The chromosome scaffolds in higher eukaryotic nuclei have been described elsewhere. Butit is unknown when they evolved. The dinoflagellates are the primitive organisms that maybe the intermediate between prokaryotes a...The chromosome scaffolds in higher eukaryotic nuclei have been described elsewhere. Butit is unknown when they evolved. The dinoflagellates are the primitive organisms that maybe the intermediate between prokaryotes and eukaryotes. Combining chromosome scaffold prep-aration methods with embedment-free section microscopy,we demonstrate that the dino-flagellate Crypthecodinium cohnii chromosome retains a protein scaffold after the depletionof DNA and soluble proteins. This scaffold preserves the morphology characteristic of thechromosome. Two-dimensional electrophoreses show that the chromosome scaffolds are mainlycomposed of acidic proteins. Our results suggest that a framework similar to the chromosomescaffold in the mammalian cell appeared in the primitive eukaryote. We propose that thechromosome scaffold possibly originated from the early stages of eukaryote evolution.展开更多
The nuclear lamina is a meshwork of intermediate filament-like fibrils situated immediately subjacent to the inner membrane of the nuclear envelope. Together with other proteinaceous elements such as the nuclear pore ...The nuclear lamina is a meshwork of intermediate filament-like fibrils situated immediately subjacent to the inner membrane of the nuclear envelope. Together with other proteinaceous elements such as the nuclear pore complex, the lamina forms part of the karyoskeleton, or structural framework of the nucleus. The lamina is thought to play a role in anchoring the nuclear envelope, and/or providing attachment sites for interphase chromatin. Mammalian somatic cells commonly contain three major lamins: A, B and C.展开更多
Isolation and characteriation of functional nudear matrix proteins involved in DNA anchoring and gene expression is one of the major subjects of current nudear matrix research. Southwestern blotting (DNA-protein hybri...Isolation and characteriation of functional nudear matrix proteins involved in DNA anchoring and gene expression is one of the major subjects of current nudear matrix research. Southwestern blotting (DNA-protein hybridization) was applied to studying the anchoring of c-myc on the nudear matrix proteins in mouse thymic T lymphocytes. The results showed that c-myc bound to the lamin, p34 and p36 nudear matrix proteins specifically. In the process of mouse thymic PNA T lymphocytes proliferation induced by ConA, the anchoring of c-myc on p34 and p36 nudear matrix proteins changed dynamically.展开更多
文摘The chromosome scaffolds in higher eukaryotic nuclei have been described elsewhere. Butit is unknown when they evolved. The dinoflagellates are the primitive organisms that maybe the intermediate between prokaryotes and eukaryotes. Combining chromosome scaffold prep-aration methods with embedment-free section microscopy,we demonstrate that the dino-flagellate Crypthecodinium cohnii chromosome retains a protein scaffold after the depletionof DNA and soluble proteins. This scaffold preserves the morphology characteristic of thechromosome. Two-dimensional electrophoreses show that the chromosome scaffolds are mainlycomposed of acidic proteins. Our results suggest that a framework similar to the chromosomescaffold in the mammalian cell appeared in the primitive eukaryote. We propose that thechromosome scaffold possibly originated from the early stages of eukaryote evolution.
基金the National Natural Science Foundation of China.
文摘The nuclear lamina is a meshwork of intermediate filament-like fibrils situated immediately subjacent to the inner membrane of the nuclear envelope. Together with other proteinaceous elements such as the nuclear pore complex, the lamina forms part of the karyoskeleton, or structural framework of the nucleus. The lamina is thought to play a role in anchoring the nuclear envelope, and/or providing attachment sites for interphase chromatin. Mammalian somatic cells commonly contain three major lamins: A, B and C.
基金Project supported by the National Natural Science Foundation of China and Institute of Biophysics, Chinese Academy of Sciences.
文摘Isolation and characteriation of functional nudear matrix proteins involved in DNA anchoring and gene expression is one of the major subjects of current nudear matrix research. Southwestern blotting (DNA-protein hybridization) was applied to studying the anchoring of c-myc on the nudear matrix proteins in mouse thymic T lymphocytes. The results showed that c-myc bound to the lamin, p34 and p36 nudear matrix proteins specifically. In the process of mouse thymic PNA T lymphocytes proliferation induced by ConA, the anchoring of c-myc on p34 and p36 nudear matrix proteins changed dynamically.
