary disease (COPD). Methods Ninety patients with chronic bronchitis complicated with COPD were randomly divided into groups A and B. Forty-nine subjects in group A received oral capsules containing 7mg Broncho-Vaxom,while 41 patients in group B received similar placebo capsules. Both groups took one capsule daily for the first 10 days of each month for 3 consecutive months. The frequency of acute exacerbation,symptom scores,and lung function were recorded for the following one year period.Results There was a significant decrease in the incidence,duration,and severity of acute exacerbation,as well as a reduction in the course of antibiotics administered and in the dosage of bronchodilator and mucolytic agent in group A,as compared to group B ( P <0.05,respectively). Symptom scores for cough,sputum,dyspnea,as well as symptoms observed upon auscultation of the chest also improved significantly in group A as compared to group B ( P <0.05,respectively). The bacterial clearance rate in sputum cultures from patients who received no antibiotics for the first 3 months was also significantly higher in group A compared to group B ( P <0.01).Conclusions Orally administered Broncho-Vaxom is associated with a decrease in the incidence of acute exacerbation and a decrease in the need for antibiotics and symptomatic relief medications in patients with chronic bronchitis accompanied by COPD. Broncho-Vaxom展开更多
【目的】分析和讨论国外学术期刊中近期开展的小组同行评议与协作同行评议的概念、流程以及优缺点,为国内期刊同行评议制度的改革提供借鉴与参考。【方法】检索Web of Science、Pub Med数据库与网络信息,筛选、整理这两种同行评议模式...【目的】分析和讨论国外学术期刊中近期开展的小组同行评议与协作同行评议的概念、流程以及优缺点,为国内期刊同行评议制度的改革提供借鉴与参考。【方法】检索Web of Science、Pub Med数据库与网络信息,筛选、整理这两种同行评议模式的相关文献与资料,采用文献综述法进行主题归纳与分析。【结果】这两种同行评议模式都可能对改善审稿人表现、提高审稿报告质量以及审稿过程的透明度有一定的作用,但也存在受集体思考的影响、时间投入增加等局限性。【结论】小组同行评议与协作同行评议模式对我国期刊同行评议的发展和创新有可借鉴之处,国内期刊可在开展集体对话、构建更具互动性的审稿机制、开展线下小组评审等方面作出更多尝试。展开更多
Objective To determine the efficacy and incidence of complications of extracorporeal shock wave lithotripsy (ESWL) compared with pneumatic ureteroscopic lithotripsy (URSL) in the treatment of lower uretheral calculi...Objective To determine the efficacy and incidence of complications of extracorporeal shock wave lithotripsy (ESWL) compared with pneumatic ureteroscopic lithotripsy (URSL) in the treatment of lower uretheral calculi Methods From August 1997 to June 1999, 210 patients with lower ureteric calculi were treated with ESWL and the other 180 with URSL The stones were fragmented with pneumatic lithotripter The outcome was assessed by evacuation, retreatment and complication rates Results ESWL for lower ureteric calculi resulted in a stone evacuation rate of 78 1%, compared with 93 3% for URSL ( P <0 05) ESWL had a retreatment rate of 11 9% and a perforation rate of 0, while URSL caused perforation of ureters in 3 3% of patients and a refreatment of 2.2% Conclusion For the management of lower ureteric calculi, ESWL provides a non invasive, simple and safe option, and URSL has a higher stone evacuation rate but causes ureter perforation more frequently than ESWL does Both ESWL and URSL have their respective advantages It is recommended, however, that URSL be extensively developed for better treatment efficacy, given that the operator has an adequate technical background展开更多
目的:本文以呼吸医学为切入点,分析呼吸医学高引文作者群体及其选刊的10年对比,分析相对高影响力研究等作者选刊所看重的期刊特征。方法:检索科睿唯安Web of Science数据库中,呼吸系统高被引论文(Highly cited papers)。使用Citespace工...目的:本文以呼吸医学为切入点,分析呼吸医学高引文作者群体及其选刊的10年对比,分析相对高影响力研究等作者选刊所看重的期刊特征。方法:检索科睿唯安Web of Science数据库中,呼吸系统高被引论文(Highly cited papers)。使用Citespace工具,对过去10年高被引文的趋势进行分析。结果:亚洲及南美国家的高引文数量均有明显增加。《柳叶刀·呼吸医学》的影响力逐渐增强。创刊10年,能快速崛起的原因,可能在于《柳叶刀》的品牌化效应,及其高质量的审稿等因素。结论:注重期刊的品牌建设,提高审稿效率和质量,能增加医学期刊对高质量研究的吸引力。展开更多
基金ThisworkwassupportedbythegrantsfromtheGuangdongNaturalScienceFoundationKeyProgram (No 0 20741),theNationalNaturalScienceFoundationofChina (No 3 0 3 70 62 2),theNationalHighTechnologyResearchandDevelopmentProgram (10 2 0 8070499),andtheSARSResearchFoundat
文摘Background Chemokine-like factor 1 (CKLF1) was recently identified as a novel cytokine The full-length CKLF1 cDNA contains 530 bp encoding 99 amino acid residues with a CC motif similar to that of other CC family chemokines Recombinant CKLF1 exhibits chemotactic activity on leucocytes and stimulates proliferation of murine skeletal muscle cells We questioned whether CKLF1 could be involved in the pathogenesis of inflammation and proliferation in the lung Therefore we used efficient in vivo gene delivery method to investigate the biological effect of CKLF1 in the murine lung Methods CKLF1-expressing plasmid, pCDI-CKLF1, was constructed and injected into the skeletal muscles followed by electroporation Lung tissues were obtained at the end of week 1,2,3 and 4 respectively after injection The pathological changes