By employing temporary immersion bioreactor system(TIBs),we studied virus-free culture of seedlings from sugarcane varieties ROC16 and ROC22,from medium recipe,inoculation amount,sucrose concentration,and variety diff...By employing temporary immersion bioreactor system(TIBs),we studied virus-free culture of seedlings from sugarcane varieties ROC16 and ROC22,from medium recipe,inoculation amount,sucrose concentration,and variety difference. The results showed,using this method,that proliferation rate of ROC16 improved by 40 times,per flask generated about 800 plantlets; of ROC22 improved by 30 times,per flask generated about 400-600 plantlets. The results provided basis for using TIBs in rapid propagation of plantlets via tissue culture.展开更多
[Objective]The study was carried out for providing good method to detect ratoon stunting disease（RSD）in virus-free seedcane of Saccharum officinarum.[Method]PCR method was used for the detection of RSD in virus-free...[Objective]The study was carried out for providing good method to detect ratoon stunting disease（RSD）in virus-free seedcane of Saccharum officinarum.[Method]PCR method was used for the detection of RSD in virus-free seedcane of sugarcane.[Result]During the different growth stages of healthy virus-free seedling including proliferation stage,rooting stage,sand culture stage and temporary planting stage,the RSD detection results were negative.PCR detection sensitivity in the present study showed that PCR could detect RSD pathogen in the solution with bacterium concentration 10-3.[Conclusion]PCR method showed a good specificity and higher sensitivity,so it was suitable for the batch detection in the production of healthy virus-free seedlings of sugarcane.展开更多
基金Supported by Youth Science Foundation of Guangxi ( Guikeqing0832060)S&T Development Project from Guangxi Academy of Agricultural Sciences(2006006)~~
文摘By employing temporary immersion bioreactor system(TIBs),we studied virus-free culture of seedlings from sugarcane varieties ROC16 and ROC22,from medium recipe,inoculation amount,sucrose concentration,and variety difference. The results showed,using this method,that proliferation rate of ROC16 improved by 40 times,per flask generated about 800 plantlets; of ROC22 improved by 30 times,per flask generated about 400-600 plantlets. The results provided basis for using TIBs in rapid propagation of plantlets via tissue culture.
基金Supported by Basic Research Found of Guangxi Sugarcane Institute(G2009006)~~
文摘[Objective]The study was carried out for providing good method to detect ratoon stunting disease（RSD）in virus-free seedcane of Saccharum officinarum.[Method]PCR method was used for the detection of RSD in virus-free seedcane of sugarcane.[Result]During the different growth stages of healthy virus-free seedling including proliferation stage,rooting stage,sand culture stage and temporary planting stage,the RSD detection results were negative.PCR detection sensitivity in the present study showed that PCR could detect RSD pathogen in the solution with bacterium concentration 10-3.[Conclusion]PCR method showed a good specificity and higher sensitivity,so it was suitable for the batch detection in the production of healthy virus-free seedlings of sugarcane.
