β-glucuronidase (GUS) gene (carried by pBI121 plasmid) has been introduced into hexaploid wheat c. v. Xiaoshan No. 3 with the pollen-tube pathway method. Five transgenic plants are selected from 106 T0 plants. All of...β-glucuronidase (GUS) gene (carried by pBI121 plasmid) has been introduced into hexaploid wheat c. v. Xiaoshan No. 3 with the pollen-tube pathway method. Five transgenic plants are selected from 106 T0 plants. All of these plants are identified by the Southern hybridization, and the GUS activity can be detected by using fluorogenic assay and histochemical stain.展开更多
Ⅰ. INTRODUCTIONIn pollen mother cell (PMC) of wheat pollen-derived haploid, there are only 21 chromosomes (n=21), each of which lacks its homologous partner, thus the meiosis is abnormal and results in the production...Ⅰ. INTRODUCTIONIn pollen mother cell (PMC) of wheat pollen-derived haploid, there are only 21 chromosomes (n=21), each of which lacks its homologous partner, thus the meiosis is abnormal and results in the production of the microspores varying in size. Among them, some very small microspores which contain only a few chromosomes packed in a micro-展开更多
文摘β-glucuronidase (GUS) gene (carried by pBI121 plasmid) has been introduced into hexaploid wheat c. v. Xiaoshan No. 3 with the pollen-tube pathway method. Five transgenic plants are selected from 106 T0 plants. All of these plants are identified by the Southern hybridization, and the GUS activity can be detected by using fluorogenic assay and histochemical stain.
文摘Ⅰ. INTRODUCTIONIn pollen mother cell (PMC) of wheat pollen-derived haploid, there are only 21 chromosomes (n=21), each of which lacks its homologous partner, thus the meiosis is abnormal and results in the production of the microspores varying in size. Among them, some very small microspores which contain only a few chromosomes packed in a micro-
