MAPK (mitogen-activated protein kinase)信号级联系统在调节植物生长发育和逆境胁迫中具有重要作用。以不同抗感纹枯病水平的水稻品种为材料,分析OsMPKs类基因对水稻纹枯病菌的侵染响应特征,发现供检测的17个OsMPKs基因对纹枯病菌处...MAPK (mitogen-activated protein kinase)信号级联系统在调节植物生长发育和逆境胁迫中具有重要作用。以不同抗感纹枯病水平的水稻品种为材料,分析OsMPKs类基因对水稻纹枯病菌的侵染响应特征,发现供检测的17个OsMPKs基因对纹枯病菌处理后均有不同程度的表达响应,且在抗病、感病品种间存在一定差异。通过茉莉酸、水杨酸和乙烯处理,发现OsMPKs基因至少受到1种激素的诱导表达,且对不同激素的处理响应存在差异。组织表达分析显示,OsMPK3、OsMPK6等6个OsMPKs基因在纹枯病菌主要侵染部位叶鞘和叶片中有较强表达。综合比较不同表达分析结果,发现一些可能参与调控水稻对纹枯病抗性的OsMPKs基因。展开更多
A differential excitation probe based on eddy current testing technology was designed. Sheet specimens of Q 235 steel with prefabricated micro-cracks of different widths and of aluminum with prefabricated micro-cracks...A differential excitation probe based on eddy current testing technology was designed. Sheet specimens of Q 235 steel with prefabricated micro-cracks of different widths and of aluminum with prefabricated micro-cracks of different depths were detected through the designed detection system. The characteristics of micro-cracks can be clearly showed after signals processing through the short-time Fourier transform（ STFT）. By changing the parameter and its value in detecting process,the factors including the excitation frequency and amplitude,the lift-off effect and the scanning direction were discussed,respectively. The results showed that the differential excitation probe was insensitive to dimension and surface state of the tested specimen,while it had a high degree of recognition for micro-crack detection. Therefore,when the differential excitation detection technology was used for inspecting micro-crack of turbine blade in aero-engine,and smoothed pseudo Wigner-Ville distribution was used for signal processing,micro-cracks of 0. 3 mm depth and 0. 1 mm width could be identified. The experimental results might be useful for further research on engineering test of turbine blades of aero-engine.展开更多
文摘MAPK (mitogen-activated protein kinase)信号级联系统在调节植物生长发育和逆境胁迫中具有重要作用。以不同抗感纹枯病水平的水稻品种为材料,分析OsMPKs类基因对水稻纹枯病菌的侵染响应特征,发现供检测的17个OsMPKs基因对纹枯病菌处理后均有不同程度的表达响应,且在抗病、感病品种间存在一定差异。通过茉莉酸、水杨酸和乙烯处理,发现OsMPKs基因至少受到1种激素的诱导表达,且对不同激素的处理响应存在差异。组织表达分析显示,OsMPK3、OsMPK6等6个OsMPKs基因在纹枯病菌主要侵染部位叶鞘和叶片中有较强表达。综合比较不同表达分析结果,发现一些可能参与调控水稻对纹枯病抗性的OsMPKs基因。
基金Supported by the Ministerial Level Advanced Research Foundation(051317030586)Ph.D.Programs Foundation of the Ministry of Education of China(20121101110018)
文摘A differential excitation probe based on eddy current testing technology was designed. Sheet specimens of Q 235 steel with prefabricated micro-cracks of different widths and of aluminum with prefabricated micro-cracks of different depths were detected through the designed detection system. The characteristics of micro-cracks can be clearly showed after signals processing through the short-time Fourier transform（ STFT）. By changing the parameter and its value in detecting process,the factors including the excitation frequency and amplitude,the lift-off effect and the scanning direction were discussed,respectively. The results showed that the differential excitation probe was insensitive to dimension and surface state of the tested specimen,while it had a high degree of recognition for micro-crack detection. Therefore,when the differential excitation detection technology was used for inspecting micro-crack of turbine blade in aero-engine,and smoothed pseudo Wigner-Ville distribution was used for signal processing,micro-cracks of 0. 3 mm depth and 0. 1 mm width could be identified. The experimental results might be useful for further research on engineering test of turbine blades of aero-engine.