in the lungs were observed by light microscope Results A single intramuscular injection of CKLF1 plasmid DNA into BALB/c mice caused dramatic pathological changes in the lungs of treated mice These changes included peribronchial leukocyte infiltration, epithelial shedding, collagen deposition, proliferation of bronchial smooth muscle cells and fibrosis of the lung Conclusions The sustained morphological abnormalities of the bronchial and bronchiolar wall, the acute pneumonitis and interstitial pulmonary fibrosis induced by CKLF1 were similar to phenomena observed in chronic persistent asthma, acute respiratory distress syndrome and severe acute respiratory syndrome These data suggest that CKLF1 may play an important role in the pathogenesis of these important diseases and the study also implies that gene electro-transfer in vivo could serve as a valuable approach for evaluating the function of a novel gene in animals
文摘Background Immunostimulating agents made from bacterial extracts represent a class of medications that contains antigens derived from several bacterial strains and their potential ability to prevent bacterial infections results from the stimulation of the nonspecific component of the immune system. The present study investigated the effect of the oral immunostimulant Broncho-Vaxom,which includes material from eight different species of bacteria that are frequently present in the lower respiratory tract,on the frequency and severity of acute exacerbation in patients with chronic bronchitis accompanied by chronic obstructive pulmonary disease (COPD). Methods Ninety patients with chronic bronchitis complicated with COPD were randomly divided into groups A and B. Forty-nine subjects in group A received oral capsules containing 7mg Broncho-Vaxom,while 41 patients in group B received similar placebo capsules. Both groups took one capsule daily for the first 10 days of each month for 3 consecutive months. The frequency of acute exacerbation,symptom scores,and lung function were recorded for the following one year period.Results There was a significant decrease in the incidence,duration,and severity of acute exacerbation,as well as a reduction in the course of antibiotics administered and in the dosage of bronchodilator and mucolytic agent in group A,as compared to group B ( P <0.05,respectively). Symptom scores for cough,sputum,dyspnea,as well as symptoms observed upon auscultation of the chest also improved significantly in group A as compared to group B ( P <0.05,respectively). The bacterial clearance rate in sputum cultures from patients who received no antibiotics for the first 3 months was also significantly higher in group A compared to group B ( P <0.01).Conclusions Orally administered Broncho-Vaxom is associated with a decrease in the incidence of acute exacerbation and a decrease in the need for antibiotics and symptomatic relief medications in patients with chronic bronchitis accompanied by COPD. Broncho-Vaxom
文摘【目的】分析和讨论国外学术期刊中近期开展的小组同行评议与协作同行评议的概念、流程以及优缺点,为国内期刊同行评议制度的改革提供借鉴与参考。【方法】检索Web of Science、Pub Med数据库与网络信息,筛选、整理这两种同行评议模式的相关文献与资料,采用文献综述法进行主题归纳与分析。【结果】这两种同行评议模式都可能对改善审稿人表现、提高审稿报告质量以及审稿过程的透明度有一定的作用,但也存在受集体思考的影响、时间投入增加等局限性。【结论】小组同行评议与协作同行评议模式对我国期刊同行评议的发展和创新有可借鉴之处,国内期刊可在开展集体对话、构建更具互动性的审稿机制、开展线下小组评审等方面作出更多尝试。
文摘Objective To determine the efficacy and incidence of complications of extracorporeal shock wave lithotripsy (ESWL) compared with pneumatic ureteroscopic lithotripsy (URSL) in the treatment of lower uretheral calculi Methods From August 1997 to June 1999, 210 patients with lower ureteric calculi were treated with ESWL and the other 180 with URSL The stones were fragmented with pneumatic lithotripter The outcome was assessed by evacuation, retreatment and complication rates Results ESWL for lower ureteric calculi resulted in a stone evacuation rate of 78 1%, compared with 93 3% for URSL ( P <0 05) ESWL had a retreatment rate of 11 9% and a perforation rate of 0, while URSL caused perforation of ureters in 3 3% of patients and a refreatment of 2.2% Conclusion For the management of lower ureteric calculi, ESWL provides a non invasive, simple and safe option, and URSL has a higher stone evacuation rate but causes ureter perforation more frequently than ESWL does Both ESWL and URSL have their respective advantages It is recommended, however, that URSL be extensively developed for better treatment efficacy, given that the operator has an adequate technical background
文摘目的:本文以呼吸医学为切入点,分析呼吸医学高引文作者群体及其选刊的10年对比,分析相对高影响力研究等作者选刊所看重的期刊特征。方法:检索科睿唯安Web of Science数据库中,呼吸系统高被引论文(Highly cited papers)。使用Citespace工具,对过去10年高被引文的趋势进行分析。结果:亚洲及南美国家的高引文数量均有明显增加。《柳叶刀·呼吸医学》的影响力逐渐增强。创刊10年,能快速崛起的原因,可能在于《柳叶刀》的品牌化效应,及其高质量的审稿等因素。结论:注重期刊的品牌建设,提高审稿效率和质量,能增加医学期刊对高质量研究的吸引力。
